Design, synthesis and metal binding properties of a mixed-donor macrobicycle

1998 ◽  
pp. 355-356 ◽  
Author(s):  
Mark Mascal ◽  
Jens Hansen
Keyword(s):  
Metal Binding ◽  
Binding Properties ◽  
Design Synthesis

scholarly journals Unravelling the Structure of the Tetrahedral Metal-Binding Site in METP3 through an Experimental and Computational Approach

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5221
Author(s):  
Salvatore La Gatta ◽  
Linda Leone ◽  
Ornella Maglio ◽  
Maria De Fenza ◽  
Flavia Nastri ◽  
...  
Keyword(s):  
Metal Ions ◽  
Binding Site ◽  
Metal Binding ◽  
Binding Sites ◽  
Metal Ion ◽  
Structural Determinants ◽  
Binding Properties ◽  
Tetrahedral Geometry ◽  
Design Synthesis ◽  
Metal Binding Sites

Understanding the structural determinants for metal ion coordination in metalloproteins is a fundamental issue for designing metal binding sites with predetermined geometry and activity. In order to achieve this, we report in this paper the design, synthesis and metal binding properties of METP3, a homodimer made up of a small peptide, which self assembles in the presence of tetrahedrally coordinating metal ions. METP3 was obtained through a redesign approach, starting from the previously developed METP molecule. The undecapeptide sequence of METP, which dimerizes to house a Cys4 tetrahedral binding site, was redesigned in order to accommodate a Cys2His2 site. The binding properties of METP3 were determined toward different metal ions. Successful assembly of METP3 with Co(II), Zn(II) and Cd(II), in the expected 2:1 stoichiometry and tetrahedral geometry was proven by UV-visible spectroscopy. CD measurements on both the free and metal-bound forms revealed that the metal coordination drives the peptide chain to fold into a turned conformation. Finally, NMR data of the Zn(II)-METP3 complex, together with a retrostructural analysis of the Cys-X-X-His motif in metalloproteins, allowed us to define the model structure. All the results establish the suitability of the short METP sequence for accommodating tetrahedral metal binding sites, regardless of the first coordination ligands.

ChemInform Abstract: Design, Synthesis and Metal Binding Properties of a Mixed-Donor Macrobicycle.

ChemInform ◽  
2010 ◽  
Vol 29 (19) ◽  
pp. no-no
Author(s):  
M. MASCAL ◽  
J. HANSEN ◽  
A. J. BLAKE ◽  
W.-S. LI
Keyword(s):  
Metal Binding ◽  
Binding Properties ◽  
Design Synthesis

A new family of fluorescent calcium indicators designed to resist leakage or for measuring calcium near membranes

1994 ◽  
Vol 52 ◽  
pp. 168-169
Author(s):  
Martin Poenie ◽  
Akwasi Minta ◽  
Charles Vorndran
Keyword(s):  
Metal Binding ◽  
Acetoxymethyl Ester ◽  
Binding Properties ◽  
Fura 2 ◽  
New Family ◽  
Anion Transporter ◽  
Calcium Indicator ◽  
Calcium Indicators ◽  
High Calcium ◽  
Intracellular Compartmentalization

The use of fura-2 as an intracellular calcium indicator is complicated by problems of rapid dye leakage and intracellular compartmentalization which is due to a probenecid sensitive anion transporter. In addition there is increasing evidence for localized microdomains of high calcium signals which may not be faithfully reported by fura-2.We have developed a new family of fura-2 analogs aimed at addressing some of these problems. These new indicators are based on a modified bapta which can be readily derivatized to produce fura-2 analogs with a variety of new properties. The modifications do not affect the chromophore and have little impact on the spectral and metal binding properties of the indicator. One of these new derivatives known as FPE3 is a zwitterionic analog of fura-2 that can be loaded into cells as an acetoxymethyl ester and whose retention in cells is much improved. The improved retention of FPE3 is important for both cuvettebased measurements of cell suspensions and for calcium imaging.

Rational Design, Synthesis, and DNA Binding Properties of Novel Sequence-Selective Peptidyl Congeners of Ametantrone

ChemMedChem ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. 1080-1091 ◽  
Author(s):  
Alessandra Gianoncelli ◽  
Serena Basili ◽  
Matteo Scalabrin ◽  
Alice Sosic ◽  
Stefano Moro ◽  
...  
Keyword(s):  
Dna Binding ◽  
Rational Design ◽  
Binding Properties ◽  
Design Synthesis ◽  
Dna Binding Properties

Synthesis and Metal-Binding Properties of Chelating Fluorescein Derivatives

2003 ◽  
Vol 5 (12) ◽  
pp. 2051-2054 ◽  
Author(s):  
Matthew A. Clark ◽  
Kathryn Duffy ◽  
Jyoti Tibrewala ◽  
Stephen J. Lippard
Keyword(s):  
Metal Binding ◽  
Binding Properties

Synthesis and metal binding properties of the novel ligand N,N′N″-tris(2-hydroxyethyl)-1,4,7-triazacyclononane

1983 ◽  
Vol 77 ◽  
pp. L63-L64 ◽  
Author(s):  
Bridget A. Sayer ◽  
Joseph P. Michael ◽  
Robert D. Hancock
Keyword(s):  
Metal Binding ◽  
The Novel ◽  
Binding Properties

scholarly journals Cucumber Metallothionein-Like 2 (CsMTL2) Exhibits Metal-Binding Properties

Genes ◽  
2016 ◽  
Vol 7 (12) ◽  
pp. 106 ◽  
Author(s):  
Yu Pan ◽  
Yanglu Pan ◽  
Junpeng Zhai ◽  
Yan Xiong ◽  
Jinhua Li ◽  
...  
Keyword(s):  
Metal Binding ◽  
Binding Properties

scholarly journals Expression, Purification, and Metal Binding Properties of the N-terminal Domain from the Wilson Disease Putative Copper-transporting ATPase (ATP7B)

1997 ◽  
Vol 272 (52) ◽  
pp. 33279-33282 ◽  
Author(s):  
Michael DiDonato ◽  
Suree Narindrasorasak ◽  
John R. Forbes ◽  
Diane W. Cox ◽  
Bibudhendra Sarkar
Keyword(s):  
Metal Binding ◽  
Wilson Disease ◽  
Binding Properties ◽  
Terminal Domain
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