scholarly journals Rat umbilical cord blood cells attenuate hypoxic–ischemic brain injury in neonatal rats

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Keiko Nakanishi ◽  
Yoshiaki Sato ◽  
Yuka Mizutani ◽  
Miharu Ito ◽  
Akihiro Hirakawa ◽  
...  
Keyword(s):  
Brain Injury ◽  
Cord Blood ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ischemic Brain Injury ◽  
Neonatal Rats ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Early administration of umbilical cord blood cells exacerbates ventilation-induced brain injury in preterm lambs

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S82
Author(s):  
Kyra Chan ◽  
Valerie Zahra ◽  
Vanesa Stojanovska ◽  
Paris Papagianis ◽  
Anqi Li ◽  
...  
Keyword(s):  
Brain Injury ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Early Administration ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Administration of Umbilical Cord Blood Cells Transiently Decreased Hypoxic-Ischemic Brain Injury in Neonatal Rats

2015 ◽  
Vol 37 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Tetsuo Hattori ◽  
Yoshiaki Sato ◽  
Taiki Kondo ◽  
Yuko Ichinohashi ◽  
Yuichiro Sugiyama ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Control Group ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Hypoxic Ischemic Brain Injury ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells ◽  
Number Of Cells

This study aimed to investigate whether the administration of mononuclear cells derived from human umbilical cord blood cells (UCBCs) could ameliorate hypoxic-ischemic brain injury in a neonatal rat model. The left carotid arteries of 7-day-old rats were ligated, and the rats were then exposed to 8% oxygen for 60 min. Mononuclear cells derived from UCBCs using the Ficoll-Hypaque technique were injected intraperitoneally 6 h after the insult (1.0 × 107 cells). Twenty-four hours after the insult, the number of cells positive for the oxidative stress markers 4-hydroxy-2-nonenal and nitrotyrosine, in the dentate gyrus of the hippocampus in the UCBC-treated group, decreased by 36 and 42%, respectively, compared with those in the control group. In addition, the number of cells positive for the apoptosis markers active caspase-3 and apoptosis-inducing factor decreased by 53 and 58%, respectively. The number of activated microglia (ED1-positive cells) was 51% lower in the UCBC group compared with the control group. In a gait analysis performed 2 weeks after the insult, there were no significant differences among the sham-operated, control and UCBC groups. An active avoidance test using a shuttle box the following week also revealed no significant differences among the groups. Neither the volumes of the hippocampi, corpus callosum and cortices nor the numbers of neurons in the hippocampus were different between the UCBC and control groups. In summary, a single intraperitoneal injection of UCBC-derived mononuclear cells 6 h after an ischemic insult was associated with a transient reduction in numbers of apoptosis and oxidative stress marker-positive cells, but it did not induce long-term morphological or functional protection. Repeated administration or a combination treatment may be required to achieve sustained protection.

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