Structural study by transmission and scanning electron microscopy of the time-dependent structural change in M41S mesoporous silica (MCM-41 to MCM-48, and MCM-50)

2004 ◽  
Vol 14 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Isabel Díaz ◽  
Joaquin Pérez-Pariente ◽  
Osamu Terasaki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Structural Change ◽  
Mesoporous Silica ◽  
Structural Study ◽  
Time Dependent ◽  
Mcm 41 ◽  
Scanning Electron

scholarly journals The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Zhenwei Han ◽  
Xuan Lu ◽  
Yiping Tang ◽  
Yuanyuan Yang ◽  
Qiuchen Liu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Monoclonal Antibody ◽  
Time Dependent ◽  
The Novel ◽  
Plasma Samples ◽  
Novel Therapy ◽  
Real Time Quantitative Pcr ◽  
Therapy Option ◽  
Scanning Electron

Objective. To establish a novel HBV specific immunoadsorbent for the removing of HBV particles.Methods. The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microscopy. In addition, time-dependent effects of the eradication capacity of anti-HBsAg functionalized sepharose beads against HBV were investigated.Results. Proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. With the optimized recycle time, the decontamination performance of HBV particles and quantity of HBsAg were assessed either by real-time quantitative PCR or ELISA, which showed that the immunoadsorbent could remove approximately 90% of the HBV and 90% of the HBsAg from human plasma samples.Conclusions. All these results indicated that the novel immunoadsorbent could effectively remove HBV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HBV.

scholarly journals Morphological Characterization of Ordered Meso-Pores on a Mesoporous Silica by using High-Resolution Scanning Electron Microscopy

2011 ◽  
Vol 17 (S2) ◽  
pp. 1912-1913
Author(s):  
Y Ohrai ◽  
T Sunaoshi ◽  
H Ito ◽  
T Ogashiwa ◽  
N Ikawa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Mesoporous Silica ◽  
Morphological Characterization ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Effect of glyphosate spray droplets on leaf cytology in velvetleaf (Abutilon theophrasti)

Weed Science ◽  
2004 ◽  
Vol 52 (2) ◽  
pp. 302-309 ◽  
Author(s):  
Jan S. Ryerse ◽  
Roger A. Downer ◽  
R. Douglas Sammons ◽  
Paul C. C. Feng
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Structural Change ◽  
Abutilon Theophrasti ◽  
Leaf Epidermis ◽  
Contact Site ◽  
Cellular Changes ◽  
Spray Droplets ◽  
Droplet Sizes ◽  
Scanning Electron

Leaf cytoarchitecture was evaluated by light microscopy and scanning electron microscopy, and cell viability was monitored by fluorescence after treatment of velvetleaf with defined concentrations and droplet sizes of formulated glyphosate and blended tallowamine surfactant. In response to droplets of formulated glyphosate larger than in field sprays but useful for studying structural change, we observe that the leaf epidermis thins and flattens within 1.5 h, the epidermal, mesophyll, and vascular cells at the contact site exhibit localized cytolysis by 6 h, and cytolysis and pycnosis remain restricted to the contact site at 24 h. Using endogeneous fluorescence as a marker for nonviable cells, it was determined that cellular changes are directly correlated with droplet size and that the changes are minimal after exposure to spray sizes and concentrations of formulated glyphosate and blended tallowamine typically used in the field. The results show that, at field use concentrations, the effect of formulated glyphosate and blended tallowamine on leaf cytoarchitecture is modest and localized but sufficient to allow herbicide entry.

scholarly journals The inhibition of human platelet function by ganodermic acids

1991 ◽  
Vol 277 (1) ◽  
pp. 189-197 ◽  
Author(s):  
C N Wang ◽  
J C Chen ◽  
M S Shiao ◽  
C T Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Time Dependent ◽  
Second Phase ◽  
Phosphoinositide Metabolism ◽  
Prolonged Incubation ◽  
Dependent Inhibition ◽  
Time Dependent Inhibition ◽  
Inhibition Of Thrombin ◽  
Scanning Electron

