scholarly journals Quantum-dot-based suspension microarray for multiplex detection of lung cancer markers: preclinical validation and comparison with the Luminex xMAP® system

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Regina Bilan ◽  
Amagoia Ametzazurra ◽  
Kristina Brazhnik ◽  
Sergio Escorza ◽  
David Fernández ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Quantum Dot ◽  
Multiplex Detection ◽  
Cancer Markers

Ultrasensitive multiplex detection of structural rearrangements in ALK, RET, ROS1 and PD-L1 using a comprehensive next-generation sequencing assay.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. 3572-3572
Author(s):  
Kao Chin Ngeow ◽  
Yukti Choudhury ◽  
Kian-Hin Tan ◽  
Michelle Pek ◽  
Kim-Tien Ng ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Next Generation Sequencing ◽  
T Cell ◽  
Tumor Tissue ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Structural Rearrangements ◽  
Multiplex Detection ◽  
Nk T Cell ◽  
Generation Sequencing

3572 Background: Oncogenic structural rearrangements (SR) in ALK, RET and ROS1 are well-described in lung cancer, and confer sensitivity to targeted therapy. SR disrupting the 3’UTR of PD-L1 gene have been reported in multiple cancer types and can potentially predict response to checkpoint immunotherapy. An amplicon-based next-generation sequencing (NGS) platform technology (AmpliMARK), previously optimized for detection of single nucleotide variations (SNVs), microsatellite instability and viral DNA, was extended to the multiplex detection of SR in ALK, RET, ROS1 and PD-L1 in cell-free DNA (cfDNA) and tumor tissue DNA. Methods: A hybrid primer-extension and adapter-ligation based method allowing detection of SR in a fusion-partner agnostic manner was utilized for multiplex target capture of genomic regions of ALK, RET, ROS1 and PD-L1 SR. Analytical validation was performed using admixtures of fragmented genomic DNA from an ALK SR-positive cell line, commercial standards containing RET and ROS1 SR, and synthetic PD-L1 SR gene constructs. Clinical performance was assessed in cfDNA samples from lung cancer patients and tumor tissue DNA samples from natural killer(NK)/T-cell lymphoma patients. Results: Detection of SR could be achieved to an allele frequency detection limit of 0.5% with sensitivity of 89.5% and specificity of 100% in admixture samples mimicking cfDNA. In an unselected series of 374 lung cancer cases, actionable SR for ALK, RET and ROS1 were detected in cfDNA of 9 samples, for an overall detection rate of 2.4%, and 1.8% (3 out of 168) when restricted to treatment-naive lung cancer cases only. In 29 NK/T-cell lymphoma tumor tissue samples, 9 samples were positive for PD-L1 SR, which were orthogonally confirmed by whole-genome sequencing, targeted sequencing or Sanger sequencing for a concordance rate of 100% across all samples. For 1 NK/T-cell lymphoma tumor tissue sample where matched plasma was available, the same PD-L1 SR was also detected in cfDNA. Conclusions: We have demonstrated and validated a comprehensive amplicon-based NGS assay for ultrasensitive multiplex detection of structural rearrangements in ALK, RET, ROS1 and PD-L1 across both cfDNA and tumor tissue DNA in analytical and clinical contexts. Ongoing studies will further evaluate the performance and utility of this assay across a larger number of clinical samples for the detection of these SR as well as additional cancer-associated SR involving NTRK1/2/3, FGFR2/3 and TMPRSS2.

Magnetic beads-based electrochemiluminescence immunosensor for determination of cancer markers using quantum dot functionalized PtRu alloys as labels

The Analyst ◽  
2012 ◽  
Vol 137 (9) ◽  
pp. 2176 ◽  
Author(s):  
Yan Zhang ◽  
Shenguang Ge ◽  
Shaowei Wang ◽  
Mei Yan ◽  
Jinghua Yu ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Magnetic Beads ◽  
Cancer Markers

scholarly journals Multiplexed Analysis of Serum Breast and Ovarian Cancer Markers by Means of Suspension Bead–quantum Dot Microarrays

2015 ◽  
Vol 73 ◽  
pp. 235-240 ◽  
Author(s):  
Kristina Brazhnik ◽  
Zinaida Sokolova ◽  
Maria Baryshnikova ◽  
Regina Bilan ◽  
Igor Nabiev ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Quantum Dot ◽  
Breast And Ovarian Cancer ◽  
Cancer Markers ◽  
Multiplexed Analysis

Simultaneous immunoassay for the detection of two lung cancer markers using functionalized SERS nanoprobes

2011 ◽  
Vol 47 (46) ◽  
pp. 12515 ◽  
Author(s):  
Hyangah Chon ◽  
Sangyeop Lee ◽  
Soo-Young Yoon ◽  
Soo-Ik Chang ◽  
Dong Woo Lim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Markers ◽  
Simultaneous Immunoassay

scholarly journals Pretreatment Methods for Selections Lung Cancer Markers in Mouse Urine

2013 ◽  
Vol 62 (5) ◽  
pp. 437-442
Author(s):  
Yosuke HANAI ◽  
Yoshinobu BABA
Keyword(s):  
Lung Cancer ◽  
Cancer Markers ◽  
Mouse Urine
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