scholarly journals Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Francisco Abadía-Molina ◽  
Virginia Morón-Calvente ◽  
Stephen D. Baird ◽  
Fahad Shamim ◽  
Francisco Martín ◽  
...  
Keyword(s):  
Cell Division ◽  
Neuronal Apoptosis ◽  
Inhibitory Protein ◽  
Neuronal Apoptosis Inhibitory Protein

scholarly journals Induction of neuronal apoptosis inhibitory protein expression in response to androgen deprivation in prostate cancer

2010 ◽  
Vol 292 (2) ◽  
pp. 176-185 ◽  
Author(s):  
Helen H.L. Chiu ◽  
Theresa M.K. Yong ◽  
Jun Wang ◽  
Yuwei Wang ◽  
Robert L. Vessella ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Protein Expression ◽  
Neuronal Apoptosis ◽  
Androgen Deprivation ◽  
Inhibitory Protein ◽  
Neuronal Apoptosis Inhibitory Protein

scholarly journals A Dopamine D4 Receptor Antagonist Attenuates Ischemia-Induced Neuronal Cell Damage via Upregulation of Neuronal Apoptosis Inhibitory Protein

2005 ◽  
Vol 25 (7) ◽  
pp. 794-806 ◽  
Author(s):  
Yoshinori Okada ◽  
Harumi Sakai ◽  
Eri Kohiki ◽  
Etsuko Suga ◽  
Yoshiko Yanagisawa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Neuronal Apoptosis ◽  
Neuronal Cell ◽  
Dopamine D4 Receptor ◽  
Inhibitory Protein ◽  
Ca1 Neurons ◽  
Hippocampal Ca1 ◽  
Neuronal Apoptosis Inhibitory Protein ◽  
D4 Receptor

Neuronal apoptosis inhibitory protein (NAIP/BIRC1), the inhibitor of apoptosis protein (IAP) family member, suppresses neuronal cell death induced by a variety of insults, including cell death from ischemia and stroke. The goal of the present study was to develop an efficient method for identification of compounds with the ability to upregulate endogenous NAIP and to determine the effects on these compounds on the cellular response to ischemia. A novel NAIP-enzyme-linked immunosorbent assay (ELISA)-based in vitro drug-screening system is established. Use of this system identified an antagonist of dopamine D4 receptor, termed L-745,870, with a potent NAIP upregulatory effect. L-745,870-mediated NAIP upregulation in neuronal and nonneuronal cultured cells resulted in decreased vulnerability to oxidative stress-induced apoptosis. Reducing NAIP expression via RNA interference techniques resulted in prevention of L-745,870-mediated protection from oxidative stress. Further, systemic administration of L-745,870 attenuated ischemia-induced damage of the hippocampal CA1 neurons and upregulated NAIP expression in the rescued hippocampal CA1 neurons in a gerbil model. These data suggest that the NAIP upregulating compound, L-745,870, has therapeutic potential in acute ischemic disorders and that our NAIP-ELISA-based drug screening may facilitate the discovery of novel neuroprotective compounds.

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