scholarly journals The solution structure of supramolecular lanthanide triple helices revisited: application of crystal-field independent paramagnetic NMR techniques to mono- and di-metallic complexes†

2000 ◽  
pp. 2045-2053 ◽  
Author(s):  
Stéphane Rigault ◽  
Claude Piguet ◽  
Jean-Claude G. Bünzli
Keyword(s):  
Crystal Field ◽  
Solution Structure ◽  
Paramagnetic Nmr ◽  
Field Independent ◽  
Nmr Techniques ◽  
Triple Helices

The crystal-field-independent transitions in orthoaxial mniicomplexes.

1973 ◽  
Vol 19 ◽  
pp. 709-717 ◽  
Author(s):  
Jacek Karwowoski ◽  
Alicja Lodzinska
Keyword(s):  
Crystal Field ◽  
Field Independent

scholarly journals Human T-lymphotrophic virus type I nucleocapsid protein NCp15: structural study and stability of the N-terminal zinc-finger

2000 ◽  
Vol 352 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Françoise BERTOLA ◽  
Claude MANIGAND ◽  
Philippe PICARD ◽  
Maya BELGHAZI ◽  
Gilles PRECIGOUX
Keyword(s):  
Zinc Finger ◽  
Virus Type ◽  
Solution Structure ◽  
Solid Phase ◽  
Original Method ◽  
Phase Method ◽  
Type I ◽  
Solid Phase Method ◽  
Nmr Techniques ◽  
The Stability

An 18-residue peptide, corresponding to the minimum sequence of the N-terminal zinc-finger domain in the nucleocapsid of human T-lymphotrophic virus type I, was synthesized by a solid-phase method and fully characterized. Its ability to complex metal ions (Co2+ and Zn2+) was clearly established by UV–visible spectroscopy and MS. The stability of these complexes was investigated by an original method with HPLC chromatography. Our results show that, even in the presence of air, the Zn2+ complex is highly stable. In contrast, the Co2+ complex undergoes a relatively fast degradation due to an intramolecular oxidation leading to the formation of a disulphide bridge between two cysteine residues. The 1H-NMR analysis indicates that Zn2+ binds to the Nδ atom of the histidine residue rather than to the Nε atom. Two-dimensional NMR techniques were used to determine the solution structure of the zinc-finger, illustrated by the existence of turns in the overall conformation.

Effect of magnetic anisotropy on direct chiral discrimination in paramagnetic NMR spectroscopy

2020 ◽  
Vol 22 (16) ◽  
pp. 8427-8441
Author(s):  
Simone Calvello ◽  
Alessandro Soncini
Keyword(s):  
Nmr Spectroscopy ◽  
Magnetic Anisotropy ◽  
Crystal Field ◽  
Room Temperature ◽  
Chiral Discrimination ◽  
Paramagnetic Nmr

We have studied the effect of thermally populated crystal field states on room temperature chiral discrimination in NMR spectroscopy.

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