scholarly journals Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shuangtao Li ◽  
Lei Zhang ◽  
Ying Wang ◽  
Fengfeng Xu ◽  
Mengyun Liu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Leaf Anatomy ◽  
Cellulose Synthase ◽  
Cellulose Content ◽  
Cellulose Synthase Gene

scholarly journals Gibberellin induced transcription factor bZIP53 regulates CesA1 expression in maize kernels

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0244591
Author(s):  
Huayang Lv ◽  
Xiao Li ◽  
Hui Li ◽  
Yufeng Hu ◽  
Hanmei Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Cellulose Synthase ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Hormone Regulation ◽  
Grain Development ◽  
Cellulose Synthase Gene ◽  
Maize Grain ◽  
Maize Kernels

Proper development of the maize kernel is of great significance for high and stable maize yield to ensure national food security. Gibberellin (GA), one of the hormones regulating plant growth, is involved in modulating the development of maize kernels. Cellulose, one of the main components of plant cells, is also regulated by gibberellin. The mechanism of hormone regulation during maize grain development is highly complicated, and reports on GA-mediated modulation of cellulose synthesis during maize grain development are rare. Our study revealed that during grain growth and development, the grain length and bulk density of GA-treated corn kernels improved significantly, and the cellulose content of grains increased, while seed coat thickness decreased. The transcription factor basic region/leucine zipper motif 53 (bZIP53), which is strongly correlated with cellulose synthase gene 1 (CesA1) expression, was screened by transcriptome sequencing and the expression of the cellulose synthase gene in maize grain development after GA treatment was determined. It was found that bZIP53 expression significantly promoted the expression of CesA1. Further, analysis of the transcription factor bZIP53 determined that the gene-encoded protein was localized in the cell and nuclear membranes, but the transcription factor bZIP53 itself showed no transcriptional activation. Further studies are required to explore the interaction of bZIP53 with CesA1.

scholarly journals Arabidopsis heat shock transcription factor HSFA7b positively mediates salt stress tolerance by binding to an E-box-like motif to regulate gene expression

2019 ◽  
Vol 70 (19) ◽  
pp. 5355-5374 ◽  
Author(s):  
Dandan Zang ◽  
Jingxin Wang ◽  
Xin Zhang ◽  
Zhujun Liu ◽  
Yucheng Wang
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Heat Shock ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Ion Homeostasis ◽  
Cellulose Synthase ◽  
Regulate Gene Expression ◽  
E Box ◽  
Regulate Gene

Abstract Plant heat shock transcription factors (HSFs) are involved in heat and other abiotic stress responses. However, their functions in salt tolerance are little known. In this study, we characterized the function of a HSF from Arabidopsis, AtHSFA7b, in salt tolerance. AtHSFA7b is a nuclear protein with transactivation activity. ChIP-seq combined with an RNA-seq assay indicated that AtHSFA7b preferentially binds to a novel cis-acting element, termed the E-box-like motif, to regulate gene expression; it also binds to the heat shock element motif. Under salt conditions, AtHSFA7b regulates its target genes to mediate serial physiological changes, including maintaining cellular ion homeostasis, reducing water loss rate, decreasing reactive oxygen species accumulation, and adjusting osmotic potential, which ultimately leads to improved salt tolerance. Additionally, most cellulose synthase-like (CSL) and cellulose synthase (CESA) family genes were inhibited by AtHSFA7b; some of them were randomly selected for salt tolerance characterization, and they were mainly found to negatively modulate salt tolerance. By contrast, some transcription factors (TFs) were induced by AtHSFA7b; among them, we randomly identified six TFs that positively regulate salt tolerance. Thus, AtHSFA7b serves as a transactivator that positively mediates salinity tolerance mainly through binding to the E-box-like motif to regulate gene expression.

