scholarly journals No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, Apis cerana cerana (Hymenoptera, Apidae)

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hui-Ru Jia ◽  
Ping-Li Dai ◽  
Li-Li Geng ◽  
Cameron J. Jack ◽  
Yun-He Li ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Honey Bees ◽  
Apis Cerana ◽  
Apis Cerana Cerana

Bt Cry1Ie Toxin Does Not Impact the Survival and Pollen Consumption of Chinese Honey Bees,Apis cerana cerana(Hymenoptera, Apidae)

2016 ◽  
Vol 109 (6) ◽  
pp. 2259-2263 ◽  
Author(s):  
Ping-Li Dai ◽  
Hui-Ru Jia ◽  
Cameron J. Jack ◽  
Li-Li Geng ◽  
Feng Liu ◽  
...  
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
Pollen Consumption

Molecular Characterization and Oxidative Stress Response of a Cytochrome P450 Gene (CYP4G11) from Apis cerana cerana

2013 ◽  
Vol 68 (11-12) ◽  
pp. 509-521 ◽  
Author(s):  
Weina Shi ◽  
Jing Sun ◽  
Baohua Xu ◽  
Han Li
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Honey Bees ◽  
Uv Light ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
High Sequence Identity ◽  
P450 Gene

Cytochrome P450 proteins, widely distributed multifunctional enzymes, are mainly involved in biosynthetic and degradative pathways of endogenous compounds and the detoxification of xenobiotics in insects. Moreover, these enzymes exhibit peroxidase-like activity, therefore they may be involved in protecting organisms against the toxicity of reactive oxygen species (ROS). In the present study, we cloned a CYP4G11 gene - AccCYP4G11 - from the Chinese honey-bee (Apis cerana cerana). The open reading frame of the cDNA was 1656 bp long and encoded a 551 amino acids polypeptide, which shared high sequence identity with homologous cytochrome P450 proteins. In the genomic DNA sequence, a 5'-flanking region consisting of 1168 bp was obtained, and some putative transcription factor binding sites were predicted. Quantitative polymerase chain reaction (Q-PCR) revealed that the level of AccCYP4G11 was higher in the epidermis than in other tissues, and Acc- CYP4G11 was expressed in all stages with the highest level in two-week-old adult worker honey-bees. More over, the expression patterns under oxidative stress indicated that Acc- CYP4G11 transcription was significantly influenced by external factors, such as temperature challenges, ultraviolet (UV) light, and insecticide treatment. AccCYP4G11 was regulated differentially in response to oxidative stress and may be involved in protecting honey-bees from oxidative injury.

scholarly journals The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

2013 ◽  
Vol 12 (3) ◽  
pp. 474-482 ◽  
Author(s):  
Wei-yu JIANG ◽  
Li-li GENG ◽  
Ping-li DAI ◽  
Zhi-hong LANG ◽  
Chang-long SHU ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Apis Cerana ◽  
Transgenic Maize ◽  
Apis Cerana Cerana ◽  
Maize Pollen

Contact Chemosensory Genes Identified in Leg Transcriptome of Apis cerana cerana (Hymenoptera: Apidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2015-2029 ◽  
Author(s):  
Yali Du ◽  
Kai Xu ◽  
Weihua Ma ◽  
Wenting Su ◽  
Miaomiao Tai ◽  
...  
Keyword(s):  
Honey Bees ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Apis Cerana ◽  
In Silico Analysis ◽  
Apis Cerana Cerana ◽  
Specific Expression ◽  
Genes Encoding ◽  
Honey Bee Workers ◽  
Selection Of

Abstract Correct gustatory recognition and selection of foods both within and outside the hive by honey bee workers are fundamental to the maintenance of colonies. The tarsal chemosensilla located on the legs of workers are sensitive to nonvolatile compounds and proposed to be involved in gustatory detection. However, little is known about the molecular mechanisms underlying the gustatory recognition of foods in honey bees. In the present study, RNA-seq was performed with RNA samples extracted from the legs of 1-, 10-, and 20-d-old workers of Apis cerana cerana Fabricius, a dominant indigenous crop pollinator with a keen perception ability for phytochemicals. A total of 124 candidate chemosensory proteins (CSPs), including 15 odorant-binding proteins (OBPs), 5 CSPs, 7 gustatory receptors (GRs), 2 sensory neuron membrane proteins (SNMPs), and 95 odorant receptors (ORs), were identified from the assembled leg transcriptome. In silico analysis of expression showed that 36 of them were differentially expressed among the three different ages of A. c. cerana workers. Overall, the genes encoding OBPs and CSPs had great but extremely variable FPKM values and thus were highly expressed in the legs of workers, whereas the genes encoding ORs, GRs, and SNMPs (except SNMP2) were expressed at low levels. Tissue-specific expression patterns indicated that two upregulated genes, AcerOBP15 and AcerCSP3, were predominately expressed in the legs of 20-d-old foragers, suggesting they may play an essential role in gustatory recognition and selection of plant nectars and pollens. This study lays a foundation for further research on the feeding preferences of honey bees.

scholarly journals Antiviral Activities of a Medicinal Plant Extract Against Sacbrood Virus in Honeybees

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Liping Sun ◽  
Xueqi Zhang ◽  
Shufa Xu ◽  
Chunsheng Hou ◽  
Jin Xu ◽  
...  
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Larval Growth ◽  
Antiviral Agent ◽  
Antiviral Effect ◽  
Absolute Quantification ◽  
Chinese Medicinal Herb ◽  
Sacbrood Virus ◽  
Antiviral Activities ◽  
Potential Strategy

Abstract Background Sacbrood is an infectious disease of the honey bee caused by Scbrood virus (SBV) which belongs to the family Iflaviridae and is especially lethal for Asian honeybee Apis cerana. Chinese Sacbrood virus (CSBV) is a geographic strain of SBV. Currently, there is a lack of an effective antiviral agent for controlling CSBV infection in honey bees. Methods Here, we explored the antiviral effect of a Chinese medicinal herb Radix isatidis on CSBV infection in A. cerana by inoculating the 3rd instar larvae with purified CSBV and treating the infected bee larvae with R. isatidis extract at the same time. The growth, development, and survival of larvae between the control and treatment groups were compared. The CSBV copy number at the 4th instar, 5th instar, and 6th instar larvae was measured by the absolute quantification PCR method. Results Bioassays revealed that R. isatidis extract significantly inhibited the replication of CSBV, mitigated the impacts of CSBV on larval growth and development, reduced the mortality of CSBV-infected A. cerana larvae, and modulated the expression of immune transcripts in infected bees. Conclusion Although the mechanism underlying the inhibition of CSBV replication by the medicine plant will require further investigation, this study demonstrated the antiviral activity of R. isatidis extract and provides a potential strategy for controlling SBV infection in honey bees.

Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana

2021 ◽  
Author(s):  
Guangdong Zhao ◽  
Wenchun Zhao ◽  
Xuepei Cui ◽  
Baohua Xu ◽  
Qingxin Liu ◽  
...  
Keyword(s):  
Apis Cerana ◽  
Apis Cerana Cerana
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