Improved determination of hydrogen peroxide or lucigenin by measurement of lucigenin chemiluminescence in organised assemblies

The Analyst ◽  
1986 ◽  
Vol 111 (8) ◽  
pp. 941 ◽  
Author(s):  
Cheryl L. Malehorn ◽  
Terrence E. Riehl ◽  
Willie L. Hinze
Keyword(s):  
Hydrogen Peroxide ◽  
Lucigenin Chemiluminescence

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

scholarly journals Determination of Hydrogen Peroxide with Peroxidase Adsorbed Carbon Electrode

1982 ◽  
Vol 102 (12) ◽  
pp. 1120-1123 ◽  
Author(s):  
HIROYUKI IWAI ◽  
SUMIYUKI AKIHAMA
Keyword(s):  
Hydrogen Peroxide ◽  
Carbon Electrode ◽  
Adsorbed Carbon
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