Unstable trivalent arsenic metabolites, monomethylarsonous acid and dimethylarsinous acid

2001 ◽  
Vol 16 (12) ◽  
pp. 1409-1413 ◽  
Author(s):  
Zhilong Gong ◽  
Xiufen Lu ◽  
William R. Cullen ◽  
X. Chris Le
Keyword(s):  
Trivalent Arsenic ◽  
Monomethylarsonous Acid ◽  
Dimethylarsinous Acid ◽  
Arsenic Metabolites

scholarly journals Methylated Trivalent Arsenic-Glutathione Complexes are More Stable than their Arsenite Analog

2008 ◽  
Vol 2008 ◽  
pp. 1-8 ◽  
Author(s):  
Andrew J. Percy ◽  
Jürgen Gailer
Keyword(s):  
Arsenic Species ◽  
Size Exclusion ◽  
Trivalent Arsenic ◽  
Physiological Conditions ◽  
Column Formation ◽  
Monomethylarsonous Acid ◽  
Exclusion Chromatography ◽  
Dimethylarsinous Acid ◽  
Phosphate Buffered Saline ◽  
Comparative Stability

The trivalent arsenic glutathione complexes arsenic triglutathione, methylarsonous diglutathione, and dimethylarsinous glutathione are key intermediates in the mammalian metabolism of arsenite and possibly represent the arsenic species that are transported from the liver to the kidney for urinary excretion. Despite this, the comparative stability of the arsenic-sulfur bonds in these complexes has not been investigated under physiological conditions resembling hepatocyte cytosol. Using size-exclusion chromatography and a glutathione-containing phosphate buffered saline mobile phase (5 or 10 mM glutathione, pH 7.4) in conjunction with an arsenic-specific detector, we chromatographed arsenite, monomethylarsonous acid, and dimethylarsinous acid. The on-column formation of the corresponding arsenic-glutathione complexes between 4 and37°C revealed that methylated arsenic-glutathione complexes are more stable than arsenic triglutathione. The relevance of these results with regard to the metabolic fate of arsenite in mammals is discussed.

Trivalent methylated arsenic metabolites induce apoptosis in human myeloid leukemic HL-60 cells through generation of reactive oxygen species

Metallomics ◽  
2014 ◽  
Vol 6 (8) ◽  
pp. 1502-1512 ◽  
Author(s):  
Kanwal Rehman ◽  
Yu Jie Fu ◽  
Yan Fang Zhang ◽  
Qian Qian Wang ◽  
Bin Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Apoptosis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Trivalent Arsenic ◽  
Arsenic Metabolites

Trivalent arsenic metabolites mediate HL-60 cell apoptosis via ROS.

scholarly journals Direct analysis and stability of methylated trivalent arsenic metabolites in cells and tissues

Metallomics ◽  
2011 ◽  
Vol 3 (12) ◽  
pp. 1347 ◽  
Author(s):  
Jenna M. Currier ◽  
Milan Svoboda ◽  
Tomáš Matoušek ◽  
Jiří Dědina ◽  
Miroslav Stýblo
Keyword(s):  
Direct Analysis ◽  
Trivalent Arsenic ◽  
Arsenic Metabolites ◽  
In Cells

scholarly journals Arsenic Metabolites in Human Urine after Ingestion of an Arsenosugar

2002 ◽  
Vol 48 (1) ◽  
pp. 92-101 ◽  
Author(s):  
Kevin A Francesconi ◽  
René Tanggaar ◽  
Christine J McKenzie ◽  
Walter Goessler
Keyword(s):  
Mass Spectrometry ◽  
Human Urine ◽  
Inductively Coupled Plasma ◽  
Ionization Mass ◽  
Inductively Coupled ◽  
Urinary Arsenic ◽  
Arsenic Metabolite ◽  
Plasma Mass Spectrometry ◽  
Dimethylarsinous Acid ◽  
Arsenic Metabolites

Abstract Background: Arsenic-containing carbohydrates (arsenosugars) are common constituents of marine algae, including those species used as human food. The toxicology of these compounds has not been fully evaluated. Methods: Arsenic metabolites in human urine were monitored over a 4-day period after ingestion of a synthetic specimen of arsenosugar. The metabolites were determined by HPLC-inductively coupled plasma mass spectrometry, and structural assignments were confirmed with liquid chromatography-electrospray ionization mass spectrometry. Results: Approximately 80% of the total ingested arsenic was excreted in the urine during the 4 days of the experiment. There was a lag-period of ∼13 h before substantial quantities of arsenic appeared in the urine, and the excretion rate peaked between 22 and 31 h. At least 12 arsenic metabolites were detected, only 3 of which could be positively identified. Dimethylarsinate (DMA) was the major metabolite, constituting 67% of the total arsenicals excreted. A new urinary arsenic metabolite, dimethylarsinoylethanol, represented 5% of the total arsenicals, whereas trimethylarsine oxide was present as a trace (0.5%) constituent. One other significant metabolite cochromatographed with a reduced DMA standard, and hence was possibly dimethylarsinous acid. The second most abundant metabolite in the urine (20% of the total arsenic) remained unidentified, whereas the rest of the excreted arsenic was made up of several trace metabolites and small amounts of unchanged arsenosugar. Conclusions: Arsenosugars are biotransformed by humans to at least 12 arsenic metabolites, the toxicologies of which are currently unknown.

scholarly journals Exposure to monomethylarsonous acid (MMAIII) leads to altered selenoprotein synthesis in a primary human lung cell model

2009 ◽  
Vol 239 (2) ◽  
pp. 130-136 ◽  
Author(s):  
Sarah R. Meno ◽  
Rebecca Nelson ◽  
Korry J. Hintze ◽  
William T. Self
Keyword(s):  
Human Lung ◽  
Cell Model ◽  
Monomethylarsonous Acid
Sign in / Sign up

Export Citation Format

Share Document