scholarly journals Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ying Chi ◽  
Yiyue Ge ◽  
Kangchen Zhao ◽  
Bingjie Zou ◽  
Bin Liu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Reverse Transcription ◽  
Influenza A ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Integration of reverse transcriptase loop-mediated isothermal amplification with an immunochromatographic strip on a centrifugal microdevice for influenza A virus identification

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 718-725 ◽  
Author(s):  
J. H. Jung ◽  
B. H. Park ◽  
S. J. Oh ◽  
G. Choi ◽  
T. S. Seo
Keyword(s):  
Reverse Transcriptase ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus Detection ◽  
Isothermal Amplification ◽  
Immunochromatographic Strip ◽  
Virus Identification ◽  
Loop Mediated Isothermal Amplification

In this paper, we demonstrated an integrated centrifugal microdevice which could perform reverse transcriptase loop-mediated isothermal amplification and immunochromatographic strip based amplicon analysis for rapid, sensitive, and multiplex influenza A virus detection.

scholarly journals Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Hongmei Bao ◽  
Yuhui Zhao ◽  
Yunhe Wang ◽  
Xiaolong Xu ◽  
Jianzhong Shi ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Reverse Transcription ◽  
Influenza A ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
H7n9 Virus ◽  
Loop Mediated Isothermal Amplification ◽  
H7n9 Influenza Virus ◽  
Virus Rna ◽  
H7n9 Influenza

A novel influenza A (H7N9) virus has emerged in China. To rapidly detect this virus from clinical samples, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7N9 virus. The minimum detection limit of the RT-LAMP assay was 0.01 PFU H7N9 virus, making this method 100-fold more sensitive to the detection of the H7N9 virus than conventional RT-PCR. The H7N9 virus RT-LAMP assays can efficiently detect different sources of H7N9 influenza virus RNA (from chickens, pigeons, the environment, and humans). No cross-reactive amplification with the RNA of other subtype influenza viruses or of other avian respiratory viruses was observed. The assays can effectively detect H7N9 influenza virus RNA in drinking water, soil, cloacal swab, and tracheal swab samples that were collected from live poultry markets, as well as human H7N9 virus, in less than 30 min. These results suggest that the H7N9 virus RT-LAMP assays were efficient, practical, and rapid diagnostic methods for the epidemiological surveillance and diagnosis of influenza A (H7N9) virus from different resource samples.

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