scholarly journals Polymerase cross-linking spiral reaction (PCLSR) for detection of African swine fever virus (ASFV) in pigs and wild boars

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Grzegorz Woźniakowski ◽  
Magdalena Frączyk ◽  
Andrzej Kowalczyk ◽  
Małgorzata Pomorska-Mól ◽  
Krzysztof Niemczuk ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Cross Linking ◽  
Wild Boars

scholarly journals Rynek mięsa wieprzowego w Polsce w obliczu afrykańskiego pomoru świń (ASF)

2018 ◽  
Vol 18(33) (3) ◽  
pp. 306-314
Author(s):  
Mirosława Tereszczuk
Keyword(s):  
Negative Impact ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
First Year ◽  
Upward Trend ◽  
Wild Boars ◽  
Central Poland ◽  
The Impact ◽  
Descriptive Method

The aim of the study is to assess the impact of African swine fever virus (ASF) on the development and functioning of the pork market in Poland. This virus appeared in Poland in February 2014 in wild boars, and then in July 2014 in pigs. The study covers the years 2014-2018, the period of ASF virus in Poland. By the end of July 2018, there were 182 cases of ASF in pigs and over 2.6 thousand in wild boars in four provinces of eastern and central Poland, and the largest cluster of this disease is located in the Lublin province. The appearance of ASF virus has had a negative impact on the pork market in Poland, resulting in restrictions on the export of this meat, costs of eradication of outbreaks and biosecurity. Despite these difficulties, the production of pork at this time was characterized by an upward trend, while exports only in the first year of the ASF decreased, but in subsequent years, due to the diversification of markets, there was an increase in exports. The descriptive method and simple statistical analyzes were used for the study.

scholarly journals African swine fever virus infection in Classical swine fever subclinically infected wild boars

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Oscar Cabezón ◽  
Sara Muñoz-González ◽  
Andreu Colom-Cadena ◽  
Marta Pérez-Simó ◽  
Rosa Rosell ◽  
...  
Keyword(s):  
Virus Infection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Wild Boars

scholarly journals High Virulence of African Swine Fever Virus Caucasus Isolate in European Wild Boars of All Ages

2012 ◽  
Vol 18 (4) ◽  
Author(s):  
Sandra Blome ◽  
Claudia Gabriel ◽  
Klaas Dietze ◽  
Angele Breithaupt ◽  
Martin Beer
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Wild Boars ◽  
High Virulence

scholarly journals Swift and Reliable “Easy Lab” Methods for the Sensitive Molecular Detection of African Swine Fever Virus

2021 ◽  
Vol 22 (5) ◽  
pp. 2307
Author(s):  
Ahmed Elnagar ◽  
Jutta Pikalo ◽  
Martin Beer ◽  
Sandra Blome ◽  
Bernd Hoffmann
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Molecular Diagnostic ◽  
Economic Losses ◽  
Hemorrhagic Disease ◽  
Tissue Samples ◽  
Wild Boars ◽  
Field Samples ◽  
Edta Blood

African swine fever (ASF) is a contagious viral hemorrhagic disease of domestic pigs and wild boars. The disease is notifiable to the World Organisation for Animal Health (OIE) and is responsible for high mortality and serious economic losses. PCR and real-time PCR (qPCR) are the OIE-recommended standard methods for the direct detection of African swine fever virus (ASFV) DNA. The aim of our work was the simplification and standardization of the molecular diagnostic workflow in the lab. For validation of this “easy lab” workflow, different sample materials from animal trials were collected and analyzed (EDTA blood, serum, oral swabs, chewing ropes, and tissue samples) to identify the optimal sample material for diagnostics in live animals. Based on our data, the EDTA blood samples or bloody tissue samples represent the best specimens for ASFV detection in the early and late phases of infection. The application of prefilled ready-to-use reagents for nucleic acid extraction or the use of a Tissue Lysis Reagent (TLR) delivers simple and reliable alternatives for the release of the ASFV nucleic acids. For the qPCR detection of ASFV, different published and commercial kits were compared. Here, a lyophilized commercial kit shows the best results mainly based on the increased template input. The good results of the “easy lab” strategy could be confirmed by the ASFV detection in field samples from wild boars collected from the 2020 ASFV outbreak in Germany. Appropriate internal control systems for extraction and PCR are key features of the “easy lab” concept and reduce the risk of false-negative and false-positive results. In addition, the use of easy-to-handle machines and software reduces training efforts and the misinterpretation of results. The PCR diagnostics based on the “easy lab” strategy can realize a high sensitivity and specificity comparable to the standard PCR methods and should be especially usable for labs with limited experiences and resources.

