scholarly journals Comparative proteomic analysis of horseweed (Conyza canadensis) biotypes identifies candidate proteins for glyphosate resistance

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Fidel González-Torralva ◽  
Adrian P. Brown ◽  
Stephen Chivasa
Keyword(s):  
Proteomic Analysis ◽  
Glyphosate Resistance ◽  
Conyza Canadensis ◽  
Comparative Proteomic Analysis

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

scholarly journals Environmental cues affecting horseweed (Conyza canadensis) growth type and their sensitivity to glyphosate

Weed Science ◽  
2021 ◽  
pp. 1-35
Author(s):  
John A. Schramski ◽  
Christy L. Sprague ◽  
Eric L. Patterson
Keyword(s):  
Soil Moisture ◽  
Glyphosate Resistance ◽  
Single Parent ◽  
Environmental Cues ◽  
Conyza Canadensis ◽  
Growth Type ◽  
Susceptible Population ◽  
Water Imbibition ◽  
Growth Types ◽  
Winter Annual

Abstract Horseweed [Conyza canadensis (L.) Cronquist] is a facultative winter annual weed that can emerge from March to November in Michigan. Fall emerging C. canadensis overwinters as a rosette, while spring emerging C. canadensis skips the rosette stage and immediately grows upright upon emergence. In Michigan, primary emergence recently shifted from fall to spring/summer and therefore from a rosette to an upright growth type. Growth chamber experiments were conducted to determine 1) whether both C. canadensis growth types could originate from a single parent and 2) if common environmental cues can influence growth type. Variations in temperature, photoperiod, competition, shading, and soil moisture only resulted in the rosette growth type in four C. canadensis populations originating from seed collected from a single parent of the upright growth type. However, a vernalization period of four weeks following water imbibition, but prior to germination, resulted in the upright growth type. Dose-response experiments were conducted to determine whether glyphosate sensitivity differed between C. canadensis growth types generated from a single parent of the upright growth type. Upright type C. canadensis from known glyphosate-resistant populations ISB-18 and MSU-18 were four and three-fold less sensitive to glyphosate than their rosette siblings, respectively. Interestingly, differences in glyphosate sensitivity was not observed between growth types from the susceptible population. These results suggest that while C. canadensis populations shift from winter to summer annual lifecycles, concurrent increases in glyphosate resistance could occur.

scholarly journals Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0227226 ◽  
Author(s):  
Shun Tamaki ◽  
Kohei Nishino ◽  
Takahisa Ogawa ◽  
Takanori Maruta ◽  
Yoshihiro Sawa ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Hypoxic Conditions

Comparative proteomic analysis of whole saliva from chronic periodontitis patients

2010 ◽  
Vol 73 (7) ◽  
pp. 1334-1341 ◽  
Author(s):  
Lorena Da Rós Gonçalves ◽  
Márcia Regina Soares ◽  
Fábio C.S. Nogueira ◽  
Carlos Garcia ◽  
Danielle Resende Camisasca ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Whole Saliva

Comparative Proteomic Analysis of Indioside D-Triggered Cell Death in HeLa Cells

2008 ◽  
Vol 7 (5) ◽  
pp. 2050-2058 ◽  
Author(s):  
Chi Chun Wong ◽  
Ying Wang ◽  
Ka-Wing Cheng ◽  
Jen-Fu Chiu ◽  
Qing-Yu He ◽  
...  
Keyword(s):  
Cell Death ◽  
Hela Cells ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis
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