scholarly journals A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ryuichi Hirota ◽  
Kenji Abe ◽  
Zen-ichiro Katsuura ◽  
Reiji Noguchi ◽  
Shigeaki Moribe ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Growth And Survival ◽  
Bacterial Growth And Survival

scholarly journals The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival

2016 ◽  
Vol 198 (24) ◽  
pp. 3265-3277 ◽  
Author(s):  
Pauline Moulin ◽  
Kévin Patron ◽  
Camille Cano ◽  
Mohamed Amine Zorgani ◽  
Emilie Camiade ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Bacterial Growth ◽  
Streptococcus Agalactiae ◽  
Binding Proteins ◽  
Biological Fluids ◽  
Infection Process ◽  
Content Type ◽  
Growth And Survival ◽  
Vital Functions ◽  
Bacterial Growth And Survival

ABSTRACT The Lmb protein of Streptococcus agalactiae is described as an adhesin that binds laminin, a component of the human extracellular matrix. In this study, we revealed a new role for this protein in zinc uptake. We also identified two Lmb homologs, AdcA and AdcAII, redundant binding proteins that combine with the AdcCB translocon to form a zinc-ABC transporter. Expression of this transporter is controlled by the zinc concentration in the medium through the zinc-dependent regulator AdcR. Triple deletion of lmb , adcA , and adcAII , or that of the adcCB genes, impaired growth and cell separation in a zinc-restricted environment. Moreover, we found that this Adc zinc-ABC transporter promotes S. agalactiae growth and survival in some human biological fluids, suggesting that it contributes to the infection process. These results indicated that zinc has biologically vital functions in S. agalactiae and that, under the conditions tested, the Adc/Lmb transporter constitutes the main zinc acquisition system of the bacterium. IMPORTANCE A zinc transporter, composed of three redundant binding proteins (Lmb, AdcA, and AdcAII), was characterized in Streptococcus agalactiae . This system was shown to be essential for bacterial growth and morphology in zinc-restricted environments, including human biological fluids.

scholarly journals Dishwashing sponges and brushes: Consumer practices and bacterial growth and survival

2021 ◽  
Vol 337 ◽  
pp. 108928
Author(s):  
Trond Møretrø ◽  
Birgitte Moen ◽  
Valérie L. Almli ◽  
Paula Teixeira ◽  
Vânia B. Ferreira ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Growth And Survival ◽  
Bacterial Growth And Survival

scholarly journals Altered protein secretion of Chlamydia trachomatis in persistently infected human endocervical epithelial cells

Microbiology ◽  
2011 ◽  
Vol 157 (10) ◽  
pp. 2759-2771 ◽  
Author(s):  
Jin Wang ◽  
Kyla M. Frohlich ◽  
Lyndsey Buckner ◽  
Alison J. Quayle ◽  
Miao Luo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Reproductive Tract ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Target Cells ◽  
Growth And Survival ◽  
Persistently Infected ◽  
Virulence Proteins ◽  
Human Reproductive ◽  
Bacterial Growth And Survival

Chlamydia trachomatis is the most common bacterial infection of the human reproductive tract globally; however, the mechanisms underlying the adaptation of the organism to its natural target cells, human endocervical epithelial cells, are not clearly understood. To secure its intracellular niche, C. trachomatis must modulate the host cellular machinery by secreting virulence factors into the host cytosol to facilitate bacterial growth and survival. Here we used primary human endocervical epithelial cells and HeLa cells infected with C. trachomatis to examine the secretion of bacterial proteins during productive growth and persistent growth induced by ampicillin. Specifically, we observed a decrease in secretable chlamydial protease-like activity factor (CPAF) in the cytosol of host epithelial cells exposed to ampicillin with no evident reduction of CPAF product by C. trachomatis. In contrast, the expression of CopN and Tarp was downregulated, suggesting that C. trachomatis responds to ampicillin exposure by selectively altering the expression of secretable proteins. In addition, we observed a greater accumulation of outer-membrane vesicles from C. trachomatis in persistently infected cells. Taken together, these results suggest that the regulation of both gene expression and the secretion of chlamydial virulence proteins is involved in the adaptation of the bacteria to a persistent infection state in human genital epithelial cells.

scholarly journals In vitro inhibitory effects of commercial antiseptics and disinfectants on foodborne and environmental bacterial strains

