Determination of solvent thinner components in human body fluids by capillary gas chromatography with trapping at low oven temperature for headspace samples

The Analyst ◽  
1998 ◽  
Vol 123 (1) ◽  
pp. 147-150 ◽  
Author(s):  
Xiao-Pen Lee ◽  
Takeshi Kumazawa ◽  
Keizo Sato ◽  
Takeshi Kumazawa ◽  
Kanako Watanabe ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Human Body ◽  
Capillary Gas Chromatography ◽  
Body Fluids

DETERMINATION OF CRESOL ISOMERS AND PHENOL IN HUMAN BODY FLUIDS BY CAPILLARY GAS CHROMATOGRAPHY WITH CRYOGENIC OVEN TRAPPING

2002 ◽  
Vol 35 (13) ◽  
pp. 2093-2103 ◽  
Author(s):  
Xiao-Pen Lee ◽  
Takeshi Kumazawa ◽  
Kei Saito ◽  
Megumi Takano ◽  
Hideki Hattori ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Human Body ◽  
Capillary Gas Chromatography ◽  
Body Fluids

scholarly journals Sensitive Determination of n-Hexane and Cyclohexane in Human Body Fluids by Capillary Gas Chromatography with Cryogenic Oven Trapping

2001 ◽  
Vol 84 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Kei Kondo ◽  
Xiao-Pen Lee ◽  
Takeshi Kumazawa ◽  
Keizo Sato ◽  
Kanako Watanabe-Suzuki ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Human Body ◽  
Whole Blood ◽  
Capillary Gas Chromatography ◽  
Signal To Noise Ratio ◽  
Body Fluids ◽  
Regression Equations ◽  
Flame Ionization ◽  
Glass Syringe

Abstract A sensitive method was developed for determination of n-hexane and cyclohexane in human body fluids by headspace capillary gas chromatography (GC) with cryogenic oven trapping. Whole blood and urine samples containing n-hexane and cyclohexane were heated in a 7.5 mL vial at 70°C for 15 min, and 5 mL of the headspace vapor was drawn into a glass syringe. All vapor was introduced through an injection port of a GC instrument in the splitless mode into an Rtx-Volatiles middle-bore capillary column at an oven temperature of −40°C for trapping volatile compounds. The oven temperature was programmed to 180°C for GC with flame ionization detection. These conditions gave sharp peaks for both n-hexane and cyclohexane, a good separation of each peak, and low background impurities for whole blood and urine. The extraction efficiencies of n-hexane and cyclohexane were 13.2–30.3% for whole blood and 12.7–20.7% for urine. The coefficients of within-day variation in terms of extraction efficiency of both compounds were 5.0–9.5% for whole blood and 3.8–10.8% for urine; those of day-to-day variationfor the compounds were not greater than 16.6%. The regression equations for n-hexane and cyclohexane showed good linearity in the range of 5–500 ng/0.5 mL for whole blood and urine. The detection limits (signal-to-noise ratio = 3) for both compounds were 1.2 and 0.5 ng/0.5 mL for whole blood and urine, respectively. The data on n-hexane or cyclohexane in rat blood after inhalation of each compound are also presented.

Detection of ethanol in human body fluids by headspace solid-phase micro extraction (SPME)/capillary gas chromatography

1996 ◽  
Vol 43 (7-8) ◽  
pp. 393-397 ◽  
Author(s):  
T. Kumazawa ◽  
H. Seno ◽  
X. -P. Lee ◽  
A. Ishii ◽  
O. Suzuki ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Human Body ◽  
Capillary Gas Chromatography ◽  
Solid Phase ◽  
Body Fluids ◽  
Solid Phase Micro Extraction
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