Formation and destruction of the guanine quartet in solution observed by cold-spray ionization mass spectrometryElectronic supplementary information available: CSI and ESI mass spectra of dG, dC, dA and dT, and schematic diagram of the coldspray ion source. See http://www.rsc.org/suppdata/cc/b2/b212432g/

2003 ◽  
pp. 788-789 ◽  
Author(s):  
Shigeru Sakamoto ◽  
Kazuhiko Nakatani ◽  
Isao Saito ◽  
Kentaro Yamaguchi
Keyword(s):  
Cold Spray ◽  
Mass Spectra ◽  
Ion Source ◽  
Supplementary Information ◽  
Ionization Mass ◽  
Schematic Diagram ◽  
Guanine Quartet

scholarly journals Quantifying the binding stoichiometry and affinity of histo-blood group antigen oligosaccharides for human noroviruses

Glycobiology ◽  
2018 ◽  
Vol 28 (7) ◽  
pp. 488-498 ◽  
Author(s):  
Ling Han ◽  
Ruixiang Zheng ◽  
Michele R Richards ◽  
Ming Tan ◽  
Elena N Kitova ◽  
...  
Keyword(s):  
Blood Group ◽  
Conformational Changes ◽  
Binding Sites ◽  
Mass Spectra ◽  
Ion Source ◽  
Ionization Mass ◽  
Blood Group Antigens ◽  
Human Noroviruses ◽  
High Concentrations

AbstractHuman noroviruses (HuNoVs) are a major cause of acute gastroenteritis. Many HuNoVs recognize histo-blood group antigens (HBGAs) as cellular receptors or attachment factors for infection. It was recently proposed that HuNoV recognition of HBGAs involves a cooperative, multistep binding mechanism that exploits both known and previously unknown glycan binding sites. In this study, binding measurements, implemented using electrospray ionization mass spectrometry (ESI-MS) were performed on homodimers of the protruding domain (P dimers) of the capsid protein of three HuNoV strains [Saga (GII.4), Vietnam 026 (GII.10) and VA387 (GII.4)] with the ethyl glycoside of the B trisaccharide (α-d-Gal-(1→3)-[α-l-Fuc-(1→2)]-β-d-Gal-OC2H5) and free B type 1 tetrasaccharide (α-d-Gal-(1→3)-[α-l-Fuc-(1→2)]-β-d-Gal-(1→3)-d-GlcNAc) in an effort to confirm the existence of new HBGA binding sites. After correcting the mass spectra for nonspecific interactions that form in ESI droplets as they evaporate to dryness, all three P dimers were found to bind a maximum of two B trisaccharides at the highest concentrations investigated. The apparent affinities measured for stepwise binding of B trisaccharide suggest positive cooperativity. Similar results were obtained for B type 1 tetrasaccharide binding to Saga P dimer. Based on these results, it is proposed that HuNoV P dimers possess only two HBGA binding sites. It is also shown that nonspecific binding corrections applied to mass spectra acquired using energetic ion source conditions that promote in-source dissociation can lead to apparent HuNoV–HBGA oligosaccharide binding stoichiometries and affinities that are artificially high. Finally, evidence that high concentrations of oligosaccharide can induce conformational changes in HuNoV P dimers is presented.

scholarly journals Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2981
Author(s):  
Lilla Pethő ◽  
Gábor Mező ◽  
Gitta Schlosser
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectra ◽  
Source Parameters ◽  
Tumor Therapy ◽  
Ion Source ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Drug Molecules ◽  
Drug Conjugates

Peptide-based small molecule drug conjugates for targeted tumor therapy are currently in the focus of intensive research. Anthracyclines, like daunomycin, are commonly used anticancer drug molecules and are also often applied in peptide-drug conjugates. However, lability of the O-glycosidic bond during electrospray ionization mass spectrometric analysis hinders the analytical characterization of the constructs. “Overprotonation” can occur if daunomycin is linked to positively charged peptide carriers, like tuftsin derivatives. In these molecules, the high number of positive charges enhances the in-source fragmentation significantly, leading to complex mass spectra composed of mainly fragment ions. Therefore, we investigated different novel tuftsin-daunomycin conjugates to find an appropriate condition for mass spectrometric detection. Our results showed that shifting the charge states to lower charges helped to keep ions intact. In this way, a clear spectrum could be obtained containing intact protonated molecules only. Shifting of the protonation states to lower charges could be achieved with the use of appropriate neutral volatile buffers and with tuning the ion source parameters.

Reduction Reactions in the Ion Source in Electron Ionization Mass Spectrometry

2020 ◽  
Vol 75 (13) ◽  
pp. 1685-1692
Author(s):  
D. M. Mazur ◽  
M. E. Zimens ◽  
Th. B. Latkin ◽  
N. V. Ul’yanovskii ◽  
V. B. Artaev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Source ◽  
Electron Ionization ◽  
Electron Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Reduction Reactions

scholarly journals Cold‐spray ionization mass spectrometry of the choline chloride‐urea deep eutectic solvent (reline)

2021 ◽  
Vol 56 (5) ◽  
Author(s):  
Lucie Percevault ◽  
Thomas Delhaye ◽  
Alain Chaumont ◽  
Rachel Schurhammer ◽  
Ludovic Paquin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cold Spray ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass

scholarly journals Discrimination of structural isomers of amphetamine using carbon dioxide negative-ion chemical ionization mass spectrometry

1997 ◽  
Vol 13 (2) ◽  
pp. 151-161 ◽  
Author(s):  
Kevin B. Thurbide ◽  
C. M. Elson ◽  
P. G. Sim
Keyword(s):  
Carbon Dioxide ◽  
Mass Spectra ◽  
Chemical Ionization ◽  
Negative Ion ◽  
Ionization Mass ◽  
Structural Isomers ◽  
Mass Spectral ◽  
Negative Ion Chemical Ionization ◽  
Spectral Behaviour ◽  
Carbon Dioxide Plasma

The negative‒ion chemical ionization mass spectra of a group of structural isomers of amphetamine have been studied using carbon dioxide as the reagent gas. Characteristic and reproducible differences are observed for each member of the set implying that this technique offers a means of distinguishing among groups of amphetamine isomers. Characteristic adducts to the molecular ion are observed in the form (M–[H]+[O]) and (M–[H]+[CO2]). Descriptions of some fragments are given based on the mass spectral behaviour of a set of analogue compounds and the results of oxygen-18 labelled carbon dioxide reagent gas experiments. Contents of the carbon dioxide plasma and their impact on various analytes is also discussed.

The monomeric metaphosphate anion in negative-ion chemical-ionization mass spectra of phosphotriesters

1984 ◽  
Vol 106 (23) ◽  
pp. 6877-6883 ◽  
Author(s):  
Seymour Meyerson ◽  
Donald J. Harvan ◽  
J. Ronald Hass ◽  
Fausto Ramirez ◽  
James F. Marecek
Keyword(s):  
Mass Spectra ◽  
Chemical Ionization ◽  
Negative Ion ◽  
Ionization Mass ◽  
Negative Ion Chemical Ionization
Sign in / Sign up

Export Citation Format

Share Document