Determination of environmentally important metal ions by fluorescence quenching in anionic micellar solution

The Analyst ◽  
2005 ◽  
Vol 130 (2) ◽  
pp. 242-246 ◽  
Author(s):  
Leonardo V. Vargas ◽  
Juergen Sand ◽  
Tiago A. S. Brandão ◽  
Haidi D. Fiedler ◽  
Frank H. Quina ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Metal Ions ◽  
Micellar Solution

A Homogeneous Nonisotopic Histone Deacetylase Activity Assay

2003 ◽  
Vol 8 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Birgit Heltweg ◽  
Manfred Jung
Keyword(s):  
Drug Discovery ◽  
Fluorescence Quenching ◽  
Histone Deacetylase ◽  
Anticancer Agents ◽  
Histone Deacetylases ◽  
Activity Assay ◽  
Animal Testing ◽  
Hdac Activity

Histone deacetylases (HDACs) are important regulators of transcription, and their inhibitors are a promising class of anticancer agents. The methods for the determination of HDAC activity and its inhibition that are currently available suffer from various drawbacks, such as animal testing, radioactive substrates, or limited throughput. Therefore, a fast nonisotopic method for the measurement of HDAC activity is highly desirable. The authors present such an assay that relies on the fluorescent HDAC substrate developed previously in their group. After incubation of the substrate with the enzyme, a derivatization leads to efficient fluorescence quenching in the deacetylated metabolite. Thus, only the fluorescence emitted by the remaining substrate is detected, which allows for a convenient detection of HDAC activity in a homogeneous format that can be performed on multiwell plate readers. This procedure, called HDASH (histone deacetylase assay—homogeneous), should be a valuable tool in transcriptional research and especially drug discovery. ( Journal of Biomolecular Screening 2003:89-95)

Simple semiquantitative determination of trace metal ions by use of reagent gel columns—II

Talanta ◽  
1976 ◽  
Vol 23 (3) ◽  
pp. 244-246 ◽  
Author(s):  
Yong Keun Lee ◽  
Kyu Ja Whang ◽  
Keihei Ueno
Keyword(s):  
Trace Metal ◽  
Metal Ions ◽  
Trace Metal Ions ◽  
Semiquantitative Determination

Determination of Sodium Hexametaphosphate in Beverages Based on the Fluorescence Quenching of Acridine Orange

2014 ◽  
Vol 47 (16) ◽  
pp. 2740-2746
Author(s):  
Heng Xin Zhao ◽  
Ming Luo ◽  
Li Xin Mo ◽  
Liang Cheng ◽  
Zheng Ma ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Sodium Hexametaphosphate
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