Supramolecular assemblies of photochromic benzodithia-18-crown-6 ethers in crystals, solutions, and monolayersElectronic supplementary information (ESI) available: crystal data, data collection, and structure solution and refinement parameters. See http://www.rsc.org/suppdata/nj/b1/b110630a/

2002 ◽  
Vol 26 (5) ◽  
pp. 543-553 ◽  
Author(s):  
Olga A. Fedorova ◽  
Yuri V. Fedorov ◽  
Artem I. Vedernikov ◽  
Olga V. Yescheulova ◽  
Sergei P. Gromov ◽  
...  
Keyword(s):  
Data Collection ◽  
Supramolecular Assemblies ◽  
Supplementary Information ◽  
Crystal Data ◽  
Structure Solution

Stationary Crystal Diffraction with a Monochromatic Convergent X-ray Source and Application for Macromolecular Crystal Data Collection

1998 ◽  
Vol 54 (2) ◽  
pp. 200-214 ◽  
Author(s):  
Joseph X. Ho ◽  
Eddie H. Snell ◽  
R. Charles Sisk ◽  
John R. Ruble ◽  
Daniel C. Carter ◽  
...  
Keyword(s):  
Data Collection ◽  
Crystal Data ◽  
X Ray

scholarly journals Collection of continuous rotation MicroED Data from Ion Beam Milled Crystals of Any Size

2018 ◽  
Author(s):  
Michael W. Martynowycz ◽  
Wei Zhao ◽  
Johan Hattne ◽  
Grant J. Jensen ◽  
Tamir Gonen
Keyword(s):  
Data Collection ◽  
Sample Preparation ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Air Interface ◽  
Structure Solution ◽  
Continuous Rotation ◽  
Macromolecular Protein ◽  
Subsequent Sample

AbstractMicrocrystal electron diffraction (MicroED) allows for macromolecular structure solution from nanocrystals. To create crystals of suitable size for MicroED data collection, sample preparation typically involves sonication or pipetting a slurry of crystals from a crystallization drop. The resultant crystal fragments are fragile and the quality of the data that can be obtained from them is sensitive to subsequent sample preparation for cryoEM as interactions in the water-air interface can damage crystals during blotting. Here, we demonstrate the use of a focused ion beam to generate lamellae of macromolecular protein crystals for continuous rotation MicroED that are of ideal thickness, easy to locate, and require no blotting optimization. In this manner, crystals of nearly any size may be scooped and milled to ideal dimensions prior to data collection, thus streamlining the methodology for sample preparation for MicroED.

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