scholarly journals A sensitive three monoclonal antibodies based automatic latex particle-enhanced turbidimetric immunoassay for Golgi protein 73 detection

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yanyan Xia ◽  
Han Shen ◽  
Yefei Zhu ◽  
Hongpan Xu ◽  
Zhiyang Li ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Latex Particle ◽  
Golgi Protein ◽  
Turbidimetric Immunoassay

AB1236 KL-6 AS A BIOMARKER FOR SJÖGREN SYNDROME-ASSOCIATED INTERSTITIAL LUNG DISEASE: METHODOLOGY MATTERS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1908.1-1909
Author(s):  
Y. H. Chiu ◽  
C. C. Lu ◽  
F. C. Liu ◽  
S. E. Tang ◽  
S. J. Chu ◽  
...  
Keyword(s):  
Interstitial Lung Disease ◽  
Lung Disease ◽  
Latex Particle ◽  
Sjögren Syndrome ◽  
Lung Fibroblasts ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sjogren Syndrome ◽  
Predictive Values ◽  
Muc1 Mucin ◽  
Turbidimetric Immunoassay

Background:Krebs von den Lungen-6 (KL-6) was recently found to be a serum biomarker for various disease associated interstitial lung disease (ILD) including primary Sjögren syndrome (pSS).1KL-6 is a high-molecular-weight glycoprotein in the Mucin 1 protein group and is majorly expressed by regenerating type II pneumocytes; hence, serum KL-6 levels may reflect the severity of pulmonary damage with regeneration.2Human KL-6 also promotes the proliferation and survival of pulmonary fibroblasts and the differentiation of myofibroblasts, which enhance fibrosis.3, 4Objectives:To evaluate the agreement between the latex particle-enhanced turbidimetric immunoassay with enzyme-linked immunosorbent assay (ELISA) and association with clinical phenotypes.Methods:This retrospective case-control study included 39 patients with pSS, of whom 21 (53.85%) patients developed ILD at the end of follow-up. The serum KL-6 level was compared between latex particle-enhanced turbidimetric immunoassay (Nanopia) and ELISA (MBS2601395; MyBioSource, CA, USA). Electronic medical records were reviewed, including clinical information, images, pulmonary function test, and laboratory results on inclusion, and a chest physician reviewed the results of pulmonary radiography.Results:The two serum KL-6 immunoassays revealed a moderate correlation with a Pearson product-moment correlation coefficient of 0.427. Serum KL-6 levels, measured using ELISA, were 1920.10 ± 1974.26 U/mL and 894.11 ± 788.53 U/mL in the ILD and non-ILD groups, respectively (p = 0.001). The latex particle-enhanced turbidimetric immunoassay for serum KL-6 was 459.62 ± 331.41 U/mL and 265.33 ± 105.37 U/mL in the ILD and non-ILD group, respectively (p = 0.074). The predictive values of serum KL-6 in the area under the receiver-operating characteristic curve were 0.810 and 0.669 in ELISA and latex particle-enhanced turbidimetric immunoassay, respectively.Conclusion:Serum KL-6 is a predicting biomarker in pSS patients who may develop ILD. However, the methodology of immunoassay may influence the efficacy of the prediction and clinical association.References:[1]Chiu Y-H, Lu C-C, Liu F-C, et al. FRI0228 KL-6 AS A BIOMARKER OF DEVELOPING INTERSTITIAL LUNG DISEASE IN PATIENTS WITH SJÖGREN SYNDROME.Ann Rheum Dis. 2019; 78: 793.[2]Yousefi M, Dehghani S, Nosrati R, et al. Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review.Biosensors & bioelectronics. 2019; 130: 1-19.[3]Xu L, Yan DR, Zhu SL, et al. KL-6 regulated the expression of HGF, collagen and myofibroblast differentiation.Eur Rev Med Pharmacol Sci. 2013; 17: 3073-7.[4]Ohshimo S, Yokoyama A, Hattori N, Ishikawa N, Hirasawa Y and Kohno N. KL-6, a human MUC1 mucin, promotes proliferation and survival of lung fibroblasts.Biochem Biophys Res Commun. 2005; 338: 1845-52.Acknowledgments:This work was supported by National Defense Medical Center and Tri-Service General Hospital (TSGH-D-109183). The authors thank Kuo’s Yuan In Enterprise co., LTD for supporting Nanopia KL-6 Kit.Disclosure of Interests:None declared

