Photoluminescence of pyrenebutyric acid incorporated into silicone film as a technique in luminescent oxygen sensing

The Analyst ◽  
1995 ◽  
Vol 120 (6) ◽  
pp. 1633 ◽  
Author(s):  
Toru Ishiji ◽  
Masao Kaneko
Keyword(s):  
Oxygen Sensing ◽  
Silicone Film

scholarly journals Difluoroboron β-diketonate polylactic acid oxygen nanosensors for intracellular neuronal imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Meng Zhuang ◽  
Suchitra Joshi ◽  
Huayu Sun ◽  
Tamal Batabyal ◽  
Cassandra L. Fraser ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Oxygen Sensing ◽  
Brain Slices ◽  
Neuronal Cell ◽  
Quantum Yields ◽  
Poly Lactic Acid ◽  
Neuronal Cultures ◽  
Primary Neuronal Cultures ◽  
Non Invasive ◽  
Mitotracker Red

AbstractCritical for metabolism, oxygen plays an essential role in maintaining the structure and function of neurons. Oxygen sensing is important in common neurological disorders such as strokes, seizures, or neonatal hypoxic–ischemic injuries, which result from an imbalance between metabolic demand and oxygen supply. Phosphorescence quenching by oxygen provides a non-invasive optical method to measure oxygen levels within cells and tissues. Difluoroboron β-diketonates are a family of luminophores with high quantum yields and tunable fluorescence and phosphorescence when embedded in certain rigid matrices such as poly (lactic acid) (PLA). Boron nanoparticles (BNPs) can be fabricated from dye-PLA materials for oxygen mapping in a variety of biological milieu. These dual-emissive nanoparticles have oxygen-insensitive fluorescence, oxygen-sensitive phosphorescence, and rigid matrix all in one, enabling real-time ratiometric oxygen sensing at micron-level spatial and millisecond-level temporal resolution. In this study, BNPs are applied in mouse brain slices to investigate oxygen distributions and neuronal activity. The optical properties and physical stability of BNPs in a biologically relevant buffer were stable. Primary neuronal cultures were labeled by BNPs and the mitochondria membrane probe MitoTracker Red FM. BNPs were taken up by neuronal cell bodies, at dendrites, and at synapses, and the localization of BNPs was consistent with that of MitoTracker Red FM. The brain slices were stained with the BNPs, and the BNPs did not significantly affect the electrophysiological properties of neurons. Oxygen maps were generated in living brain slices where oxygen is found to be mostly consumed by mitochondria near synapses. Finally, the BNPs exhibited excellent response when the conditions varied from normoxic to hypoxic and when the neuronal activity was increased by increasing K+ concentration. This work demonstrates the capability of BNPs as a non-invasive tool in oxygen sensing and could provide fundamental insight into neuronal mechanisms and excitability research.

Oxygen sensing in crustaceans: functions and mechanisms

2021 ◽  
Author(s):  
Tábata Martins de Lima ◽  
Luiz Eduardo Maia Nery ◽  
Fábio Everton Maciel ◽  
Hanh Ngo-Vu ◽  
Mihika T. Kozma ◽  
...  
Keyword(s):  
Oxygen Sensing

scholarly journals To breathe or not to breathe: Understanding how oxygen sensing contributes to age-related phenotypes

2021 ◽  
Vol 67 ◽  
pp. 101267
Author(s):  
T. van Vliet ◽  
F. Casciaro ◽  
M. Demaria
Keyword(s):  
Oxygen Sensing ◽  
Age Related

Titanate-PMMA composites in photoluminescence based oxygen sensing

2021 ◽  
Vol 133 ◽  
pp. 105942
Author(s):  
O. Marantos ◽  
V. Binas ◽  
M. Moschogiannaki ◽  
E. Gagaoudakis ◽  
G. Kiriakidis ◽  
...  
Keyword(s):  
Oxygen Sensing
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