Improved synthesis of polymethoxytriphenyl-methanols for use as one-colour pH indicators

The Analyst ◽  
1981 ◽  
Vol 106 (1266) ◽  
pp. 1013 ◽  
Author(s):  
Jack L. Lambert ◽  
Todd C. Bird ◽  
Michael D. Rogers ◽  
Joseph V. Paukstelis
Keyword(s):  
Ph Indicators

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

Reusable glass-bound pH indicators

1975 ◽  
Vol 47 (2) ◽  
pp. 348-351 ◽  
Author(s):  
G. Bruce. Harper
Keyword(s):  
Ph Indicators

Cellulose Acetate-Based pH Indicator Optodes

1995 ◽  
Vol 48 (6) ◽  
pp. 1081 ◽  
Author(s):  
TJ Cardwell ◽  
RW Cattrall ◽  
LW Deady ◽  
M Dorkos ◽  
AJ Kaye ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Ph Indicator ◽  
Ph Indicators

Some pH indicators for use in optode membranes based on cellulose acetate have been prepared, and applications of membranes containing octyl bis (2,4-dinitrophenyl)acetate in static and flowing systems are discussed.

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