scholarly journals Iron metabolism and human ferritin heavy chain cDNA from adult brain with an elongated untranslated region: new findings and insights†

The Analyst ◽  
1998 ◽  
Vol 123 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Maire E. Percy ◽  
Simon Wong ◽  
Sharon Bauer ◽  
Negin Liaghati-Nasseri ◽  
Marc D. Perry ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Heavy Chain ◽  
Untranslated Region ◽  
Adult Brain ◽  
Ferritin Heavy Chain ◽  
New Findings ◽  
Human Ferritin ◽  
Human Ferritin Heavy Chain

Electroinduced Extraction of Human Ferritin Heavy Chain Expressed in Hansenula polymorpha

2017 ◽  
Vol 184 (4) ◽  
pp. 1286-1307 ◽  
Author(s):  
Valentina Ganeva ◽  
Bojidar Galutzov ◽  
Boyana Angelova ◽  
Manfred Suckow
Keyword(s):  
Heavy Chain ◽  
Hansenula Polymorpha ◽  
Ferritin Heavy Chain ◽  
Human Ferritin ◽  
Human Ferritin Heavy Chain

Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization

Gene ◽  
1992 ◽  
Vol 111 (2) ◽  
pp. 255-260 ◽  
Author(s):  
M.A. Bevilacqua ◽  
M. Giordano ◽  
P. D'Agostino ◽  
C. Santoro ◽  
F. Cimino ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Functional Characterization ◽  
Ferritin Heavy Chain ◽  
Human Ferritin ◽  
Encoding Gene ◽  
Human Ferritin Heavy Chain

scholarly journals Isolation and characterization of a cDNA clone for human ferritin heavy chain.

1984 ◽  
Vol 81 (15) ◽  
pp. 4751-4755 ◽  
Author(s):  
D. Boyd ◽  
S. K. Jain ◽  
J. Crampton ◽  
K. J. Barrett ◽  
J. Drysdale
Keyword(s):  
Heavy Chain ◽  
Cdna Clone ◽  
Ferritin Heavy Chain ◽  
Isolation And Characterization ◽  
Human Ferritin ◽  
Human Ferritin Heavy Chain

scholarly journals CLONING AND EXPRESSING HG-CSF (HUMAN GRANULOCYTE COLONY STIMULATING FACTOR) BEING FUSED WITH FERRITIN HEAVY-CHAIN IN E. COLI

2009 ◽  
Vol 12 (9) ◽  
pp. 47-53
Author(s):  
Hieu Thi Phuong Nguyen ◽  
Linh Thuy Lien ◽  
Thuoc Linh Tran
Keyword(s):  
Heavy Chain ◽  
Cloning And Expression ◽  
Form Expression ◽  
Gene Encoding ◽  
E Coli ◽  
Ferritin Heavy Chain ◽  
Tev Protease ◽  
Sds Page ◽  
Human Ferritin ◽  
Stimulating Factor

G-CSF is a cytokine that stimulates the proliferation, differentiation, function of mature neutrophils and is generally used for treatment of neutropenia in cancer patients under chemotherapy. In this study, we report results on the cloning and expression of hG-CSF being fused with the heavy-chain (FTN-H) of human ferritin, which had been showed to be capable of facilitate the folding of several human protein expressed in E. coli. The hg-csf gene and gene encoding FTN-H were inserted into plasmid pET28a to form expression vector PET-FHG. 6xHis tag and TEV sequence (being recognized and cleaved by TEV protease) was added between of G-CSF and FTN-H to facilitate G-CSF purification and recovery afterwards. PET-FHG was transformed into E. coli BL21(DE3). The expression of hG-CSF was induced by IPTG and confirmed by SDS-PAGE and Western blot using anti-hG-CSF antibody.

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