Electron spin echo decay as a probe of aminoxyl environment in spin-labeled mutants of human carbonic anhydrase II †

1997 ◽  
pp. 2549-2554 ◽  
Author(s):  
Mikael Lindgren ◽  
Gareth R. Eaton ◽  
Sandra S. Eaton ◽  
Bengt-Harald Jonsson ◽  
Per Hammarström ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Electron Spin ◽  
Spin Echo ◽  
Electron Spin Echo ◽  
Carbonic Anhydrase Ii ◽  
Human Carbonic Anhydrase

scholarly journals The Influence of Varying Fluorination Patterns on the Thermodynamics and Kinetics of Benzenesulfonamide Binding to Human Carbonic Anhydrase II

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 509 ◽  
Author(s):  
Steffen Glöckner ◽  
Khang Ngo ◽  
Björn Wagner ◽  
Andreas Heine ◽  
Gerhard Klebe
Keyword(s):  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Ii ◽  
The Novel ◽  
Binding Modes ◽  
X Ray ◽  
Structure Activity ◽  
Small Set ◽  
Novel Method ◽  
Human Carbonic Anhydrase ◽  
Kinetics Of

The fluorination of lead-like compounds is a common tool in medicinal chemistry to alter molecular properties in various ways and with different goals. We herein present a detailed study of the binding of fluorinated benzenesulfonamides to human Carbonic Anhydrase II by complementing macromolecular X-ray crystallographic observations with thermodynamic and kinetic data collected with the novel method of kinITC. Our findings comprise so far unknown alternative binding modes in the crystalline state for some of the investigated compounds as well as complex thermodynamic and kinetic structure-activity relationships. They suggest that fluorination of the benzenesulfonamide core is especially advantageous in one position with respect to the kinetic signatures of binding and that a higher degree of fluorination does not necessarily provide for a higher affinity or more favorable kinetic binding profiles. Lastly, we propose a relationship between the kinetics of binding and ligand acidity based on a small set of compounds with similar substitution patterns.

Radio-frequency-driven electron-spin-echo-envelope-modulation spectroscopy

1991 ◽  
Vol 180 (3) ◽  
pp. 198-206 ◽  
Author(s):  
Herman Cho ◽  
Susanne Pfenninger ◽  
Jörg Forrer ◽  
Arthur Schweiger
Keyword(s):  
Radio Frequency ◽  
Electron Spin ◽  
Spin Echo ◽  
Electron Spin Echo ◽  
Modulation Spectroscopy ◽  
Envelope Modulation

Mn2+−Nitrogen Interactions in RNA Probed by Electron Spin−Echo Envelope Modulation Spectroscopy:  Application to the Hammerhead Ribozyme

1999 ◽  
Vol 121 (39) ◽  
pp. 9215-9218 ◽  
Author(s):  
Susan R. Morrissey ◽  
Thomas E. Horton ◽  
Christopher V. Grant ◽  
Charles G. Hoogstraten ◽  
R. David Britt ◽  
...  
Keyword(s):  
Electron Spin ◽  
Hammerhead Ribozyme ◽  
Spin Echo ◽  
Electron Spin Echo ◽  
Modulation Spectroscopy ◽  
Envelope Modulation
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