Stabilization of turbidimetric immunoassay by covalent coupling of antibody to latex particles

1991 ◽  
Vol 37 (7) ◽  
pp. 1248-1251 ◽  
Author(s):  
H Thakkar ◽  
C L Davey ◽  
E A Medcalf ◽  
L Skingle ◽  
A R Craig ◽  
...  
Keyword(s):  
Latex Particle ◽  
Serum Proteins ◽  
Assay Sensitivity ◽  
Latex Particles ◽  
Covalent Coupling ◽  
Signal Change ◽  
Analytical Recovery ◽  
Turbidimetric Assay ◽  
High Concentrations ◽  
Turbidimetric Immunoassay

Abstract Turbidimetric immunoassay is commonly used to quantify serum proteins. Latex-particle enhancement of this type of assay has been primarily associated with increasing assay sensitivity. However, covalent coupling of an antibody to a latex particle can offer other advantages that are also pertinent in measurement of high concentrations of analytes. By using a common antibody with IgG as a model analyte, we describe the development of a nonenhanced and a latex-particle-enhanced turbidimetric assay for measuring serum IgG. Both assays show adequate analytical recovery and parallelism, and results compare well with those by rate nephelometry. The latex-enhanced assay has equivalent sensitivity, working range, and interassay precision, but much greater signal change and calibration stability than the nonenhanced assay. In addition, with latex particles, less antiserum is needed. Coupling antibodies to latex particles offers considerable advantages, even when an improved assay detection limit is not required.

scholarly journals Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer

2015 ◽  
Vol 442 ◽  
pp. 130-135 ◽  
Author(s):  
Tetsuo Machida ◽  
Kazuya Miyashita ◽  
Takuya Sone ◽  
Sayori Tanaka ◽  
Katsuyuki Nakajima ◽  
...  
Keyword(s):  
Lipoprotein Lipase ◽  
Latex Particle ◽  
Serum Lipoprotein ◽  
Turbidimetric Immunoassay

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

Structural and Chemical Studies of Pathological Fibers in Human Neurofibrillary Degeneration

1985 ◽  
Vol 43 ◽  
pp. 726-729
Author(s):  
K.S. Kosik ◽  
L.K. Duffy ◽  
S. Bakalis ◽  
C. Abraham ◽  
D.J. Selkoe
Keyword(s):  
Monoclonal Antibodies ◽  
Alzheimer Disease ◽  
Human Brain ◽  
Neurofibrillary Tangles ◽  
Morphological Analysis ◽  
High Specificity ◽  
Cytoskeletal Proteins ◽  
Neuritic Plaque ◽  
Protein Chemistry ◽  
Chemical Studies

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.

Use of immunoblotting and monoclonal antibodies to evaluate the residual antigenic activity of milk protein hydrolysed formulae

1996 ◽  
Vol 26 (10) ◽  
pp. 1182-1187 ◽  
Author(s):  
P. RESTANI ◽  
A. PLEBANI ◽  
T. VELONA ◽  
G. CAVAGNI ◽  
A. G. UGAZIO ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Milk Protein ◽  
Antigenic Activity

Monoclonal Antibodies for Ca, Asthma, and RA

Ob Gyn News ◽  
2008 ◽  
Vol 43 (4) ◽  
pp. 12 ◽  
Author(s):  
GERALD G. BRIGGS
Keyword(s):  
Monoclonal Antibodies
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