scholarly journals Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Tatiana C. A. Pinto ◽  
Natalia S. Costa ◽  
Luciana F. S. Castro ◽  
Rachel L. Ribeiro ◽  
Ana Caroline N. Botelho ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Alternative Approach ◽  
Capsular Typing ◽  
Tof Ms

scholarly journals Development of a novel MALDI-TOF MS-based bile solubility test for rapid discrimination of Streptococcus pneumoniae

2020 ◽  
Vol 310 (3) ◽  
pp. 151413 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Andreas Schlattmann ◽  
Markus Kostrzewa ◽  
Karsten Becker
Keyword(s):  
Streptococcus Pneumoniae ◽  
Solubility Test ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rapid Discrimination ◽  
Tof Ms

scholarly journals Accurate Differentiation of Streptococcus pneumoniae from other Species within the Streptococcus mitis Group by Peak Analysis Using MALDI-TOF MS

2017 ◽  
Vol 8 ◽  
Author(s):  
Mercedes Marín ◽  
Emilia Cercenado ◽  
Carlos Sánchez-Carrillo ◽  
Adrián Ruiz ◽  
Álvaro Gómez González ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Streptococcus Mitis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Utility of MALDI-TOF MS as a new tool for Streptococcus pneumoniae serotyping

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0212022 ◽  
Author(s):  
María Ercibengoa ◽  
Marta Alonso ◽  
Diego Vicente ◽  
Maria Morales ◽  
Ernesto Garcia ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals MALDI-TOF Mass Spectrometry-Based Optochin Susceptibility Testing for Differentiation of Streptococcus pneumoniae from other Streptococcus mitis Group Streptococci

2021 ◽  
Vol 9 (10) ◽  
pp. 2010
Author(s):  
Ilka D. Nix ◽  
Evgeny A. Idelevich ◽  
Andreas Schlattmann ◽  
Katrin Sparbier ◽  
Markus Kostrzewa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Streptococcus Pneumoniae ◽  
Incubation Time ◽  
Susceptibility Test ◽  
Test Accuracy ◽  
Streptococcus Mitis ◽  
Pathogenic Potential ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Discrimination of Streptococcus pneumoniae from other Streptococcus mitis group (SMG) species is still challenging but very important due to their different pathogenic potential. In this study, we aimed to develop a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based optochin susceptibility test with an objective read-out. Optimal test performance was established and evaluated by testing consecutively collected respiratory isolates. Optochin in different concentrations as a potential breakpoint concentration was added to a standardized inoculum. Droplets of 6 µL with optochin and, as growth control, without optochin were spotted onto a MALDI target. Targets were incubated in a humidity chamber, followed by medium removal and on-target protein extraction with formic acid before adding matrix with an internal standard. Spectra were acquired, and results were interpreted as S. pneumoniae in the case of optochin susceptibility (no growth), or as non-S. pneumoniae in the case of optochin non-susceptibility (growth). Highest test accuracy was achieved after 20 h incubation time (95.7%). Rapid testing after 12 h incubation time (optochin breakpoint 2 µg/mL; correct classification 100%, validity 62.5%) requires improvement by optimization of assay conditions. The feasibility of the MALDI-TOF MS-based optochin susceptibility test was demonstrated in this proof-of-principle study; however, confirmation and further improvements are warranted.

MALDI-TOF MS: an alternative approach for ribotyping Clostridioides difficile isolates in Brazil

Anaerobe ◽  
2021 ◽  
pp. 102351
Author(s):  
Carneiro Leandro Gouveia ◽  
Pinto Tatiana Castro Abreu ◽  
Moura Hercules ◽  
Barr John ◽  
Domingues Regina Maria Cavalcanti Pilotto ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Clostridioides Difficile ◽  
Alternative Approach ◽  
Tof Ms

scholarly journals Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae

2015 ◽  
Vol 34 (11) ◽  
pp. 2191-2198 ◽  
Author(s):  
S. Nakano ◽  
Y. Matsumura ◽  
Y. Ito ◽  
T. Fujisawa ◽  
B. Chang ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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