A novel class of cationic gemini surfactants showing efficient in vitro gene transfection properties

2000 ◽  
pp. 1253-1254 ◽  
Author(s):  
Patrick Camilleri ◽  
Andreas Kremer ◽  
Andrew J. Edwards ◽  
Kevin H. Jennings ◽  
Owen Jenkins ◽  
...  
Keyword(s):  
Gemini Surfactants ◽  
Gene Transfection ◽  
Cationic Gemini Surfactants

scholarly journals Development of Lyophilized Gemini Surfactant-Based Gene Delivery Systems: Influence of Lyophilization on the Structure, Activity and Stability of the Lipoplexes

2012 ◽  
Vol 15 (4) ◽  
pp. 548 ◽  
Author(s):  
Waleed Mohammed-Saeid ◽  
Deborah Michel ◽  
Anas El-Aneed ◽  
Ronald E Verrall ◽  
Nicholas H Low ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Gemini Surfactant ◽  
Gemini Surfactants ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Physiochemical Properties ◽  
Cationic Gemini Surfactants ◽  
The Stability ◽  
In Vitro Transfection

Purpose. Cationic gemini surfactants have been studied as non-viral vectors for gene therapy. Clinical applications of cationic lipid/DNA lipoplexes are restricted by their instability in aqueous formulations. In this work, we investigated the influence of lyophilization on the essential physiochemical properties and in vitro transfection of gemini surfactant-lipoplexes. Additionally, we evaluated the feasibility of lyophilization as a technique for preparing lipoplexes with long term stability. Methods. A gemini surfactant [12-7NH-12] and plasmid DNA encoding for interferon-γ were used to prepare gemini surfactant/pDNA [P/G] lipoplexes. Helper lipid DOPE [L] was incorporated in all formulation producing a [P/G/L] system. Sucrose and trehalose were utilized as stabilizing agents. To evaluate the ability of lyophilization to improve the stability of gemini surfactant-based lipoplexes, four lyophilized formulations were stored at 25˚C for three months. The formulations were analyzed at different time-points for physiochemical properties and in vitro transfection. Results. The results showed that both sucrose and trehalose provided anticipated stabilizing effect. The transfection efficiency of the lipoplexes increased 2-3 fold compared to fresh formulations upon lyophilization. This effect can be attributed to the improvement of DNA compaction and changes in the lipoplex morphology due to the lyophilization/rehydration cycles. The physiochemical properties of the lyophilized formulations were maintained throughout the stability study. All lyophilized formulations showed a significant loss of gene transfection activity after three months of storage. Nevertheless, no significant losses of transfection efficiency were observed for three formulations after two months storage at 25 ˚C. Conclusion. Lyophilization significantly improved the physical stability of gemini surfactant-based lipoplexes compared to liquid formulations. As well, lyophilization improved the transfection efficiency of the lipoplexes. The loss of transfection activity upon storage is most probably due to the conformational changes in the supramolecular structure of the lipoplexes as a function of time and temperature rather than to DNA degradation. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Aggregation Properties of a Novel Class of Cationic Gemini Surfactants Correlate with Their Efficiency as Gene Transfection Agents

Langmuir ◽  
2002 ◽  
Vol 18 (6) ◽  
pp. 2426-2429 ◽  
Author(s):  
Kevin H. Jennings ◽  
Ian C. B. Marshall ◽  
Michael J. Wilkinson ◽  
Andreas Kremer ◽  
Anthony J. Kirby ◽  
...  
Keyword(s):  
Gemini Surfactants ◽  
Gene Transfection ◽  
Cationic Gemini Surfactants

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

Hydrophobized dextran-spermine conjugate as potential vector for in vitro gene transfection

2004 ◽  
Vol 96 (2) ◽  
pp. 309-323 ◽  
Author(s):  
Tony Azzam ◽  
Hagit Eliyahu ◽  
Arik Makovitzki ◽  
Michal Linial ◽  
Abraham J Domb
Keyword(s):  
Gene Transfection ◽  
Potential Vector

Mixing Behavior of Anionic Hydrotropes with Cationic Gemini Surfactants

2010 ◽  
Vol 55 (11) ◽  
pp. 4775-4779 ◽  
Author(s):  
Iqrar Ahmad Khan ◽  
Ahmad Jahan Khanam ◽  
Ziya Ahmad Khan ◽  
Kabir-ud-Din
Keyword(s):  
Gemini Surfactants ◽  
Cationic Gemini Surfactants ◽  
Mixing Behavior

Surface Tension and Aggregation Properties of Novel Cationic Gemini Surfactants with Diethylammonium Headgroups and a Diamido Spacer

Langmuir ◽  
2012 ◽  
Vol 28 (33) ◽  
pp. 11979-11987 ◽  
Author(s):  
Qi Zhang ◽  
Zhinong Gao ◽  
Feng Xu ◽  
Shuxin Tai ◽  
Xueguo Liu ◽  
...  
Keyword(s):  
Surface Tension ◽  
Gemini Surfactants ◽  
Cationic Gemini Surfactants
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