Surface-enhanced resonance Raman scattering as an analytical tool for single molecule detection

The Analyst ◽  
2004 ◽  
Vol 129 (4) ◽  
pp. 337 ◽  
Author(s):  
Benissa Tolaieb ◽  
Carlos J. L. Constantino ◽  
Ricardo F. Aroca
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Analytical Tool ◽  
Single Molecule Detection ◽  
Resonance Raman Scattering ◽  
Surface Enhanced

Single-Molecule Detection Using Surface-Enhanced Resonance Raman Scattering and Langmuir−Blodgett Monolayers

2001 ◽  
Vol 73 (15) ◽  
pp. 3674-3678 ◽  
Author(s):  
Carlos J. L. Constantino ◽  
Tibebe Lemma ◽  
Patricia A. Antunes ◽  
Ricardo Aroca
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Single Molecule Detection ◽  
Resonance Raman Scattering ◽  
Langmuir Blodgett ◽  
Surface Enhanced

Approach to Single Molecule Detection Using Surface-Enhanced Resonance Raman Scattering (SERRS): A Study Using Rhodamine 6G on Colloidal Silver

1995 ◽  
Vol 49 (6) ◽  
pp. 780-784 ◽  
Author(s):  
Katrin Kneipp ◽  
Yang Wang ◽  
Ramachandra R. Dasari ◽  
Michael S. Feld
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Rhodamine 6G ◽  
Detection System ◽  
Resonance Raman ◽  
Single Molecule Detection ◽  
Colloidal Silver ◽  
Fiber Optic Probe ◽  
Resonance Raman Scattering ◽  
Surface Enhanced

We have measured surface-enhanced resonance Raman scattering (SERRS) spectra of rhodamine 6G (R6G) at concentrations as low as 8 × 10−16 M in colloidal silver solution activated by NaCl ions. The spectra were measured with a fiber-optic probe using the 514.5-nm argon-ion laser line as excitation source and a charge-coupled-device (CCD) detection system. The correlation of SERRS photo counts and R6G concentration was found to be linear between 8 × 10−11 and 8 × 10−14 M concentrations within our experimental accuracy. Experiments conducted with small scattering volumes show that fewer than 100 R6G molecules are sufficient to give rise to a SERRS spectrum with reasonable signal-to-noise ratio. These results demonstrate that in certain cases SERRS can achieve detection limits comparable to those for fluorescence spectroscopy, and at the same time provides higher structural specificity than fluorescence. The possibilities of using SERRS for single molecule detection are discussed.

Surface-Enhanced Resonance Raman Scattering: Single-Molecule Detection in a Langmuir—Blodgett Monolayer

2003 ◽  
Vol 57 (6) ◽  
pp. 649-654 ◽  
Author(s):  
Carlos J. L. Constantino ◽  
Tibebe Lemma ◽  
Patricia A. Antunes ◽  
Paul Goulet ◽  
Ricardo Aroca
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Arachidic Acid ◽  
Resonance Raman ◽  
Single Molecule Detection ◽  
Resonance Raman Scattering ◽  
Surface Enhanced Fluorescence ◽  
Langmuir Blodgett ◽  
Spatially Resolved ◽  
Surface Enhanced

Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular layer containing one BZP molecule per μm2, which is also the probing area of the Raman microscope. For concentrated samples (attomole quantities in the field of view), average SERRS, surface-enhanced fluorescence (SEF), and Raman imaging, including line mapping and global images at different temperatures, were recorded. Single-molecule SERRS spectra, obtained using an LB monolayer, present changes in bandwidth and relative intensities, highlighting the properties of single-molecule SERRS that are lost in average SERRS measurements of mixed LB monolayers obtained at the same temperatures. Also, the dilute system phenomenon of blinking is discussed with regard to results obtained from LB monolayers. The dilution process used in the single-molecule LB SERRS work is independently supported by fluorescence results obtained from very dilute solutions with monomer concentrations down to 10−12 M.

Single molecule level detection of allophycocyanin by surface enhanced resonance Raman scattering

The Analyst ◽  
2007 ◽  
Vol 132 (7) ◽  
pp. 633 ◽  
Author(s):  
Colin D. McGuinness ◽  
Alexander M. Macmillan ◽  
Jan Karolin ◽  
W. Ewen Smith ◽  
Duncan Graham ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Resonance Raman Scattering ◽  
Single Molecule Level ◽  
Surface Enhanced

scholarly journals 2P312 Localized single molecule surface enhanced resonance raman scattering

2005 ◽  
Vol 45 (supplement) ◽  
pp. S197
Author(s):  
K. Watanabe ◽  
M. Tsunoda ◽  
T. Ueno ◽  
M. Yamagishi ◽  
T. Funatsu
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Molecule Surface
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