Use of Large Volume Sample Stacking for Improving Limits of Detection in the Capillary Electrophoretic Determination of Selected Cations

1997 ◽  
Vol 34 (1) ◽  
pp. 9-11 ◽  
Author(s):  
G. B. Harland ◽  
G. McGrath ◽  
S. McClean ◽  
W. F. Smyth
Keyword(s):  
Large Volume ◽  
Limits Of Detection ◽  
Sample Stacking ◽  
Capillary Electrophoretic ◽  
Large Volume Sample Stacking

Determination of rutin, chlorogenic acid and quercetin in solidaginis by large volume sample stacking with polarity switching and acid barrage stacking

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Chlorogenic Acid ◽  
Large Volume ◽  
Background Electrolyte ◽  
Sample Stacking ◽  
Hydrodynamic Injection ◽  
Polarity Switching ◽  
Capillary Electrophoretic ◽  
Electrolyte Ph ◽  
Large Volume Sample Stacking

The sensitivity of capillary electrophoretic separation of rutin, chlorogenic acid and quercetin was enhanced by combination use of large volume sample stacking with polarity switching (LVSSPS) and acid barrage stacking (ABS). Separating conditions, including the background electrolyte pH and concentration, sample injection and acid barrage were optimized. The optimum conditions were: a background electrolyte of 30 mM Na2B2O7 of pH 9.25, hydrodynamic injection of the sample (60s, 5 psi), then applied voltage of -25 kV, and then hydrodynamic injecting of 0.15 mol/L HAc (18 s, 0.5 psi), and at last separation with 25 kV. Under these conditions, the three analytes could be separated with a sample-to-sample time of 14 min and detection limits from 9.0 to 12.5 ng/mL. When compared to a conventional hydrodynamic injection, the sensitivity was enhanced between 333 to 506 times and the method is 3.6-5.3 times more sensitive than LVSSPS. The applicability of the developed method was demonstrated by the detection of the analytes in aqueous extract of solidaginis.

Highly sensitive detection of copper(i) and copper(ii) in cell specimens by CE-UV with large-volume sample stacking

2016 ◽  
Vol 8 (21) ◽  
pp. 4272-4276 ◽  
Author(s):  
Cheng Chen ◽  
Xin Li ◽  
Xia Xie ◽  
Fengxia Chang ◽  
Meixian Li ◽  
...  
Keyword(s):  
Large Volume ◽  
Sensitive Detection ◽  
Sample Stacking ◽  
Highly Sensitive ◽  
Simultaneous Separation ◽  
Sensitive Determination ◽  
Highly Sensitive Detection ◽  
Determination Of Copper ◽  
Large Volume Sample Stacking

A CE-UV method has been developed for the simultaneous separation and sensitive determination of copper(i) and copper(ii) in cell specimens.

On-line preconcentration and determination of six sulfonylurea herbicides in cereals by MEKC with large-volume sample stacking and polarity switching

2013 ◽  
Vol 34 (9-10) ◽  
pp. 1304-1311 ◽  
Author(s):  
Ling-Xiao Yi ◽  
Guan-Hua Chen ◽  
Rou Fang ◽  
Li Zhang ◽  
Yu-Xiu Shao ◽  
...  
Keyword(s):  
Large Volume ◽  
Sulfonylurea Herbicides ◽  
Sample Stacking ◽  
Polarity Switching ◽  
On Line ◽  
Large Volume Sample Stacking
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