Singlet oxygen-induced signaling effects in mammalian cells

2003 ◽  
Vol 2 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Lars-Oliver Klotz ◽  
Klaus-Dietrich Kröncke ◽  
Helmut Sies
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells

Control of singlet oxygen production in experiments performed on single mammalian cells

2016 ◽  
Vol 321 ◽  
pp. 297-308 ◽  
Author(s):  
Michael Westberg ◽  
Mikkel Bregnhøj ◽  
Alfonso Blázquez-Castro ◽  
Thomas Breitenbach ◽  
Michael Etzerodt ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Oxygen Production

scholarly journals Singlet Oxygen induced mutation spectrum in mammalian cells

1992 ◽  
Vol 20 (16) ◽  
pp. 4319-4323 ◽  
Author(s):  
Reginas Costa de Biologia ◽  
Denise T. Riberio ◽  
Rogerio G. Nigro ◽  
Paolo Di Mascio ◽  
Carlos F.M. Menck
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Mutation Spectrum ◽  
Induced Mutation

Antioxidant β-Carotene Does Not Quench Singlet Oxygen in Mammalian Cells

2012 ◽  
Vol 135 (1) ◽  
pp. 272-279 ◽  
Author(s):  
Gabriela N. Bosio ◽  
Thomas Breitenbach ◽  
Julieta Parisi ◽  
Miguel Reigosa ◽  
Frances H. Blaikie ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Quench Singlet Oxygen ◽  
Β Carotene

Singlet oxygen-mediated damage to cellular DNA determined by the comet assay associated with DNA repair enzymes

2004 ◽  
Vol 385 (1) ◽  
pp. 17-20 ◽  
Author(s):  
J.-L. Ravanat ◽  
S. Sauvaigo ◽  
S. Caillat ◽  
G. R. Martinez ◽  
M. H. G. Medeiros ◽  
...  
Keyword(s):  
Dna Repair ◽  
Comet Assay ◽  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Singlet Molecular Oxygen ◽  
Dna Repair Enzymes ◽  
Strand Breaks ◽  
Endonuclease Iii ◽  
Repair Enzymes ◽  
Cellular Dna

Abstract The damage profile produced by the reaction of singlet molecular oxygen with cellular DNA was determined using the comet assay associated with DNA repair enzymes. Singlet oxygen was produced intracellularly by thermal decomposition of a watersoluble endoperoxide of a naphthalene derivative which is able to penetrate through the membrane into mammalian cells. We found that the DNA modifications produced by singlet oxygen were almost exclusively oxidised purines recognised by the formamidopyrimidine DNA N-glycosylase. In contrast, significant amounts of direct strand breaks and alkalilabile sites or oxidised pyrimidines, detectable by the bacterial endonuclease III, were not produced.

scholarly journals A preliminary assessment of singlet oxygen scavenging, cytotoxic and genotoxic properties of Geranium macrorrhizum extracts.

2010 ◽  
Vol 57 (2) ◽  
Author(s):  
Petras Rimantas Venskutonis ◽  
Veronika Dedonyte ◽  
Juozas Lazutka ◽  
Grazina Slapsyte ◽  
Audrone Maroziene ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Chromosome Aberration ◽  
Mammalian Cells ◽  
Human Lymphocytes ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Preliminary Assessment ◽  
Oxygen Scavenging ◽  
Catalyzed Oxidation

Strong radical-scavenging activity of Geranium macrorrhizum extracts isolated by using various solvent systems has been reported previously. This study aimed at expanding the knowledge on the bioactivities of antioxidatively active G. macrorrhizum butanol fraction, which was isolated from ethanolic extract (EB), and water fraction, which was isolated from water extract (WW) by measuring their singlet oxygen scavenging properties, as well as preliminary assessment of cytotoxicity and genotoxicity toward mammalian cells. The cytotoxicity (necrosis induction) of the extracts in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was partly prevented by antioxidants and stimulated by the prooxidant BCNU (N,N'-bis(2-chloroethyl)-N-nitrosourea). This indicates that the cytotoxicity of G. macrorrhizum extracts is at least partly attributed to their prooxidant action, presumably due to the formation of quinoidal products of their (auto)oxidation. The latter was evidenced by the nature of the peroxidase-catalyzed oxidation products, which supported DT-diaphorase-catalyzed oxidation of NADPH and participated in conjugation reactions with reduced glutathione. The genotoxic properties were studied using chromosome aberration (CA) and sister chromatid exchange (SCE) tests in human lymphocytes in vitro and Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo. In the CA test, only the highest doses of both fractions significantly increased chromosome aberration frequency. In the SCE test, both fractions induced SCEs in a clear dose-dependent manner. G. macrorrhizum extracts were not genotoxic in the SMART test in vivo. Our data indicate that in spite of the possible beneficial (antioxidant) effects of Geranium extracts, the possibilities of their use as ingredients of functional foods and/or food supplements should be further examined due to their cyto- and genotoxic effects resulting mainly from the action of quercetin-derived components abundant in the extracts.

Exerting better control and specificity with singlet oxygen experiments in live mammalian cells

Methods ◽  
2016 ◽  
Vol 109 ◽  
pp. 81-91 ◽  
Author(s):  
Michael Westberg ◽  
Mikkel Bregnhøj ◽  
Chiranjib Banerjee ◽  
Alfonso Blázquez-Castro ◽  
Thomas Breitenbach ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells

The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach

2015 ◽  
Vol 14 (4) ◽  
pp. 700-713 ◽  
Author(s):  
Marek Scholz ◽  
Anna-Louisa Biehl ◽  
Roman Dědic ◽  
Jan Hála
Keyword(s):  
Excited State ◽  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Delayed Fluorescence ◽  
Fibroblast Cells ◽  
Proof Of Concept ◽  
Time Resolved ◽  
Excited State Lifetimes ◽  
Kinetics Of

Microsecond kinetics of singlet-oxygen-sensitized delayed fluorescence (SOSDF) have been detected from individual living fibroblast cells as a proof-of-concept. These provide valuable information about excited state lifetimes and their changes during PDT-like treatment.

scholarly journals Activated Toxicity of Diesel Particulate Extract by Ultraviolet A Radiation in Mammalian Cells: Role of Singlet Oxygen

2009 ◽  
Vol 117 (3) ◽  
pp. 436-441 ◽  
Author(s):  
Lingzhi Bao ◽  
An Xu ◽  
Liping Tong ◽  
Shaopeng Chen ◽  
Lingyan Zhu ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Mammalian Cells ◽  
Ultraviolet A ◽  
Diesel Particulate ◽  
Diesel Particulate Extract

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

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