Chemiluminescence biosensor chip based on a microreactor using carrier air flow for determination of uric acid in human serum

The Analyst ◽  
2002 ◽  
Vol 127 (9) ◽  
pp. 1176-1179 ◽  
Author(s):  
Yi Lv ◽  
Zhujun Zhang ◽  
Funan Chen
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Air Flow ◽  
Biosensor Chip

scholarly journals Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

2011 ◽  
Vol 6 ◽  
pp. ACI.S7346 ◽  
Author(s):  
Ani Mulyasuryani ◽  
Arie Srihardiastutie
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Candida Utilis ◽  
Medical Laboratory ◽  
Membrane Thickness ◽  
Serum Samples ◽  
Ph Change ◽  
Pt Electrode ◽  
Enzyme Biosensor

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

scholarly journals Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent

2003 ◽  
Vol 68 (8-9) ◽  
pp. 691-698 ◽  
Author(s):  
Milena Jelikic-Stankov ◽  
Predrag Djurdjevic ◽  
Dejan Stankov
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Limit Of Detection ◽  
Ascorbate Oxidase ◽  
Acid Oxidation ◽  
Uric Acid Concentration ◽  
Enzymatic Method ◽  
Limit Of Quantification ◽  
Relative Standard

In this work a new enzymatic method for the determination of uric acid in human serum has been developed. The method is based on the oxidative coupling reaction between the N-methyl-N-(4-aminophenyl)-3-methoxyaniline (NCP) reagent and the hydrogen ? donor reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (TOOS), in the system involving three enzymes: uricase, peroxidase and ascorbate oxidase. Using this method uric acid could be determined in concentrations up to 1.428 mmol/L, with a relative standard deviation of up to 1.8 %. The effect of the medium pH and the NCP concentration on the linearity of the chromogen absorbance versus the uric acid concentration curve was investigated. The influence of the uricase activity on the maximum rate of uric acid oxidation was also examined. The use of the NCP reagent demonstrated a more precise and more sensitive determination of the uric acid compared to the determination with 4-aminoantipyrine (4-AA) as the coupling regent. The sensitivity of the method determined from the calibration curve was 0.71 absorbance units per mmol/L of uric acid; the limit of detection was LOD = 0.0035 mmol/L and the limit of quantification was LOQ = 0.015 mmol/L of uric acid.

High polarity analytes in biological matrix: determination of urea and uric acid in human serum (CCQM-K109)

Metrologia ◽  
2019 ◽  
Vol 56 (1A) ◽  
pp. 08006-08006
Author(s):  
Qinde Liu ◽  
Hong Liu ◽  
Yizhao Chen ◽  
Sharon Yong ◽  
Hui Ling Teo ◽  
...  
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Biological Matrix ◽  
High Polarity

Spectrofluorimetric method for the determination of uric acid in human serum

2014 ◽  
Vol 69 (5) ◽  
pp. 480-484 ◽  
Author(s):  
W. W. Bian ◽  
L. Wang ◽  
N. Zhang ◽  
C. Q. Jiang
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Spectrofluorimetric Method

scholarly journals Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Zhenghua Song
Keyword(s):  
Uric Acid ◽  
Food Intake ◽  
Human Serum ◽  
Flow Injection ◽  
Human Urine ◽  
Inhibitory Effect ◽  
System A ◽  
Flow Injection Chemiluminescence ◽  
Sensitive Method

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

A New Spectrofluorimetric Method for the Determination of Uric Acid in Human Serum

2014 ◽  
Vol 69 (5) ◽  
pp. 529-533
Author(s):  
W. W. Bian ◽  
L. Wang ◽  
N. Zhang ◽  
C. Q. Jiang
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Spectrofluorimetric Method
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