Laser-assisted fluorescence microscopy for measuring cell membrane dynamics

2004 ◽  
Vol 3 (8) ◽  
pp. 817 ◽  
Author(s):  
Herbert Schneckenburger ◽  
Michael Wagner ◽  
Martina Kretzschmar ◽  
Wolfgang S. L. Strauss ◽  
Reinhard Sailer
Keyword(s):  
Cell Membrane ◽  
Fluorescence Microscopy ◽  
Membrane Dynamics ◽  
Measuring Cell

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

scholarly journals Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies

2007 ◽  
Vol 179 (5) ◽  
pp. 1067-1082 ◽  
Author(s):  
Valeria R. Caiolfa ◽  
Moreno Zamai ◽  
Gabriele Malengo ◽  
Annapaola Andolfo ◽  
Chris D. Madsen ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Plasminogen Activator ◽  
Fluorescent Protein ◽  
Surface Protein ◽  
Basal Membrane ◽  
Membrane Dynamics ◽  
Live Cells ◽  
Cell Surface Protein ◽  
Protein Assemblies

To search for functional links between glycosylphosphatidylinositol (GPI) protein monomer–oligomer exchange and membrane dynamics and confinement, we studied urokinase plasminogen activator (uPA) receptor (uPAR), a GPI receptor involved in the regulation of cell adhesion, migration, and proliferation. Using a functionally active fluorescent protein–uPAR in live cells, we analyzed the effect that extracellular matrix proteins and uPAR ligands have on uPAR dynamics and dimerization at the cell membrane. Vitronectin directs the recruitment of dimers and slows down the diffusion of the receptors at the basal membrane. The commitment to uPA–plasminogen activator inhibitor type 1–mediated endocytosis and recycling modifies uPAR diffusion and induces an exchange between uPAR monomers and dimers. This exchange is fully reversible. The data demonstrate that cell surface protein assemblies are important in regulating the dynamics and localization of uPAR at the cell membrane and the exchange of monomers and dimers. These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents.

TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy

2012 ◽  
pp. 439-455
Author(s):  
Felix Neugart ◽  
Darius Widera ◽  
Barbara Kaltschmidt ◽  
Christian Kaltschmidt ◽  
Mike Heilemann
Keyword(s):  
Fluorescence Microscopy ◽  
Membrane Dynamics ◽  
Tnf Receptor ◽  
Receptor Membrane

scholarly journals Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells

2020 ◽  
Vol 11 (3) ◽  
pp. 702-715 ◽  
Author(s):  
Michael Gorry ◽  
Toshie Yoneyama ◽  
Lazar Vujanovic ◽  
Marcia L. Moss ◽  
Michelle A. Garlin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Enzyme Activity ◽  
Cell Membrane ◽  
Measuring Cell ◽  
Membrane Enzyme

scholarly journals Application of Spot Variation FCS (svFCS) Analysis to T Cell Membrane Dynamics

2020 ◽  
Vol 118 (3) ◽  
pp. 353a
Author(s):  
Yannick Hamon ◽  
Anne-Marie Sartre ◽  
Anthony Formisano ◽  
Sébastien Mailfert ◽  
Didier Marguet ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Membrane ◽  
Membrane Dynamics ◽  
T Cell Membrane

Cholesterol dependence of cell membrane dynamics

2005 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Reinhard Sailer ◽  
Wolfgang S. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Membrane Dynamics

Electrical Resistance and Volume Flow in Glass Microelectrodes

1971 ◽  
Vol 49 (5) ◽  
pp. 436-447 ◽  
Author(s):  
D. R. Firth ◽  
L. J. DeFelice
Keyword(s):  
Cell Membrane ◽  
Electrical Resistance ◽  
Surface Condition ◽  
External Solution ◽  
Membrane Resistance ◽  
Volume Flow ◽  
Glass Wall ◽  
Measuring Cell ◽  
Cell Membrane Resistance ◽  
Flow Effects

The electrical resistance and the flow of solution in glass microelectrodes has been studied as function of the concentration of internal and external solution (KCl, 3–10−5 M), and as a function of pressure between the inside and outside solutions. The study demonstrates the usefulness of using microelectrodes for measuring cell membrane resistance and also the dangers in using them as microejectors because of anomalous flow effects. Electrokinetic effects and tip potentials are briefly described; they offer a means of investigating the surface condition of the glass wall inside the tip of the electrode and its variation with the outside solution.

scholarly journals 2P213 Scanning ion conductance microscopy for measuring single cell membrane fluctuations(13B. Biological & Artifical membrane: Dynamics,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S194
Author(s):  
Zen Ishikura ◽  
Yusuke Mizutani ◽  
Kaori Kuribayashi-Shigetomi ◽  
Yuuki Fujii ◽  
Myung-Hoon Choi ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Dynamics ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations
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