The ion gating effect: using a change in flexibility to allow label free electrochemical detection of DNA hybridisationElectronic supplementary information (ESI) available: CVs: ss-DNA modified electrode exposed to a non-complementary sequence; MCE-modified gold electrode. See http://www.rsc.org/suppdata/cc/b3/b305798b/

2003 ◽  
pp. 1938 ◽  
Author(s):  
J. Justin Gooding ◽  
Alison Chou ◽  
Freya J. Mearns ◽  
Elicia (Leh-See) Wong ◽  
Kellie L. Jericho
Keyword(s):  
Modified Electrode ◽  
Electrochemical Detection ◽  
Gold Electrode ◽  
Supplementary Information ◽  
Label Free ◽  
Complementary Sequence ◽  
Gating Effect

Tethering of spherical DOTAP liposome gold nanoparticles on cysteamine monolayer for sensitive label free electrochemical detection of DNA and transfection

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2467-2475 ◽  
Author(s):  
Mohanlal Bhuvana ◽  
Venkataraman Dharuman
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Detection ◽  
Gold Electrode ◽  
Label Free

Cysteamine monolayer supported spherical DOTAP–AuNP on gold electrode is developed for DNA label free sensing and transfection.

Label-free electrochemical detection of DNA hybridization on gold electrode

2003 ◽  
Vol 5 (10) ◽  
pp. 887-891 ◽  
Author(s):  
Kagan Kerman ◽  
Yasutaka Morita ◽  
Yuzuru Takamura ◽  
Eiichi Tamiya
Keyword(s):  
Electrochemical Detection ◽  
Dna Hybridization ◽  
Gold Electrode ◽  
Label Free

scholarly journals Electrochemical Detection of Dopamine at a Gold Electrode Modified with a Polypyrrole–Mesoporous Silica Molecular Sieves (MCM-48) Film

2018 ◽  
Vol 20 (1) ◽  
pp. 111 ◽  
Author(s):  
Izabela Zablocka ◽  
Monika Wysocka-Zolopa ◽  
Krzysztof Winkler
Keyword(s):  
Ascorbic Acid ◽  
Cyclic Voltammetry ◽  
Detection Limit ◽  
Modified Electrode ◽  
Electrochemical Detection ◽  
Molecular Sieves ◽  
Gold Electrode ◽  
Square Wave Voltammetry ◽  
Wave Voltammetry

A gold electrode modified with a polypyrrole–mesoporous silica molecular sieves (polypyrrole—MCM-48) nanostructure film was used for the electrochemical determination of small concentrations of dopamine (DA) by cyclic voltammetry and square-wave voltammetry techniques. This electrode showed good electrocatalytic activity for the oxidation of dopamine. The oxidation potential of dopamine was decreased significantly compared with that obtained at the bare gold electrode. The observed linear range for the determination of the dopamine concentration, without interferents through cyclic voltammetry measurements, was from 10 μM to 1.2 mM (R2 = 0.9989) for the gold electrode modified with the polypyrrole—MCM-48 nanostructure, with a detection limit of 2.5 μM. In the case of square-wave voltammetry, the linear range was 2–250 μM, with a correlation coefficient of 0.9996, and the detection limit was estimated to be 0.7 μM. The effects of interferents, such as ascorbic acid (AA) and uric acid (UA), on the electrochemical detection of dopamine were also examined. The modified electrode can successfully separate the oxidation potentials for ascorbic acid and dopamine, shifting the oxidation peak potential of ascorbic acid to a more positive potential, and significantly decreasing the peak current. The presence of ascorbic acid increased the sensitivity of dopamine determination at the modified electrode, and the detection limit was estimated to be 0.5 μM with 0.1 mM ascorbic acid to imitate physiological solutions. Additionally, studies showed that the presence of uric acid does not affect the electrochemical detection of dopamine. The modified electrode can be successfully applied for the quantitative analysis of dopamine both with and without interferents.

scholarly journals Label-Free Electrochemical Test of Protease Interaction with a Peptide Substrate Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 199
Author(s):  
Anna Wcisło ◽  
Izabela Małuch ◽  
Paweł Niedziałkowski ◽  
Tadeusz Ossowski ◽  
Adam Prahl
Keyword(s):  
Gold Electrode ◽  
High Selectivity ◽  
Electrode Materials ◽  
Electrochemical Biosensors ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Peptide Substrate ◽  
Electrochemical Test ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method

Efficient deposition of biomolecules on the surface, maintaining their full activity and stability, is a most significant factor in biosensor construction. For this reason, more and more research is focused on the development of electrochemical biosensors that have the ability to electrically detect adsorbed molecules on electrode surface with high selectivity and sensitivity. The presented research aims to develop an efficient methodology that allows quantification of processes related to the evaluation of enzyme activity (proprotein convertase) using electrochemical methods. In this study we used impedance spectroscopy to investigate the immobilization of peptide substrate (Arg-Val-Arg-Arg) modified with 11-mercaptoundecanoic acid on the surface of gold electrode. Both the synthesis of the peptide substrate as well as the full electrochemical characteristics of the obtained electrode materials have been described. Experimental conditions, including concentration of peptide substrate immobilization, modification time, linker, and the presence of additional blocking groups have been optimized. The main advantages of the described method is that it makes it possible to observe the peptide substrate–enzyme interaction without the need to use fluorescent labels. This also allows observation of this interaction at a very low concentration. Both of these factors make this new technique competitive with the standard spectrofluorimetric method.

Construction of electrochemical immunosensor based on redox hydrogel for ultrasensitive detection of carcinoembryonic antigen

2021 ◽  
Author(s):  
Xiangrong Huang ◽  
Na Wu ◽  
Wenxiu Liu ◽  
Yazhuo Shang ◽  
Honglai Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Detection ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free

In this work, a novel redox hydrogel was proposed for ultrasensitive label-free electrochemical detection of carcinoembryonic antigen (CEA). The redox hydrogel composed by cellulose nanocrystalline (CNC), methylene blue (MB), multi-walled...

A label-free ratiometric immunoassay using bioinspired nanochannels and a smart modified electrode

2021 ◽  
pp. 338476
Author(s):  
Zhe Qiao ◽  
Zilian Jiang ◽  
Qiufen Luo ◽  
Hongfang Zhang ◽  
Jianbin Zheng
Keyword(s):  
Modified Electrode ◽  
Label Free
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