scholarly journals Pentacle gold–copper alloy nanocrystals: a new system for entering male germ cells in vitro and in vivo

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu Lin ◽  
Rong He ◽  
Liping Sun ◽  
Yushan Yang ◽  
Wenqing Li ◽  
...  
Keyword(s):  
Germ Cells ◽  
Copper Alloy ◽  
Male Germ Cells ◽  
Alloy Nanocrystals ◽  
New System

scholarly journals Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study

2012 ◽  
Vol 14 (4) ◽  
pp. 574-579 ◽  
Author(s):  
Yong Zhu ◽  
Hong-Liang Hu ◽  
Peng Li ◽  
Shi Yang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Ips Cells ◽  
In Vivo Study ◽  
Male Germ Cells ◽  
Induced Pluripotent

scholarly journals Partial Sperm beta1 Integrin Subunit Deletion Proves Its Involvement in Mouse Gamete Adhesion/Fusion

2020 ◽  
Vol 21 (22) ◽  
pp. 8494
Author(s):  
Virginie Barraud-Lange ◽  
Côme Ialy-Radio ◽  
Céline Chalas ◽  
Isabelle Holtzmann ◽  
Jean-Philippe Wolf ◽  
...  
Keyword(s):  
Western Blot ◽  
Germ Cells ◽  
Blot Analysis ◽  
Integrin Subunit ◽  
Perivitelline Space ◽  
Male Germ Cells ◽  
Beta1 Integrin ◽  
First Time

We have previously shown, using antibodies, that the sperm alpha6beta1 integrin is involved in mouse gamete fusion in vitro. Here we report the conditional knockdown of the sperm Itgb1 gene. It induced a drastic failure of sperm fusogenic ability with sperm accumulation in the perivitelline space of in vitro inseminated oocytes deleted or not for the Itgb1 gene. These data demonstrate that sperm, but not oocyte, beta1 integrin subunit is involved in gamete adhesion/fusion. Curiously, knockdown males were fertile in vivo probably because of the incomplete Cre-mediated deletion of the sperm Itgb1 floxed gene. Indeed, this was shown by Western blot analysis and confirmed by both the viability and litter size of pups obtained by mating partially sperm Itgb1 deleted males with females producing completely deleted Itgb1 oocytes. Because of the total peri-implantation lethality of Itgb1 deletion in mice, we assume that sperm that escaped the Itgb1 excision seemed to be preferentially used to fertilize in vivo. Here, we showed for the first time that the deletion, even partial, of the sperm Itgb1 gene makes the sperm unable to normally fertilize oocytes. However, to elucidate the question of the essentiality of its role during fertilization, further investigations using a mouse expressing a recombinase more effective in male germ cells are necessary.

scholarly journals Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 745 ◽  
Author(s):  
Fahar Ibtisham ◽  
Ali Honaramooz
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Recent Progress ◽  
Male Germ Cells ◽  
Culture Systems ◽  
In Vitro Spermatogenesis ◽  
Haploid Male ◽  
Restoration Of Fertility

Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes onto the next generation. SSCs also have the potential to provide important knowledge about stem cells in general and to offer critical in vitro and in vivo applications in assisted reproductive technologies. After century-long research, proof-of-principle culture systems have been introduced to support the in vitro differentiation of SSCs from rodent models into haploid male germ cells. Despite recent progress in organotypic testicular tissue culture and two-dimensional or three-dimensional cell culture systems, to achieve complete in vitro spermatogenesis (IVS) using non-rodent species remains challenging. Successful in vitro production of human haploid male germ cells will foster hopes of preserving the fertility potential of prepubertal cancer patients who frequently face infertility due to the gonadotoxic side-effects of cancer treatment. Moreover, the development of optimal systems for IVS would allow designing experiments that are otherwise difficult or impossible to be performed directly in vivo, such as genetic manipulation of germ cells or correction of genetic disorders. This review outlines the recent progress in the use of SSCs for IVS and potential in vivo applications for the restoration of fertility.

scholarly journals Gonadal sex reversal of the developing marsupial ovary in vivo and in vitro

Development ◽  
1996 ◽  
Vol 122 (12) ◽  
pp. 4057-4063 ◽  
Author(s):  
D.J. Whitworth ◽  
G. Shaw ◽  
M.B. Renfree
Keyword(s):  
Germ Cells ◽  
Normal Development ◽  
Tammar Wallaby ◽  
Mitotic Division ◽  
Sex Reversal ◽  
Direct Result ◽  
Male Germ Cells ◽  
Well Differentiated

Undifferentiated tammar wallaby ovaries were transplanted under the skin of male pouch young during the period of mitotic division of the XX germ cells. After 25 days, all the germ cells had disappeared and the ovaries contained seminiferous-like cords. Similarly, undifferentiated ovaries cultured for 4 days with recombinant human Mullerian-inhibiting substance (rhMIS) also contained well-differentiated seminiferous-like cords and few or no surviving germ cells. The majority of controls cultured without rhMIS developed as normal ovaries. However, in a few control ovaries seminiferous-like cords developed in those regions of the ovaries that were partially necrotic and contained few germ cells. These results strongly suggest that sex-reversal of the tammar ovary is the direct result of loss of mitotic germ cells, rather than an effect of MIS on female somatic cells. MIS is apparently toxic to these female germ cells in mitosis, but not to male germ cells in mitosis. Thus, in normal development in the tammar, the presence of XX germ cells in the ovary inhibits the formation of seminiferous cords so that the gonad develops as an ovary.

scholarly journals Analysis ofSPATA19gene expression during male germ cells development, lessons from in vivo and in vitro study

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Seyedmehdi Nourashrafeddin ◽  
Reza Ebrahimzadeh-Vesal ◽  
Mohammad Miryounesi ◽  
Mahmoud Aarabi ◽  
Nosratollah Zarghami ◽  
...  
Keyword(s):  
Germ Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Male Germ Cells

scholarly journals In vivo and in vitro Radioautographic Investigation of Amino Acid Incorporation into Male Germ Cells

1981 ◽  
Vol 24 (1) ◽  
pp. 153-162 ◽  
Author(s):  
J. P. Dadoune ◽  
M. A. Fain-maurel ◽  
M. F. Alfonsi ◽  
G. Katsanis
Keyword(s):  
Amino Acid ◽  
Germ Cells ◽  
Amino Acid Incorporation ◽  
Male Germ Cells ◽  
Acid Incorporation

Birth of healthy twins after fertilization with in vitro cultured spermatids from a patient with massive in vivo apoptosis of postmeiotic germ cells

2000 ◽  
Vol 74 (5) ◽  
pp. 1044-1046 ◽  
Author(s):  
Jan Tesarik ◽  
Natalio Cruz-Navarro ◽  
Eduardo Moreno ◽  
Maria Teresa Cañete ◽  
Carmen Mendoza
Keyword(s):  
Germ Cells
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