Virus-Induced Silencing of a Plant Cellulose Synthase Gene

2000 ◽  
Vol 12 (5) ◽  
pp. 691 ◽  
Author(s):  
Rachel A. Burton ◽  
David M. Gibeaut ◽  
Antony Bacic ◽  
Kim Findlay ◽  
Keith Roberts ◽  
...  
Keyword(s):  
Cellulose Synthase ◽  
Cellulose Synthase Gene

scholarly journals In silico and functional characterization of the promoter of a Eucalyptussecondary cell wall associated cellulose synthase gene (EgCesA1)

2011 ◽  
Vol 5 (S7) ◽  
Author(s):  
Nicky Creux ◽  
Minique De Castro ◽  
Martin Ranik ◽  
Antanas Spokevicius ◽  
Gerd Bossinger ◽  
...  
Keyword(s):  
Cell Wall ◽  
In Silico ◽  
Functional Characterization ◽  
Cellulose Synthase ◽  
Cellulose Synthase Gene

scholarly journals A semi-dominant mutation in OsCESA9 improves biomass enzymatic digestibility and salt tolerance by relatively remodeling cell walls in rice

2020 ◽  
Author(s):  
Yafeng Ye ◽  
Shuoxun Wang ◽  
Kun Wu ◽  
Yan Ren ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Cellulose Content ◽  
Normal Plant ◽  
Wild Type ◽  
Negative Effects ◽  
Straw Return ◽  
Almost All

Abstract Background: Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another. Result: Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type (WT). Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging. Conclusion: Hence, manipulation of OsCESA9D387N can provide the perspective of the rice straw for biofuels and bioproducts due to its improved enzymatic saccharification.

scholarly journals Disruption of Very-Long-Chain-Fatty Acid Synthesis Has an Impact on the Dynamics of Cellulose Synthase in Arabidopsis thaliana

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1599
Author(s):  
Xiaoyu Zhu ◽  
Frédérique Tellier ◽  
Ying Gu ◽  
Shundai Li
Keyword(s):  
Fatty Acid ◽  
Cell Biology ◽  
Higher Plants ◽  
Threshold Level ◽  
Cellulose Synthase ◽  
Chain Fatty Acid ◽  
Cellulose Synthesis ◽  
Cellulose Content ◽  
Long Chain Fatty Acid ◽  
Long Chain

In higher plants, cellulose is synthesized by membrane-spanning large protein complexes named cellulose synthase complexes (CSCs). In this study, the Arabidopsis PASTICCINO2 (PAS2) was identified as an interacting partner of cellulose synthases. PAS2 was previously characterized as the plant 3-hydroxy-acyl-CoA dehydratase, an ER membrane-localized dehydratase that is essential for very-long-chain-fatty acid (VLCFA) elongation. The pas2-1 mutants show defective cell elongation and reduction in cellulose content in both etiolated hypocotyls and light-grown roots. Although disruption of VLCFA synthesis by a genetic alteration had a reduction in VLCFA in both etiolated hypocotyls and light-grown roots, it had a differential effect on cellulose content in the two systems, suggesting the threshold level of VLCFA for efficient cellulose synthesis may be different in the two biological systems. pas2-1 had a reduction in both CSC delivery rate and CSC velocity at the PM in etiolated hypocotyls. Interestingly, Golgi but not post-Golgi endomembrane structures exhibited a severe defect in motility. Experiments using pharmacological perturbation of VLCFA content in etiolated hypocotyls strongly indicate a novel function of PAS2 in the regulation of CSC and Golgi motility. Through a combination of genetic, biochemical and cell biology studies, our study demonstrated that PAS2 as a multifunction protein has an important role in the regulation of cellulose biosynthesis in Arabidopsis hypocotyl.

scholarly journals The cellulose synthase gene of Dictyostelium

2000 ◽  
Vol 97 (5) ◽  
pp. 2391-2396 ◽  
Author(s):  
R. L. Blanton ◽  
D. Fuller ◽  
N. Iranfar ◽  
M. J. Grimson ◽  
W. F. Loomis
Keyword(s):  
Cellulose Synthase ◽  
Cellulose Synthase Gene

scholarly journals Revised Phylogeny of the Cellulose Synthase Gene Superfamily: Insights into Cell Wall Evolution

2018 ◽  
Vol 177 (3) ◽  
pp. 1124-1141 ◽  
Author(s):  
Alan Little ◽  
Julian G. Schwerdt ◽  
Neil J. Shirley ◽  
Shi F. Khor ◽  
Kylie Neumann ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cellulose Synthase ◽  
Cellulose Synthase Gene ◽  
Cellulose Synthase Gene Superfamily
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