scholarly journals Metabolomic characterisation of the effects of oncogenic PIK3CA transformation in a breast epithelial cell line

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Chung-Ho E. Lau ◽  
Gregory D. Tredwell ◽  
James K. Ellis ◽  
Eric W.-F. Lam ◽  
Hector C. Keun
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Breast Epithelial Cell Line ◽  
Breast Epithelial

scholarly journals The in vitro effects of 2-methoxyestradiol-bis-sulphamate on cell numbers, membrane integrity and cell morphology, and the possible induction of apoptosis and autophagy in a non-tumorigenic breast epithelial cell line

2010 ◽  
Vol 15 (4) ◽  
Author(s):  
Michelle Visagie ◽  
Annie Joubert
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Membrane Integrity ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Cell Numbers ◽  
Breast Epithelial Cell Line ◽  
Induction Of Apoptosis ◽  
Breast Epithelial

Abstract2-methoxyestradiol (2ME2) exerts estrogen receptor-independent anti-proliferative, anti-angiogenic and anti-tumor activity in vitro and in vivo. Due to its low bioavailability and rapid metabolic degradation, several analogues have been developed in recent years. 2-methoxyestradiol-bis-sulphamate (2-MeOE2bisMATE) is a bis-sulphamoylated derivative of 2ME2 with anti-proliferative activity. The aim of this study was to investigate cell signaling events induced by 2-MeOE2bisMATE in a non-tumorigenic cell line (MCF-12A) by analysing its influence on cell number, morphology and membrane integrity, and the possible induction of apoptosis and autophagy. Dose- and time-dependent studies revealed that 48 h exposure to 2-MeOE2bisMATE (0.4 μM) resulted in a decrease in cell numbers to 79%. A slight increase in the level of lactate dehydrogenase production was observed in the 2-MeOE2bisMATE-treated cells. Morphological studies revealed an increase in the number of cells in metaphase. Hallmarks of apoptosis were also found, namely nuclear fragmentation and apoptotic bodies. In addition, increased lysosomal staining was observed via fluorescent microscopy, suggesting the induction of another type of cell death, namely autophagy. Since 2-MeOE2bisMATE is regarded as a potential anti-cancer agent, it is also imperative to investigate the susceptibility of non-tumorigenic cells to its influence. The data generated from this study contributes to the understanding of the action that 2-MeOE2bisMATE exerts on the non-tumorigenic MCF-12A breast epithelial cell line.

Selection for EGFR gene amplification in a breast epithelial cell line with basal-like phenotype and hereditary background

2010 ◽  
Vol 47 (2) ◽  
pp. 139-148
Author(s):  
Saevar Ingthorsson ◽  
Thorhallur Halldorsson ◽  
Valgardur Sigurdsson ◽  
Agla JR Friðriksdottir ◽  
Sigridur K. Bodvarsdottir ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Gene Amplification ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Egfr Gene ◽  
Breast Epithelial Cell Line ◽  
Selection For ◽  
Breast Epithelial

scholarly journals Antisense expression for amphiregulin suppresses tumorigenicity of a transformed human breast epithelial cell line

Oncogene ◽  
1999 ◽  
Vol 18 (47) ◽  
pp. 6513-6520 ◽  
Author(s):  
Lin Ma ◽  
Christiane Gauvillé ◽  
Yolande Berthois ◽  
Guy Millot ◽  
Gibbes R Johnson ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Human Breast ◽  
Antisense Expression ◽  
Human Breast Epithelial Cell ◽  
Breast Epithelial Cell Line ◽  
Breast Epithelial

Morphological expression of cell transformation induced by c-Ha-ras oncogene in human breast epithelial cells

1991 ◽  
Vol 99 (2) ◽  
pp. 453-463
Author(s):  
J. Russo ◽  
L. Tait ◽  
I.H. Russo
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Morphological Changes ◽  
Cell Types ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Ras Oncogene ◽  
Human Breast ◽  
Breast Epithelial Cell Line ◽  
Breast Epithelial

The present work describes the morphological pattern of c-Ha-ras-transformed MCF-10A cells. This immortalized human breast epithelial cell line was transfected utilizing the calcium phosphate technique with pHo6 containing the neomycin-resistant gene alone, and identified as MCF-10Aneo, or with the normal Ha-ras proto-oncogene, MCF-10AneoN, or with the human p24 mutated Ha-ras oncogene, MCF-10AneoT cells. These three cell types were studied by scanning and transmission electron microscopy at passages 6 and 20 post-transfection. It was observed that transfection with the plasmid alone did not induce any morphological changes in MCF-10A cells. These two cell types exhibited those features that are characteristic of mammary epithelial cells in culture. Amplification of the normal c-Ha-ras oncogene by transfection induced significant morphological changes at the level of cell shape, from flat to cuboidal, and cytoplasmic changes suggesting a more metabolically active cell. These changes were made more prominent by transfection with the mutated ras oncogene, which induced stratification of a cuboidal epithelium and increase in cell size as well as a more pleomorphic nuclear and cytoplasmic appearance. Distinctive features induced by the mutated c-Ha-ras oncogene were the lengthening and thickening of cell surface microvilli, formation of blebs and emission of filopodial projections. It induced cytoplasmic changes consisting of formation of intracellular lumina, and increased the number of lysosomes, mitochondria and glycogen content, significantly decreasing the number of intermediate filaments. This is the first report that describes the morphological characteristics of a human breast epithelial cell line transformed in vitro.

Sign in / Sign up

Export Citation Format

Share Document