A flow injection online micelle-mediated preconcentration and separation procedure without phase separation coupled with electrothermal atomic absorption spectrometry for determination of trace lead in biological samples

2003 ◽  
Vol 18 (8) ◽  
pp. 946 ◽  
Author(s):  
Jing Nan ◽  
Yan Jiang ◽  
Xiu-Ping Yan
Keyword(s):  
Phase Separation ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flow Injection ◽  
Biological Samples ◽  
Electrothermal Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Separation Procedure

Evaluation of arsenic, cobalt, copper and manganese in biological Samples of Steel mill workers by electrothermal atomic absorption Spectrometry

2009 ◽  
Vol 25 (1) ◽  
pp. 59-69 ◽  
Author(s):  
HI Afridi ◽  
TG Kazi ◽  
NG Kazi ◽  
MK Jamali ◽  
MB Arain ◽  
...  
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Biological Samples ◽  
Electrothermal Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Human Beings ◽  
Acid Digestion ◽  
Steel Mill ◽  
Hair Samples

The determination of trace and toxic elements in biological samples (blood, urine and scalp hair samples) of human beings is an important clinical test. The aim of our present study was to determine the concentration of arsenic (As), copper (Cu), cobalt (Co) and manganese (Mn), in biological samples of male production workers (PW) and quality control workers (QW) of steel mill, with aged 25–55 years, to assess the possible influence of environmental exposure. For comparison purpose, the same biological samples of unexposed healthy males of same age group were collected as control subjects. The determination of all elements in biological samples was carried out by electrothermal atomic absorption spectrometry, prior to microwave assisted acid digestion. The accuracy of the As, Cu, Co and Mn measurements was tested by simultaneously analyzing certified reference materials (CRMs) and for comparative purposes conventional wet acid digestion method was used on the same CRMs. No significant differences were observed between the analytical results and the certified values, using both methods (paired t-test at P > 0.05). The results indicate that concentrations of As, Cu, Co and Mn in all three biological samples of the exposed workers (QW and PW) were significantly higher than those of the controls. The possible correlation of these elements with the etiology of different physiological disorders is discussed. The results were also demonstrated the need of attention for improvements in workplace, ventilation and industrial hygiene practices.

scholarly journals Determination of Inorganic Arsenic in Marine Food Samples by Hydrochloric Acid Distillation and Flow-Injection Hydride-Generation Atomic Absorption Spectrometry

1999 ◽  
Vol 82 (5) ◽  
pp. 1217-1223 ◽  
Author(s):  
Joar Karsten Øygard ◽  
Anne-Katrine Lundebye ◽  
Kåre Julshamn
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flow Injection ◽  
Biological Samples ◽  
Certified Reference Materials ◽  
Hydride Generation ◽  
Absorption Spectrometry ◽  
Fish Fillet ◽  
Inorganic As

Abstract A simple, rapid, and reliable method was developed for determination of inorganic As in biological samples such as fish fillet. Inorganic AS was distilled from the sample as AsCl3 with HCI. The separated inorganic AS was determined by flow-injection hydride-generation atomic absorption spectrometry after prereduction with Kl and HCI. The influences of various concentrations of Kl, ascorbic acid, and HCI in the prereduction stage; NaBH4 as the reductant; and HCI as the carrier solution on analytical results were studied. Digestion was performed in a Kjeldahl digestion system for 75 min with 4 mL nitric acid and 1 mL sulfuric acid at 380°C. The concentrations of inorganic As in samples were less than 0.1 mg/kg dry weight for fish fillet and somewhat higher for crustaceans and bivalve molluscs. The total and inorganic As contents of various marine biological samples and certified reference materials were determined.

scholarly journals Determination of Cadmium and Lead in Biological Samples by Three Ultrasonic-Based Samples Treatment Procedures Followed by Electrothermal Atomic Absorption Spectrometry

2007 ◽  
Vol 90 (2) ◽  
pp. 470-478 ◽  
Author(s):  
Hassan I Afridi ◽  
Tasneem G Kazi ◽  
Mohammad B Arain ◽  
Mohammad K Jamali ◽  
Gul H Kazi ◽  
...  
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Biological Samples ◽  
Human Hair ◽  
Electrothermal Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Bovine Liver ◽  
Sample Treatment ◽  
Ultrasonic Assisted

Abstract The development of 3 different ultrasonic-based sample treatment methods, ultrasonic probe-assisted acid extraction, ultrasonic-assisted acid slurry, and ultrasonic-assisted acid pseudodigestion is presented. These methods were compared for the determination of Cd and Pb by electrothermal atomic absorption spectrometry in biological samples (blood and scalp hair) and validated by using certified materials BCR 397 human hair and BCR 185R bovine liver. The sample amounts chosen to perform the analysis were 100 mg and 0.5 mL for solids (human hair and bovine liver) and blood samples, respectively. An acid digestion induced by microwave energy was used to obtain the total metal concentrations and for comparative purposes. The best results were obtained with the ultrasonic-assisted acid pseudodigestion, with which it was possible to perform accurate and precise determination of the Cd and Pb contents in 2 certified reference materials and biological samples of 50 normal males of ages 2540 years. The precision of the methods, together with their efficiency, rapidity, low cost, and environmental acceptability, make them good alternatives for the determination of trace metals from biological samples. The precision of the methods for accuracy evaluation, resulting in good agreement according to the t-test for a 95% confidence level, and the relative standard deviations were lower than 10% (n = 10) for all determinations.

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