Evaluation of chemometric methods for determining the number and position of components in high-performance liquid chromatography detected by diode array detector and on-flow 1H nuclear magnetic resonance spectroscopy

The Analyst ◽  
2003 ◽  
Vol 128 (8) ◽  
pp. 1082 ◽  
Author(s):  
Mohammad Wasim ◽  
Mohd Sukri Hassan ◽  
Richard G Brereton
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Array Detector ◽  
Resonance Spectroscopy ◽  
Diode Array ◽  
Chemometric Methods ◽  
Diode Array Detector ◽  
1H Nuclear Magnetic Resonance

scholarly journals Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Thiyagarajan Sathishkumar ◽  
Ramakrishnan Baskar ◽  
Mohan Aravind ◽  
Suryanarayanan Tilak ◽  
Sri Deepthi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Shake Flask ◽  
Orthogonal Design ◽  
Array Detector ◽  
Diode Array ◽  
Ionization Mass ◽  
Diode Array Detector

Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2±0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

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