Construction of an electrochemical DNA chip for simultaneous genotyping of single nucleotide polymorphisms

The Analyst ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 687 ◽  
Author(s):  
Masayoshi Takahashi ◽  
Jun Okada ◽  
Keiko Ito ◽  
Michie Hashimoto ◽  
Koji Hashimoto ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Analysis of multiple candidate genes in association with phenotypes of multiple sclerosis

2010 ◽  
Vol 16 (6) ◽  
pp. 652-659 ◽  
Author(s):  
Madeleine H Sombekke ◽  
David Arteta ◽  
Mark A van de Wiel ◽  
J Bart A Crusius ◽  
Diego Tejedor ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Single Nucleotide Polymorphisms ◽  
Disease Severity ◽  
Clinical Data ◽  
Severity Score ◽  
Interleukin 2 ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Clinical Variables

Multiple sclerosis is a heterogeneous neurological disease with varying degrees of severity. The common hypothesis is that susceptibility to multiple sclerosis and its phenotype are caused by a combination of environmental and genetic factors. The genetic part exerts its effect through several genes, each having modest effects. We evaluated whether disease severity could be predicted by a model based on clinical data and data from a DNA chip. The DNA chip was designed containing several single nucleotide polymorphisms in 44 genes, previously described to be associated with multiple sclerosis. A total of 605 patients with multiple sclerosis were included in this analysis, using gender, onset type and age at onset as clinical covariates. We correlated 80 single nucleotide polymorphisms to the degree of disease severity using the following three outcome measures: linear Multiple Sclerosis Severity Score, dichotomous Multiple Sclerosis Severity Score (using a cut-off point of 2.5) and time to reach Expanded Disability Status Scale score 6. Sixty-nine single nucleotide polymorphisms were included in the analysis. No individual single nucleotide polymorphism showed a significant association; however, a combination of single nucleotide polymorphisms significantly improved the prediction of disease severity in addition to the clinical variables. In all three models the Interleukin 2 gene was included, confirming a previously reported modest effect on disease severity. The highest power was obtained using the dichotomized Multiple Sclerosis Severity Score as outcome. Several single nucleotide polymorphisms showed their added predictive value over the clinical data in the predictive models. These results support our hypothesis that disease severity is determined by clinical variables and genetic influences (through several genes with small effects) in concert.

Simultaneous Determination of Single Nucleotide Polymorphisms of MDR1 Genes by Electrochemical DNA Chip

2005 ◽  
Vol 20 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Tsutomu Nakamura ◽  
Toshiyuki Sakaeda ◽  
Masayoshi Takahashi ◽  
Koji Hashimoto ◽  
Nobuhiro Gemma ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Simultaneous Determination ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip

Microarrays ◽  
2007 ◽  
pp. 267-278
Author(s):  
Alexandre Ho-Pun-Cheung ◽  
Hafid Abaibou ◽  
Philippe Cleuziat ◽  
Evelyne Lopez-Crapez
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip

Microarrays ◽  
2007 ◽  
pp. 267-278
Author(s):  
Alexandre Ho-Pun-Cheung ◽  
Hafid Abaibou ◽  
Philippe Cleuziat ◽  
Evelyne Lopez-Crapez
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Detection of single nucleotide polymorphisms by minisequencing on a polypyrrole DNA chip designed for medical diagnosis

2006 ◽  
Vol 86 (3) ◽  
pp. 304-313 ◽  
Author(s):  
Alexandre Ho-Pun-Cheung ◽  
Sylvie Choblet ◽  
Thomas Colineau ◽  
Hafid Abaibou ◽  
Daniela Zsoldos ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Medical Diagnosis ◽  
Dna Chip ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Development of a simple method for the determination of single nucleotide polymorphisms

2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Simple Method ◽  
Single Nucleotide
Sign in / Sign up

Export Citation Format

Share Document