scholarly journals PrimerSuite: A High-Throughput Web-Based Primer Design Program for Multiplex Bisulfite PCR

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jennifer Lu ◽  
Andrew Johnston ◽  
Philippe Berichon ◽  
Ke-lin Ru ◽  
Darren Korbie ◽  
...  
Keyword(s):  
High Throughput ◽  
Primer Design ◽  
Web Based ◽  
Design Program ◽  
Primer Design Program

scholarly journals Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions

2010 ◽  
Vol 3 (1) ◽  
Author(s):  
Andrew MK Brown ◽  
Ken Sin Lo ◽  
Paul Guelpa ◽  
Mélissa Beaudoin ◽  
John D Rioux ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Primer Design ◽  
Next Generation ◽  
Design Program ◽  
Primer Design Program ◽  
Generation Sequencing

PRIMO: A Primer Design Program That Applies Base Quality Statistics for Automated Large-Scale DNA Sequencing

Genomics ◽  
1997 ◽  
Vol 40 (3) ◽  
pp. 476-485 ◽  
Author(s):  
Ping Li ◽  
Kenneth C. Kupfer ◽  
Christopher J. Davies ◽  
David Burbee ◽  
Glen A. Evans ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Large Scale ◽  
Primer Design ◽  
Design Program ◽  
Primer Design Program

scholarly journals Enhancements and modifications of primer design program Primer3

2007 ◽  
Vol 23 (10) ◽  
pp. 1289-1291 ◽  
Author(s):  
T. Koressaar ◽  
M. Remm
Keyword(s):  
Primer Design ◽  
Design Program ◽  
Primer Design Program

scholarly journals SNPbox: web-based high-throughput primer design from gene to genome

2004 ◽  
Vol 32 (Web Server) ◽  
pp. W170-W172 ◽  
Author(s):  
S. Weckx ◽  
P. De Rijk ◽  
C. Van Broeckhoven ◽  
J. Del-Favero
Keyword(s):  
High Throughput ◽  
Primer Design ◽  
Web Based

Development of UPSR Primer Design Program for Efficient Detection of Viruses

2008 ◽  
Vol 38 (4) ◽  
pp. 259 ◽  
Author(s):  
So Young Jang ◽  
Hyung Woo Yoon ◽  
Sung Ae Oh ◽  
Keon Myung Lee ◽  
Chan Hee Lee
Keyword(s):  
Primer Design ◽  
Efficient Detection ◽  
Design Program ◽  
Primer Design Program

scholarly journals Analisis Mutasi pada Kodon 531 Pada Gen Rpob Mycobacterium tuberculosis Penyebab Resistensi Rifampisin

2017 ◽  
Vol 15 (2) ◽  
pp. 140
Author(s):  
Yatnita Parama Cita ◽  
Dwi Hilda Putri
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Bacterial Resistance ◽  
Rpob Gene ◽  
Resistance Mechanisms ◽  
Primer Design ◽  
Reference Sequence ◽  
Multi Drug Resistance ◽  
Accession Number ◽  
Chemotherapy Agent ◽  
Primer Design Program

Tuberculosis (TB) is a serious disesase in the world. According to the WHO, it is estimated more than 3 million people die every year as a result of this infectious disease. One factor that causes diffi culty handling TB chemoteraphy is not effective against the bacteria Mycobacterium tuberculosis that causes TB . Effectiveness of treatment is often hampered by the emergence of bacterial resistance against M. Tuberculosis chemotherapy agents are given. From some research found that bacterial resistance may occur in more one type of chemotherapy agent also known as multi-drug resistance (MDR). Mycobacterium tuberculosis develop resistance mechanisms that are different from other bacteria in general. In prokaryotes, resistance is generally due to the transfer of genetic, either through plasmids,transposons and other. Reference sequence beta sub unit of RNAP protein M. Tuberculosis with accession number NP_215181.1 and M. tucerculosis rpoB gene with accession number NC_000962.3 used to obtain preliminary information from the data base www.ncbi.nlm.gov and www.uniprot.org . Mutation done according to several studies literature. Analysis of the composition, profi le, location and structure of protein using www.expasy.org, TMHMM and http://bioinf.cs.ucl.ac.uk/psipred. The primer design is done with Primer Design Program. Based on the analysis of mutation in the beta subunit of RNAP protein M. Tuberculosis, codon 531 (Ser ->Leu), it is known that mutations cause changes in some properties and structure of proteins. Possible changes affecting the nature of bacterial resistance to antibiotics rifampicin. However, further analysis needs to be done with the analysis of the docking technique.

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