scholarly journals Identification of a novel type III secretion-associated outer membrane-bound protein from Xanthomonas campestris pv. campestris

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lei Li ◽  
Rui-Fang Li ◽  
Zhen-Hua Ming ◽  
Guang-Tao Lu ◽  
Ji-Liang Tang
Keyword(s):  
Outer Membrane ◽  
Type Iii Secretion ◽  
Xanthomonas Campestris ◽  
Type Iii ◽  
Membrane Bound ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Characterization, Phylogeny, and Genome Analyses of Nonpathogenic Xanthomonas campestris Strains Isolated from Brassica Seeds

2020 ◽  
Vol 110 (5) ◽  
pp. 981-988 ◽  
Author(s):  
Yung-An Lee ◽  
Pei-Yu Yang ◽  
Shau-Chang Huang
Keyword(s):  
Type Iii Secretion ◽  
Xanthomonas Campestris ◽  
Pathogenicity Island ◽  
Whole Genome Sequence ◽  
Effector Protein ◽  
Type Iii Effector ◽  
Type Iii ◽  
Effector Genes ◽  
Genome Analyses ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonads were detected by using the Xan-D(CCF) medium from the brassica seeds, and their pathogenicity was determined by plant inoculation tests. It was found that some seed lots were infested with Xanthomonas campestris pv. campestris, some with X. campestris pv. raphani, and some with nonpathogenic xanthomonads. The nonpathogenic xanthomonad strains were identified as X. campestris, and the multilocus sequence analysis showed that the nonpathogenic X. campestris strains were grouped together with pathogenic X. campestris, but not with nonpathogenic strains of X. arboricola. In addition, all isolated X. campestris pv. campestris and X. campestris pv. raphani strains were positive in the hrpF-PCR, but the nonpathogenic strains were negative. It was further found that nonpathogenic X. campestris strain nE1 does not contain the entire pathogenicity island (hrp gene cluster; type III secretion system) and all type III effector protein genes based on the whole genome sequence analyses. The nonpathogenic X. campestris strain nE1 could acquire the entire pathogenicity island from the endemic X. campestris pv. campestris and X. campestris pv. raphani strains by conjugation, but type III effector genes were not cotransferred. The studies showed that the nonpathogenic X. campestris strains indeed exist on the brassica seeds, but it could be differentiated by the PCR assays on the hrp and type III effector genes. Nevertheless, the nonpathogenic X. campestris strains cannot be ignored because they may be potential gene resources to increase genetic diversity in the endemic pathogenic X. campestris pv. campestris and X. campestris pv. raphani strains.

The type III secretion effector XopXccN of Xanthomonas campestris pv. campestris is required for full virulence

2008 ◽  
Vol 159 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Bo-Le Jiang ◽  
Yong-Qiang He ◽  
Wei-Jian Cen ◽  
Hong-Yu Wei ◽  
Guo-Feng Jiang ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Xanthomonas Campestris ◽  
Type Iii ◽  
Type Iii Secretion Effector ◽  
Xanthomonas Campestris Pv Campestris

scholarly journals Two Xanthomonas Extracellular Polygalacturonases, PghAxc and PghBxc, Are Regulated by Type III Secretion Regulators HrpX and HrpG and Are Required for Virulence

2008 ◽  
Vol 21 (5) ◽  
pp. 555-563 ◽  
Author(s):  
Lifeng Wang ◽  
Wei Rong ◽  
Chaozu He
Keyword(s):  
Type Iii Secretion ◽  
Xanthomonas Campestris ◽  
Type Ii Secretion ◽  
Dependent Manner ◽  
In Planta ◽  
Cell Wall Degrading Enzymes ◽  
Type Iii ◽  
Type Ii Secretion System ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonas campestris pv. campestris, the causal agent of black rot disease, produces a suite of extracellular cell-wall degrading enzymes (CWDE) that are involved in bacterial virulence. Polygalacturonase (PG) is an important CWDE and functions to degrade the pectic layers of plant cell walls. Although previous studies have documented the virulence functions of PG in Erwinia and Ralstonia species, the regulation of PG genes still needs to be elucidated. In this study, we identified two novel PG genes (pghAxc and pghBxc) encoding functional PG from X. campestris pv. campestris 8004. The expressions of these two PG genes are regulated by the type III secretion regulators HrpX and HrpG and the global regulator Clp. These PG genes could be efficiently induced in planta and were required for the full virulence of X. campestris pv. campestris to Arabidopsis. In addition, these PG were confirmed to be secreted via the type II secretion system in an Xps-dependent manner.

