scholarly journals High-resolution traction force microscopy on small focal adhesions - improved accuracy through optimal marker distribution and optical flow tracking

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Claude N. Holenstein ◽  
Unai Silvan ◽  
Jess G. Snedeker
Keyword(s):  
High Resolution ◽  
Optical Flow ◽  
Focal Adhesions ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Marker Distribution ◽  
Improved Accuracy

scholarly journals High-Resolution Traction Force Microscopy

2014 ◽  
pp. 367-394 ◽  
Author(s):  
Sergey V. Plotnikov ◽  
Benedikt Sabass ◽  
Ulrich S. Schwarz ◽  
Clare M. Waterman
Keyword(s):  
High Resolution ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

scholarly journals High Resolution Traction Force Microscopy Based on Experimental and Computational Advances

2008 ◽  
Vol 94 (1) ◽  
pp. 207-220 ◽  
Author(s):  
Benedikt Sabass ◽  
Margaret L. Gardel ◽  
Clare M. Waterman ◽  
Ulrich S. Schwarz
Keyword(s):  
High Resolution ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

scholarly journals High Resolution, Large Deformation 3D Traction Force Microscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 493a ◽  
Author(s):  
Jennet Toyjanova ◽  
Eyal Bar-Kochba ◽  
Cristina Lopez-Fagundo ◽  
Jonathan Reichner ◽  
Diane Hoffman-Kim ◽  
...  
Keyword(s):  
High Resolution ◽  
Large Deformation ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

scholarly journals Optimization of traction force microscopy for micron-sized focal adhesions

2010 ◽  
Vol 22 (19) ◽  
pp. 194104 ◽  
Author(s):  
Jonathan Stricker ◽  
Benedikt Sabass ◽  
Ulrich S Schwarz ◽  
Margaret L Gardel
Keyword(s):  
Focal Adhesions ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

scholarly journals Multidimensional traction force microscopy reveals out-of-plane rotational moments about focal adhesions

2012 ◽  
Vol 110 (3) ◽  
pp. 881-886 ◽  
Author(s):  
W. R. Legant ◽  
C. K. Choi ◽  
J. S. Miller ◽  
L. Shao ◽  
L. Gao ◽  
...  
Keyword(s):  
Focal Adhesions ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Out Of Plane

scholarly journals A Focal Adhesion Filament Cross-correlation Kit for fast, automated segmentation and correlation of focal adhesions and actin stress fibers in cells

2021 ◽  
Author(s):  
Lara Hauke ◽  
Shwetha Narasimhan ◽  
Andreas Primeßnig ◽  
Irina Kaverina ◽  
Florian Rehfeldt
Keyword(s):  
Extracellular Matrix ◽  
Quantitative Analysis ◽  
Focal Adhesion ◽  
Cross Correlation ◽  
Focal Adhesions ◽  
Traction Force ◽  
Stress Fibers ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Actin Stress Fibers

AbstractFocal adhesions (FAs) and associated actin stress fibers (SFs) form a complex mechanical system that mediates bidirectional interactions between cells and their environment. This linked network is essential for mechanosensing, force production and force transduction, thus directly governing cellular processes like polarization, migration and extracellular matrix remodeling. We introduce a tool for fast and robust coupled analysis of both FAs and SFs named the Focal Adhesion Filament Cross-correlation Kit (FAFCK). Our software can detect and record location, axes lengths, area, orientation, and aspect ratio of focal adhesion structures as well as the location, length, width and orientation of actin stress fibers. This enables users to automate analysis of the correlation of FAs and SFs and study the stress fiber system in a higher degree, pivotal to accurately evaluate transmission of mechanocellular forces between a cell and its surroundings. The FAFCK is particularly suited for unbiased and systematic quantitative analysis of FAs and SFs necessary for novel approaches of traction force microscopy that uses the additional data from the cellular side to calculate the stress distribution in the substrate. For validation and comparison with other tools, we provide datasets of cells of varying quality that are labelled by a human expert. Datasets and FAFCK are freely available as open source under the GNU General Public License.Author summaryOur novel Focal Adhesion Filament Cross-correlation Kit (FAFCK) allows for fast, reliable, unbiased, and systematic detection of focal adhesions and actin stress fibers in cells and their mutual correlation. Detailed analysis of these structures which are both key elements in mechano-sensing and force transduction will help tremendously to improve quantitative analysis of mechanocellular experiments, key to understanding the complex interplay between cells and the extracellular matrix. In particular, sophisticated analysis methods such as model-based traction force microscopy will benefit from correlating the detailed datasets of stress fibers and focal adhesions.

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