scholarly journals Erratum: Corrigendum: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yihong Lu ◽  
Cheng Fang ◽  
Qinhong Wang ◽  
Yuling Zhou ◽  
Guimin Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Codon Optimization ◽  
High Density ◽  
High Level Expression ◽  
Multiple Gene ◽  
Gene Insertion ◽  
High Level

scholarly journals High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yihong Lu ◽  
Cheng Fang ◽  
Qinhong Wang ◽  
Yuling Zhou ◽  
Guimin Zhang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Pichia Pastoris ◽  
Paper Industry ◽  
Xylanase Activity ◽  
Multiple Gene ◽  
E Coli ◽  
Gene Insertion ◽  
Alkaline Conditions ◽  
Pulp Properties ◽  
High Level

Abstract In paper industry, xylanases are used to increase the pulp properties in bleaching process as its eco-friendly nature. The xylanases activity is hindered by high temperature and alkaline conditions with high enzyme production cost in the paper industry. Here, XynHB, an alkaline stable xylanase from Bacillus pumilus HBP8 was mutated at N188A to XynHBN188A. Expressed mutant in E. coli showed 1.5-fold higher xylanase activity than XynHB at 60 °C. The mutant expressed in Pichia pastoris was glycosylated, remained stable for 30 min at 60 °C. XynHBN188A optimized based on codon usage bias for P. pastoris (xynHBN188As) showed an increase of 39.5% enzyme activity. The strain Y16 forming the largest hydrolysis halo in the xylan plate was used in shake flask experiments produced an enzyme activity of 6,403 U/ml. The Y16 strain had 9 copies of the recombinant xynHBN188As gene in the genome revealed by qPCR. The enzymatic activity increased to 48,241 U/ml in a 5 L fermentor. Supplement of 15 U/g xylanase enhanced the brightness of paper products by 2% in bleaching experiment, and thereby improved the tensile strength and burst factor by 13% and 6.5%, respectively. XynHBN188As has a great potential in paper industries.

High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization

2011 ◽  
Vol 34 (3) ◽  
pp. 507-514 ◽  
Author(s):  
Zhaowei Gao ◽  
Zhuofu Li ◽  
Yuhong Zhang ◽  
Huoqing Huang ◽  
Mu Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Glucose Oxidase ◽  
Codon Optimization ◽  
High Level Expression ◽  
Penicillium Notatum ◽  
Oxidase Gene ◽  
High Level ◽  
Glucose Oxidase Gene

scholarly journals High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris

2016 ◽  
Vol 193 ◽  
pp. 48-56 ◽  
Author(s):  
La Xiang ◽  
Qinhong Wang ◽  
Yuling Zhou ◽  
Lifeng Yin ◽  
Guimin Zhang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Codon Optimization ◽  
High Level Expression ◽  
High Level

High-level expression of cockroach allergen, Bla g 4, in Pichia pastoris☆☆☆★★★

1998 ◽  
Vol 101 (2) ◽  
pp. 274-280 ◽  
Author(s):  
Lisa D. Vailes ◽  
Michael T. Kinter ◽  
L.Karla Arruda ◽  
Martin D. Chapman
Keyword(s):  
Pichia Pastoris ◽  
High Level Expression ◽  
Cockroach Allergen ◽  
High Level
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