The titrimetric determination of carbon dioxide liberated in the ninhydrin reaction with amino-acids

The Analyst ◽  
1951 ◽  
Vol 76 (908) ◽  
pp. 619 ◽  
Author(s):  
A. M. Smith ◽  
A. H. Agiza
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Ninhydrin Reaction ◽  
Titrimetric Determination

Contactless, probeless and non-titrimetric determination of acid–base reactions using broadband acoustic resonance dissolution spectroscopy (BARDS)

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 956-962 ◽  
Author(s):  
M. Rizwan Ahmed ◽  
Sean McSweeney ◽  
Jacob Krüse ◽  
Bastiaan Vos ◽  
Dara Fitzpatrick
Keyword(s):  
Carbon Dioxide ◽  
Acoustic Resonance ◽  
Carbon Dioxide Production ◽  
Acid Base ◽  
Titrimetric Determination

Carbon dioxide production due to an acid–base reaction.

Influence of Cations on the Ninhydrin Reaction for the Determination of Amino-Acids

Nature ◽  
1953 ◽  
Vol 172 (4377) ◽  
pp. 543-543 ◽  
Author(s):  
HANAN MEYER ◽  
EMANUEL RIKLIS
Keyword(s):  
Amino Acids ◽  
Ninhydrin Reaction

THE QUANTITATIVE ESTIMATION OF FROST INJURY AND RESISTANCE IN BLACK LOCUST, ALFALFA, AND WHEAT TISSUES BY DETERMINATION OF AMINO ACIDS AND OTHER NINHYDRIN-REACTING SUBSTANCES RELEASED AFTER THAWING

1964 ◽  
Vol 42 (6) ◽  
pp. 637-649 ◽  
Author(s):  
D. Siminovitch ◽  
H. Therrien ◽  
F. Gfeller ◽  
B. Rheaume
Keyword(s):  
Amino Acids ◽  
Black Locust ◽  
Quantitative Estimation ◽  
Freezing Temperature ◽  
Freezing Injury ◽  
Tree Bark ◽  
Freezing Process ◽  
Frost Injury ◽  
Ninhydrin Reaction

Amino acids and other soluble α-amino compounds are liberated into a leaching medium from tissues of black locust bark, alfalfa, and wheat that are frozen to temperatures which are injurious to the tissues. The amounts liberated increase with lowering in freezing temperature and are proportional to the loss in vital capacity of the tissue. Insignificant amounts of amino acids are released by leaching of non-frozen tissue while a maximum is reached at freezing temperatures which are completely lethal. The amino acids liberated from frozen and thawed tissues must originate from the destruction of living cells by the freezing process. The determination of the concentration of amino acids in the medium in which the plant tissues are leached after freezing can be used therefore as a quantitative method for the estimation of the injury sustained in the freezing.The use of the ninhydrin reaction for the purpose of this determination is described and its application to the estimation of freezing injury and resistance in a variety of hardy and non-hardy tissues of alfalfa, wheat, and black locust tree bark is shown. The extension of this procedure to the determination of injury produced by toxic and other detrimental agents is indicated.

scholarly journals THE GASOMETRIC DETERMINATION OF AMINO ACIDS IN URINE BY THE NINHYDRIN-CARBON DIOXIDE METHOD

1943 ◽  
Vol 150 (1) ◽  
pp. 251-258 ◽  
Author(s):  
Donald D. Van Slyke ◽  
Douglas A. MacFadyen ◽  
Paul B. Hamilton
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids

A Shortened Method for the Fluorometric Determination of Phenylalanine

1969 ◽  
Vol 15 (1) ◽  
pp. 15-23 ◽  
Author(s):  
John A Ambrose
Keyword(s):  
Amino Acids ◽  
Incubation Temperature ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Reagent Blank ◽  
Ninhydrin Reaction ◽  
Fluorescence Reaction ◽  
Fluorescence Level ◽  
Ruhemann’S Purple

Abstract As a result of a study of the classic ninhydrin reaction with amino acids (which results in the production of Ruhemann’s purple), and the fluorescence reaction occurring between ninhydrin and phenylalanine in the presence of a dipeptide, the initial incubation period in the fluorescence reaction was shortened from 2 hr. to 16 min. This improvement in the fluorometric method for the determination of phenylalanine was accomplished by (1) decreasing the initial incubation pH from 5.8 to 5.0, and (2) increasing the incubation temperature from 60° to 85°. A study of the effect of pH on the fluorescence level in both the acid and alkaline reactions demonstrated the presence of fluorescence pH plateaus. These plateaus indicate that the fluorescence level was relatively unaffected by a change in pH within specified ranges. A study of the specificity led to the discovery that the inclusion of a peptide control was necessary to correct for one type of nonspecific fluorescence. To make this correction, it was necessary to add the reading of the serum blank to that of the reagent blank, and to subtract this total from the sample reading.

Sign in / Sign up

Export Citation Format

Share Document