scholarly journals Characteristics of African Swine Fever Virus Isolated from Domestic Pigs and Wild Boars in the Russian Federation and South Ossetia

2020 ◽  
Vol 70 (1) ◽  
pp. 58-70
Author(s):  
Mikhail Vlasov ◽  
Almaz Imatdinov ◽  
Ilya Titov ◽  
Nikola Vasković ◽  
Valentina Lyska ◽  
...  
Keyword(s):  
Russian Federation ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Genetic Characteristics ◽  
South Ossetia ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Irkutsk Region ◽  
The Russian Federation

AbstractThe article presents the results of a comparative study of the biological, molecular and genetic characteristics of African swine fever virus (ASFV) isolates, obtained from domestic pigs and wild boars in the Russian Federation and South Ossetia from 2016 to 2018. The studied isolates caused the death of pigs manifesting, as a rule, signs of an acute or subacute form of the disease when using various methods of infection including intramuscular, direct contact, intranasal and oral routes. The virus was hemadsorbing, belonging to serotype 8 and genotype II, and accumulated in the blood with a titer of 6.5 to 7.5 lg HAU50/cm3. The ASFV isolates circulating in the central region of Russia were found to have an insertion of 10 base pairs in the intergenic region I73R/I329L. However, the ASFV isolated in the Irkutsk region and South Ossetia, as well as Georgia 2007/1 (FR682468.1), lacked this insertion.

scholarly journals Wild boar behaviour and the epidemiological significance of this animal species as the main reservoir of the African swine fever virus

2021 ◽  
Vol 77 (02) ◽  
pp. 6501-2021
Author(s):  
KAZIMIERZ TARASIUK ◽  
ZYGMUNT GIŻEJEWSKI
Keyword(s):  
Wild Boar ◽  
Animal Species ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Annual Growth Rate ◽  
Wild Boar Population ◽  
Wild Boars ◽  
In The Wild ◽  
To Come

Recent years (since 2013) have witnessed an increasing interest in wild boars in Europe because this animal species has become the most important reservoir of the African swine fever virus (ASFV). In all countries affected by ASF since 2013, the virus originated in wild boar populations, and the disease was directly or indirectly transmitted from wild boars to domestic pigs. In Poland, the first known case of ASF was detected in February 2014, and nearly 10 300 cases were reported by the end of April 2020. A correlation was found between the density of wild boar populations and the number of ASF cases. The global population of wild boars has increased considerably, and their natural range has expanded, particularly northward and westward. The mean annual growth rate in the wild boar population in Europe is around 100%. Milder winters and agricultural transformations that involve growing a single crop (in particular, maize) over large areas are the key factors contributing to the increase in the number of wild boars. In the opinion of most experts, recreational hunting is not sufficient to control the expansion of wild boar populations in Europe. Undoubtedly, wild boar populations will continue to be the main reservoir of the ASFV for many years to come, and wild boars will remain the major risk factor for ASF spread in commercial pig herds. Therefore, a new long-term approach to wild boar population management is needed

scholarly journals Characterization of African Swine Fever Virus Caucasus Isolate in European Wild Boars

2011 ◽  
Vol 17 (12) ◽  
pp. 2342-2345 ◽  
Author(s):  
Claudia Gabriel ◽  
Sandra Blome ◽  
Alexander Malogolovkin ◽  
Stanislav Parilov ◽  
Denis Kolbasov ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Wild Boars

Genetic markers of the African swine fever virus

2020 ◽  
Vol 23 (04) ◽  
pp. 21-26
Author(s):  
A.K. Sibgatullova ◽  
◽  
M.E. Vlasov ◽  
I.A. Titov ◽  
◽  
...  
Keyword(s):  
Genetic Markers ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus
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