Hyphenating liquid phase separation techniques with mass spectrometry: on-line or off-line

The Analyst ◽  
2005 ◽  
Vol 130 (1) ◽  
pp. 13 ◽  
Author(s):  
Emma Edwards ◽  
Jane Thomas-Oates
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Separation Techniques ◽  
On Line

Use of the kinetic plot method to compare the efficiency and resolution of liquid-phase separation techniques based on different driving forces

2010 ◽  
Vol 23 (6) ◽  
pp. 440-446 ◽  
Author(s):  
Veronika Fekete ◽  
Ágnes Fekete ◽  
Jenő Fekete ◽  
Anuschka Liekens ◽  
Philippe Schmitt-Kopplin ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Driving Forces ◽  
Liquid Phase Separation ◽  
Separation Techniques

A two-dimensional liquid-phase separation method coupled with mass spectrometry for proteomic studies of breast cancer and biomarker identification

PROTEOMICS ◽  
2004 ◽  
Vol 4 (3) ◽  
pp. 562-577 ◽  
Author(s):  
Rick L. Hamler ◽  
Kan Zhu ◽  
Nathan S. Buchanan ◽  
Paweena Kreunin ◽  
Maureen T. Kachman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Separation Method ◽  
Two Dimensional ◽  
Biomarker Identification

Recent technical progress in sample preparation and liquid-phase separation-mass spectrometry for proteomic analysis of mass-limited samples

2021 ◽  
Vol 13 (10) ◽  
pp. 1214-1225
Author(s):  
Zhichang Yang ◽  
Liangliang Sun
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Liquid Phase ◽  
Sample Preparation ◽  
Technical Progress ◽  
Proteomic Analysis ◽  
Liquid Phase Separation ◽  
Preparation Methods ◽  
Sample Preparation Methods

Recent development of sample preparation methods with nanoliter processing volumes, ultrasensitive LC-MS/MS and CZE-MS/MS systems for proteomics of mass-limited samples.

Protein Fingerprints of Anti-cancer Effects of Cyclin-dependent Kinase Inhibition: Identification of Candidate Biomarkers Using 2-D Liquid Phase Separation Coupled to Mass Spectrometry

2005 ◽  
Vol 4 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Helena Skalnikova ◽  
Petr Halada ◽  
Petr Dzubak ◽  
Marian Hajduch ◽  
Hana Kovarova
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Liquid Phase ◽  
Protein Expression ◽  
Anticancer Activity ◽  
Histone Variants ◽  
Liquid Phase Separation ◽  
Cyclin Dependent Kinase ◽  
Kinase Inhibition ◽  
Anti Cancer

The purpose of this study was to apply a recently introduced proteomic based approach to identify candidate biomarkers of the response to anticancer activity of cyclin-dependent kinase inhibitor, bohemine. Mapping of the total protein expression of CEM lymphoblastic leukemia cells following bohemine treatment was performed by 2-D liquid phase separation. Proteins were fractionated by isoelectric points in pH gradient in the first dimension and each of these pI protein fractions was further separated by hydrophobicity using non-porous silica reverse phase chromatography in the second dimension. 2-D protein expression maps of control untreated and bohemine treated cells were generated and inter-sample comparison was performed. Most of the differentially expressed proteins were present at a decreased level after bohemine treatment while there were four proteins, which were up regulated. These proteins representing candidate biomarkers of cancer cell response to the treatment were selected for identification by mass spectrometry. Our results demonstrating down regulation of three histone variants, different in their pI and hydrophobicity, in response to bohemine indicated that anti-mitotic and anti-cancer activities of this compound may be associated with epigenetic regulation at the level of chromatin structure. Furthermore, crk-like adaptor scaffolding protein represents a new important protein family affected by bohemine. This strategy is valuable for comprehensive proteomic analysis of cellular protein targets and pathways that are relevant to anticancer activity of cyclin-dependent kinase inhibition.

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