Human gel-filtered platelets aggregate at greater than 20 microM-ganodermic acid S [lanosta-7,9(11),24-triene-3 beta, 15 alpha-diacetoxy-26-oic acid] [Wang, Chen, Shiao & Wang (1989) Biochim. Biophys. Acta 986, 151-160]. This study showed that platelets at less than 20 microM-ganodermic acid S displayed both concentration- and time-dependent inhibition of function, in which the agent potency in response to inducers was ADP-fibrinogen greater than collagen greater than thrombin. The agent caused a biphasic time-dependent effect on platelet phosphoinositide metabolism. The first phase involved the decrease in the pool size of phosphoinositide by 10-20%. The second phase, in which both the resynthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) and the decrease of [32P]phosphatidic acid occurred, took place after 30 min. Scanning electron microscopy also revealed a time-dependent morphological change in platelets in the presence of the agent. The cells initially became spiculate discs, then swelled to a ‘potato-like’ morphology at 60 min. Further studies on the time-dependent inhibition of thrombin response revealed that: (1) the percentage inhibition of cell aggregation was comparable with that occurring with an increase of cytosolic free Ca2+ concentration [(Ca2+]i) or the phosphorylation of marker proteins; (2) [32P]Pi-labelled platelets showed the time-dependent inhibition of thrombin-stimulated PIP2 resynthesis as indicated by first-2-min time-course studies of phosphoinositide interconversion; (3) scanning electron microscopy revealed that the aged platelet population showed an increase in the percentage of non-responding cells on prolonged incubation. The results, taken together, enabled one to discuss a possible mechanism for the time-dependent inhibition by ganodermic acid S of platelet response to thrombin.

scholarly journals Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM)

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 222
Author(s):  
María José Gómez-Torres ◽  
Natalia Huerta-Retamal ◽  
Laura Robles-Gómez ◽  
Paula Sáez-Espinosa ◽  
Jon Aizpurua ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Emission ◽  
Human Sperm ◽  
Time Dependent ◽  
Arylsulfatase A ◽  
Surface Mapping ◽  
Heterogeneous Distribution ◽  
Scanning Electron

Capacitation drives sperm biophysical and biochemical changes for sperm-oocyte interactions. It is a well-known fact that the molecular complex arylsulfatase A (ARSA), hyaluronidase sperm adhesion molecule 1 (SPAM1), and heat shock protein 2 (HSPA2) plays a significant role in sperm–zona pellucida (ZP) binding. However, the time-dependent capacitation effects on the sperm surface ARSA presence and specific topographic distributions remain to be elucidated. Here, we quantified the ARSA density and specific membrane domain locations before (US) and after in vitro capacitation (one and four hours; CS1–CS4) in human sperm using high-resolution field emission scanning electron microscopy (FE-SEM) and immunogold labeling. Our results showed a significant and progressive capacitation-mediated increase of labeled spermatozoa from the US (37%) to CS4 (100%) physiological conditions. In addition, surface mapping revealed a close relationship between the ARSA residues and their acrosomal repositioning. Compared with the ARSA surface heterogeneous distribution found in US, the CS1–4 conditions exhibited clustering on the peri-acrosomal region, showing that time-dependent capacitation also induced a ARSA residue dramatic translocation on sperm surfaces. Our findings provide novel insights into the molecular remodeling events preceding sperm-oocyte interactions.

Radiation damage of mesoporous silica thin films monitored by X-ray reflectivity and scanning electron microscopy

2012 ◽  
Vol 427 (1-3) ◽  
pp. 411-414 ◽  
Author(s):  
S. Dourdain ◽  
X. Deschanels ◽  
G. Toquer ◽  
C. Grygiel ◽  
I. Monnet ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Scanning Electron Microscopy ◽  
Mesoporous Silica ◽  
Radiation Damage ◽  
X Ray ◽  
Silica Thin Films ◽  
Scanning Electron
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