2020 ◽  
Vol 3 ◽  
pp. 54
Author(s):  
Abiola Isawumi ◽  
Jacob K. Donkor ◽  
Lydia Mosi
Keyword(s):  
Antibacterial Agents ◽  
Hospital Environment ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Public Health Importance ◽  
Antibiotic Resistant ◽  
Growth And Survival ◽  
Bacterial Growth And Survival

Background: Antibacterial agents, including disinfectants and antiseptics are commonly used to reduce bacterial loads. As they have a broad-spectrum of activity against bacteria, function either as a bactericidal or bacteriostatic. While bacterial antimicrobial resistance is increasing, disinfectants and antiseptics are still relevant antibacterial agents.   Methods:  This study investigated the in vitro inhibitory effects of commonly used antiseptics and disinfectants. Using standard disc diffusion methods, selected common household antibacterial agents were tested on resistant Staphylococcus aureus isolated from hospital environment and foodborne Escherichia coli and Bacillus species. Results: The study showed that the selected antibacterial agents were effective against the antibiotic resistant bacteria with appreciable zone of inhibition relative to the standard controls used. Conclusions: Though bacteria are consistently developing resistance to available antibiotics, disinfectants still inhibit bacterial growth and survival with considerable public health importance.

scholarly journals Distinctiveness of genes contributing to growth of Pseudomonas syringae in diverse host plant species

2020 ◽  
Author(s):  
Tyler C. Helmann ◽  
Adam M. Deutschbauer ◽  
Steven E. Lindow
Keyword(s):  
Pseudomonas Syringae ◽  
Bacterial Colonization ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Growth And Survival ◽  
Polysaccharide Synthesis ◽  
Plant Hosts ◽  
Cofactor Metabolism ◽  
Plant Families ◽  
Bacterial Growth And Survival

AbstractA variety of traits are necessary for bacterial colonization of the interior of plant hosts, including well-studied virulence effectors as well as other phenotypes contributing to bacterial growth and survival within the apoplast. High-throughput methods such as transposon sequencing (TnSeq) are powerful tools to identify such genes in bacterial pathogens. However, there is little information as to the distinctiveness of traits required for bacterial colonization of different hosts. Here, we utilize randomly barcoded TnSeq (RB-TnSeq) to identify the genes that contribute to the ability of Pseudomonas syringae strain B728a to grow within common bean (Phaseolus vulgaris), lima bean (Phaseolus lunatus), and pepper (Capsicum annuum); species representing two different plant families. The magnitude of contribution of most genes to apoplastic fitness in each of the plant hosts was similar. However, 50 genes significantly differed in their fitness contributions to growth within these species. These genes encoded proteins in various functional categories including polysaccharide synthesis and transport, amino acid metabolism and transport, cofactor metabolism, and phytotoxin synthesis and transport. Six genes that encoded unannotated, hypothetical proteins also contributed differentially to growth in these hosts. The genetic repertoire of a relatively promiscuous pathogen such as P. syringae may thus be shaped, at least in part, by the conditional contribution of some fitness determinants.

scholarly journals In vitro inhibitory effects of commercial antiseptics and disinfectants on foodborne and environmental bacterial strains

2021 ◽  
Vol 3 ◽  
pp. 54
Author(s):  
Abiola Isawumi ◽  
Jacob K. Donkor ◽  
Lydia Mosi
Keyword(s):  
Antibacterial Agents ◽  
Hospital Environment ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Public Health Importance ◽  
Antibiotic Resistant ◽  
Growth And Survival ◽  
Bacterial Growth And Survival

Background: Antibacterial agents, including disinfectants and antiseptics are commonly used to reduce bacterial loads. As they have a broad-spectrum of activity against bacteria, function either as bactericidal or bacteriostatic agents. While bacterial antimicrobial resistance is increasing, disinfectants and antiseptics are still relevant antibacterial agents.   Methods:  This study investigated the in vitro inhibitory effects of commonly used antiseptics and disinfectants. Using standard disc diffusion methods, selected common household antibacterial agents were tested on resistant Staphylococcus aureus isolated from hospital environment and foodborne Escherichia coli and Bacillus species. Results: The study showed that the selected antibacterial agents were effective against the antibiotic resistant bacteria with appreciable zone of inhibition relative to the standard controls used. Conclusions: Though bacteria are consistently developing resistance to available antibiotics, disinfectants still inhibit bacterial growth and survival with considerable public health importance.

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