Proton chemical shifts in NMR spectroscopy. Part 7.1 C–C anisotropy and the methyl effect

1997 ◽  
pp. 31-40 ◽  
Author(s):  
Raymond J. Abraham ◽  
Mark A. Warne ◽  
Lee Griffiths
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Shifts ◽  
Methyl Effect ◽  
Proton Chemical Shifts

scholarly journals MgF3− and AlF4− transition state analogue complexes of yeast phosphoglycerate kinase

2017 ◽  
Vol 95 (2) ◽  
pp. 295-303
Author(s):  
Nicole E. McCormick ◽  
Stephanie M. Forget ◽  
Raymond T. Syvitski ◽  
David L. Jakeman
Keyword(s):  
Nmr Spectroscopy ◽  
Transition State ◽  
Chemical Shifts ◽  
Phosphoglycerate Kinase ◽  
Indirect Evidence ◽  
Metal Fluoride ◽  
Binding Affinities ◽  
Transition State Analogue ◽  
Transition State Analogues ◽  
Proton Chemical Shifts

The phospho-transfer mechanism of yeast phosphoglycerate kinase (PGK) has been probed through formation of trifluoromagnesate (MgF3−) and tetrafluoroaluminate (AlF4−) transition state analogue complexes and analyzed using 19F, 1H waterLOGSY and 1H chemical shift perturbation NMR spectroscopy. We observed the first 19F NMR spectroscopic evidence for the formation of metal fluoride transition state analogues of yeast PGK and also observed significant changes to proton chemical shifts of PGK in the presence, but not in the absence, of fluoride upon titration of ligands, providing indirect evidence of the formation of a closed ternary transition state. WaterLOGSY NMR spectroscopy experiments using an uncompetitive model were used in an attempt to measure ligand binding affinities within the transition state analogue complexes.

scholarly journals Resolving Nitrogen-15 and Proton Chemical Shifts for Mobile Segments of Elastin with Two-dimensional NMR Spectroscopy

2012 ◽  
Vol 287 (22) ◽  
pp. 18201-18209 ◽  
Author(s):  
Kosuke Ohgo ◽  
Walter P. Niemczura ◽  
Brian C. Seacat ◽  
Steven G. Wise ◽  
Anthony S. Weiss ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Shifts ◽  
Two Dimensional ◽  
Proton Chemical Shifts
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