Use of chemometric factor analysis for chromatographic integration: application to diode-array high-performance liquid chromatography of mixtures of chlorophyll a degradation products

The Analyst ◽  
1993 ◽  
Vol 118 (7) ◽  
pp. 779 ◽  
Author(s):  
Yi-Zeng Liang ◽  
Richard G. Brereton ◽  
Olav M. Kvalheim ◽  
Ali Rahmani
Keyword(s):  
Factor Analysis ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chlorophyll A ◽  
High Performance ◽  
Degradation Products ◽  
Diode Array

A Simple Method for the Determination of Carbaryl and 1-Naphthol in Fruit Juices by High-Performance Liquid Chromatography–Diode-Array Detection

2003 ◽  
Vol 66 (8) ◽  
pp. 1510-1513 ◽  
Author(s):  
GÜL ÖZHAN ◽  
SIBEL TOPUZ ◽  
BUKET ALPERTUNGA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fruit Juice ◽  
Degradation Products ◽  
Diode Array ◽  
Diode Array Detection ◽  
Simple Method ◽  
Array Detection

Carbaryl (Sevin) is a widely used N-methylcarbamate insecticide. In this study, a simple and sensitive reversed-phase, high-performance liquid chromatography method with diode-array detection has been developed for the determination of low levels of carbaryl and its degradation products 1-naphthol in several kinds of canned pure fruit juice. The compounds were captured on a C18 cartridge. The analytes were separated on a C18 column using a linear gradient of 40 to 60% acetonitrile in water in a period of 20 min. The extraction recoveries of carbaryl and 1-naphthol were in the range 93.5 to 98.0% and 90.7 to 96.0% for fruit juice, respectively. The detection limit was below 0.8 ng/ml and the calibration curves showed good linearity between 0.9998 and 0.9999.

Chlorophyll a extraction from freshwater algae — a reevaluation

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Michael Schagerl ◽  
Gabriela Künzl
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chlorophyll A ◽  
High Performance ◽  
Degradation Products ◽  
Cell Disruption ◽  
Freshwater Algae ◽  
Freshwater Phytoplankton

AbstractIn this study, two extracting methods (sonication and dispersing) and three solvents (90% acetone, N,N′-dimethylformamide and methanol) were compared for their ability to extract chlorophyll a of freshwater phytoplankton. Measurements were performed with both spectrophotometry and high-performance liquid chromatography. Results showed that (i) cell disruption is essential and that (ii) the method of cell disruption and solvent applied differed significantly. Dispersing in acetone surpassed all other combinations. Sonication in N,N′-dimethylformamide was found less effective. N,N′-dimethylformamide and methanol seem to promote the formation of degradation products (chlorophyllide a, allomer, epimer and phaeophytin a) which lead to overestimates of chlorophyll a of about 10% by means of spectrophotometry.

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