scholarly journals Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Payam Shahi ◽  
Samuel C. Kim ◽  
John R. Haliburton ◽  
Zev J. Gartner ◽  
Adam R. Abate
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Protein Profiling ◽  
Cell Protein

Integrated Proteome Analysis Device for Fast Single-Cell Protein Profiling

2018 ◽  
Vol 90 (23) ◽  
pp. 14003-14010 ◽  
Author(s):  
Xi Shao ◽  
Xuantang Wang ◽  
Sheng Guan ◽  
Haizhu Lin ◽  
Guoquan Yan ◽  
...  
Keyword(s):  
Single Cell ◽  
Proteome Analysis ◽  
Single Cell Protein ◽  
Protein Profiling ◽  
Cell Protein

scholarly journals Single-Cell Protein Profiling of Wastewater Enterobacterial Communities Predicts Disinfection Efficiency

2003 ◽  
Vol 69 (7) ◽  
pp. 4227-4235 ◽  
Author(s):  
Gomathinayagam Ponniah ◽  
Han Chen ◽  
Ronda Michielutti ◽  
Nancy Salonen ◽  
Paul Blum
Keyword(s):  
Single Cell ◽  
Municipal Wastewater ◽  
Heat Exposure ◽  
Treatment Plant ◽  
Single Cell Protein ◽  
Protein Profiling ◽  
Plant Performance ◽  
Cell Protein ◽  
Treatment Processes ◽  
Culture Independent

ABSTRACT The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater processing is unclear. The purpose of this study was to investigate this relationship. The influence of wastewater treatment processes on enterobacterial community physiology was examined at the single-cell level by using culture-independent methods. Intracellular concentrations of two conserved proteins, the growth-related protein Fis and the stationary-phase protein Dps, were analyzed by epifluoresence microscopy of uncultivated cells by using enterobacterial group-specific polyclonal fluorochrome-coupled antibodies. Enterobacterial single-cell community protein profiles were distinct for different types of biological treatment. The differences were not apparent when bulk methods of protein analysis were used. Trickling filter wastewater yielded Fis-enriched communities compared to the communities in submerged aeration basin wastewater. Community differences in Fis and Dps contents were used to predict disinfection efficiency. Disinfection of community samples by heat exposure combined with cultivation in selective media confirmed that enterobacterial communities exhibited significant differences in sensitivity to disinfection. These findings provide strategies that can be used to increase treatment plant performance, reduce the enterobacterial content in municipal wastewater, and minimize the release of disinfection by-products into receiving water.

Single-cell protein profiling

Science ◽  
2020 ◽  
Vol 367 (6477) ◽  
pp. 522.11-524
Author(s):  
Gemma Alderton
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Protein Profiling ◽  
Cell Protein

scholarly journals Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays

2018 ◽  
Vol 5 (9) ◽  
pp. 1800672 ◽  
Author(s):  
Peng Zhao ◽  
Sirsendu Bhowmick ◽  
Jianchao Yu ◽  
Jun Wang
Keyword(s):  
Single Cell ◽  
Large Scale ◽  
Single Cell Protein ◽  
Protein Profiling ◽  
Cell Protein

scholarly journals Single-cell protein profiling in microchambers with barcoded beads

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Lucas Armbrecht ◽  
Rafael Sebastian Müller ◽  
Jonas Nikoloff ◽  
Petra Stephanie Dittrich
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
Single Cells ◽  
Single Cell Protein ◽  
Protein Quantification ◽  
Protein Profiling ◽  
Cell Protein ◽  
Breast Cancer Cell Lines ◽  
Mammalian Cell Lines ◽  
Galectin 3

Abstract Single-cell profiling provides insights into cellular behaviour that macroscale cell cultures and bulk measurements cannot reveal. In the context of personalized cancer treatment, the profiling of individual tumour cells may lead to higher success rates for therapies by rapidly selecting the most efficacious drugs. Currently, genomic analysis at the single-cell level is available through highly sensitive sequencing approaches. However, the identification and quantification of intracellular or secreted proteins or metabolites remains challenging. Here, we introduce a microfluidic method that facilitates capture, automated data acquisition and the multiplexed quantification of proteins from individual cells. The microfluidic platform comprises 1026 chambers with a volume of 152 pL each, in which single cells and barcoded beads are co-immobilized. We demonstrated multiplexed single-cell protein quantification with three different mammalian cell lines, including two model breast cancer cell lines. We established on-chip immunoassays for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), galectin-3 (Gal-3) and galectin-3 binding protein (Gal-3bp) with detection limits as low as 7.0 × 104, 2.3 × 105 and 1.8 × 103 molecules per cell, respectively. The three investigated cell types had high cytosolic levels of GAPDH and could be clearly differentiated by their expression levels of Gal-3 and Gal-3bp, which are important factors that contribute to cancer metastasis. Because it employed commercially available barcoded beads for this study, our platform could be easily used for the single-cell protein profiling of several hundred different targets. Moreover, this versatile method is applicable to the analysis of bacteria, yeast and mammalian cells and nanometre-sized lipid vesicles.

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