Modification of the high-performance liquid chromatographic retention behaviour of β-diketones by the inclusion of metal ions in the mobile phase

The Analyst ◽  
1990 ◽  
Vol 115 (12) ◽  
pp. 1517-1523 ◽  
Author(s):  
Roger M. Smith ◽  
Simon J. Bale ◽  
Stephen G. Westcott ◽  
M. Martin-Smith
Keyword(s):  
Metal Ions ◽  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Retention ◽  
Retention Behaviour ◽  
Liquid Chromatographic

Analysis of Sucrose Esters by HPLC Using Charged Aerosol Detector

2012 ◽  
Vol 554-556 ◽  
pp. 1962-1966 ◽  
Author(s):  
Xiao Wen Miao ◽  
Zhi Dong Chang ◽  
Wen Jun Li ◽  
Rong Rong Zhao ◽  
Bin Dong ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Sucrose Esters ◽  
Phase Compensation ◽  
Charged Aerosol ◽  
Liquid Chromatographic Method ◽  
Gradient Composition ◽  
C 30 ◽  
Liquid Chromatographic

A high performance liquid chromatographic method has been developed for separation and quantitation of sucrose esters using charged aerosol detection (CAD) combined with mobile phase compensation. Two Acclaim120 C18 columns (75×3.0mm, 3μm) and the gradient composition (0 min – 72% A + 25% B + 3%C, 7.5 min – 75% A + 25% B + 3%C, 19.5 min – 97% A + 3%C, 30 min – 97% A + 3%C, where A is methanol, B is water and C is tetrahydrofuran) were applied. A precisely inverse gradient composition (0 min – 97% A + 3%C, 7.5 min – 97% A + 3%C, 19.5 min – 72% A + 25% B + 3%C, 30 min – 72% A + 25% B + 3%C) was also used. The mobile phase compensation was performed by mixing of the column effluent with the mobile phase of exactly reverse composition provided by a second pump before introduction into the CAD. Introduction

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