scholarly journals The Contribution of the Predicted Sorting Platform Component HrcQ to Type III Secretion in Xanthomonas campestris pv. vesicatoria Depends on an Internal Translation Start Site

2021 ◽  
Vol 12 ◽  
Author(s):  
Christian Otten ◽  
Tanja Seifert ◽  
Jens Hausner ◽  
Daniela Büttner
Keyword(s):  
Type Iii Secretion ◽  
Structural Component ◽  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Start Site ◽  
Translation Start Site ◽  
Terminal Protein ◽  
Type Iii ◽  
Translation Start ◽  
Membrane Spanning

Pathogenicity of the Gram-negative bacterium Xanthomonas campestris pv. vesicatoria depends on a type III secretion (T3S) system which translocates effector proteins into plant cells. T3S systems are conserved in plant- and animal-pathogenic bacteria and consist of at least nine structural core components, which are designated Sct (secretion and cellular translocation) in animal-pathogenic bacteria. Sct proteins are involved in the assembly of the membrane-spanning secretion apparatus which is associated with an extracellular needle structure and a cytoplasmic sorting platform. Components of the sorting platform include the ATPase SctN, its regulator SctL, and pod-like structures at the periphery of the sorting platform consisting of SctQ proteins. Members of the SctQ family form a complex with the C-terminal protein domain, SctQC, which is translated as separate protein and likely acts either as a structural component of the sorting platform or as a chaperone for SctQ. The sorting platform has been intensively studied in animal-pathogenic bacteria but has not yet been visualized in plant pathogens. We previously showed that the SctQ homolog HrcQ from X. campestris pv. vesicatoria assembles into complexes which associate with the T3S system and interact with components of the ATPase complex. Here, we report the presence of an internal alternative translation start site in hrcQ leading to the separate synthesis of the C-terminal protein region (HrcQC). The analysis of genomic hrcQ mutants showed that HrcQC is essential for pathogenicity and T3S. Increased expression levels of hrcQ or the T3S genes, however, compensated the lack of HrcQC. Interaction studies and protein analyses suggest that HrcQC forms a complex with HrcQ and promotes HrcQ stability. Furthermore, HrcQC colocalizes with HrcQ as was shown by fluorescence microscopy, suggesting that it is part of the predicted cytoplasmic sorting platform. In agreement with this finding, HrcQC interacts with the inner membrane ring protein HrcD and the SctK-like linker protein HrpB4 which contributes to the docking of the HrcQ complex to the membrane-spanning T3S apparatus. Taken together, our data suggest that HrcQC acts as a chaperone for HrcQ and as a structural component of the predicted sorting platform.

scholarly journals Chemical Structure, Biological Roles, Biosynthesis and Regulation of the Yellow Xanthomonadin Pigments in the Phytopathogenic Genus Xanthomonas

2020 ◽  
Vol 33 (5) ◽  
pp. 705-714 ◽  
Author(s):  
Ya-Wen He ◽  
Xue-Qiang Cao ◽  
Alan R. Poplawsky
Keyword(s):  
Chemical Structure ◽  
Xanthomonas Campestris ◽  
Bacterial Species ◽  
Regulatory Mechanisms ◽  
Chemical Structures ◽  
Considerable Research ◽  
Membrane Bound ◽  
Yellow Pigments ◽  
Epiphytic Survival ◽  
Xanthomonas Campestris Pv Campestris

Xanthomonadins are membrane-bound yellow pigments that are typically produced by phytopathogenic bacterial Xanthomonas spp., Xylella fastidiosa, and Pseudoxanthomonas spp. They are also produced by a diversity of environmental bacterial species. Considerable research has revealed that they are a unique group of halogenated, aryl-polyene, water-insoluble pigments. Xanthomonadins have been shown to play important roles in epiphytic survival and host-pathogen interactions in the phytopathogen Xanthomonas campestris pv. campestris, which is the causal agent of black rot in crucifers. Here, we review recent advances in the understanding of xanthomonadin chemical structures, physiological roles, biosynthetic pathways, regulatory mechanisms, and crosstalk with other signaling pathways. The aim of the present review is to provide clues for further in-depth research on xanthomonadins from Xanthomonas and other related bacterial species.

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