Circular dichroism (CD) study of peridinin[ndash ]chlorophyll a protein (PCP) complexes from marine dinoflagellate algae The tetramer approach

1998 ◽  
Vol 94 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Mariusz Pilch ◽  
Marek Pawlikowski
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Marine Dinoflagellate ◽  
Pcp Complexes ◽  
Circular Dichroïsm

scholarly journals Evidence that circularly dichroic chlorophyll forms a-682 and a-710 are oriented at right angles to the thylakoid membranes of whole chloroplasts, and that the circular dichroism is light-dependent

1975 ◽  
Vol 148 (3) ◽  
pp. 487-497 ◽  
Author(s):  
R P Gregory
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
The Other ◽  
Light Path ◽  
Preferred Direction ◽  
Polarization Of Fluorescence ◽  
Chloroplast Orientation ◽  
Circular Dichroïsm ◽  
Chlorophyll Forms ◽  
Exciton Type

Chloroplasts from the pea (Pisum sativum L.) suspended in iso-osmotic buffered medium were oriented by flow in specially constructed cuvettes and examined for circular dichroism (c.d.). In one cuvette the flow was transverse to the direction of the light-path, but the other cuvette was designed so that flow and the light-path were coaxial. The induced orientation is such that the chloroplasts appear to move edgewise. C.d. was maximum when the light-path lay in the plane of the chloroplast disc. The intense c.d. of intact chloroplasts ascribed by Gregory & Raps [Biochem. J. (1974) 142, 193-201] to bulk chlorophyll a was found to contain two components, one the split-exciton type centred at 682nm and the other a simple maximum at 700-710 nm. The chlorophyll a-710 form was distinguished by its greater dependence on chloroplast orientation. The preferred direction of the transition moment in both chlorophyll forms was at right angles to the plane of the chloroplast, that is, at right angles to the plane of the thylakoids. This is in conflict with several reports based on polarization of fluorescence. It is suggested that the present effect is due to thylakoid-thylakoid interaction. Evidence for this is the reversible diminution in the c.d. signal caused by illumination in the presence of electron-transport reagents. It is argued that the c.d. is an indicator of chlorophyll movement, or changes in the thylakoid-thylakoid distance, possibly related to ion movement, affecting energy transfer between photosynthetic units.

Circular Dichroism of Long Wavelength Forms of Chlorophyll a

Nature ◽  
1969 ◽  
Vol 224 (5224) ◽  
pp. 1108-1110 ◽  
Author(s):  
GWENDOLYN M. SHERMAN
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Long Wavelength ◽  
Circular Dichroïsm

Reversible changes in macroorganization of the light-harvesting chlorophyll a/b pigment-protein complex detected by circular dichroism

Biochemistry ◽  
1988 ◽  
Vol 27 (7) ◽  
pp. 2430-2434 ◽  
Author(s):  
Gyozo Garab ◽  
Richard C. Leegood ◽  
David A. Walker ◽  
John C. Sutherland ◽  
Geoffrey Hind
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Light Harvesting ◽  
Pigment Protein Complex ◽  
Circular Dichroïsm
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