scholarly journals Global proteomic analysis of prenylated proteins in Plasmodium falciparum using an alkyne-modified isoprenoid analogue

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kiall F. Suazo ◽  
Chad Schaber ◽  
Charuta C. Palsuledesai ◽  
Audrey R. Odom John ◽  
Mark D. Distefano
Keyword(s):  
Plasmodium Falciparum ◽  
Proteomic Analysis ◽  
Prenylated Proteins

scholarly journals Proteomic analysis of Plasmodium falciparum histone deacetylase 1 complex proteins

2019 ◽  
Vol 198 ◽  
pp. 7-16 ◽  
Author(s):  
Jessica A. Engel ◽  
Emma L. Norris ◽  
Paul Gilson ◽  
Jude Przyborski ◽  
Addmore Shonhai ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Histone Deacetylase ◽  
Proteomic Analysis ◽  
Histone Deacetylase 1

scholarly journals Proteomic analysis of Plasmodium falciparum parasites from patients with cerebral and uncomplicated malaria

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Gwladys I. Bertin ◽  
Audrey Sabbagh ◽  
Nicolas Argy ◽  
Virginie Salnot ◽  
Sem Ezinmegnon ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Proteomic Analysis ◽  
Uncomplicated Malaria

scholarly journals Isoprenoid Biosynthesis Inhibition Disrupts Rab5 Localization and Food Vacuolar Integrity in Plasmodium falciparum

2012 ◽  
Vol 12 (2) ◽  
pp. 215-223 ◽  
Author(s):  
Ruth Howe ◽  
Megan Kelly ◽  
John Jimah ◽  
Dana Hodge ◽  
Audrey R. Odom
Keyword(s):  
Plasmodium Falciparum ◽  
De Novo ◽  
Small Gtpases ◽  
Isoprenoid Biosynthesis ◽  
Vesicular Trafficking ◽  
Mep Pathway ◽  
Protein Prenylation ◽  
Content Type ◽  
Pathway Inhibition ◽  
Prenylated Proteins

ABSTRACT The antimalarial agent fosmidomycin is a validated inhibitor of the nonmevalonate isoprenoid biosynthesis (methylerythritol 4-phosphate [MEP]) pathway in the malaria parasite, Plasmodium falciparum . Since multiple classes of prenyltransferase inhibitors kill P. falciparum , we hypothesized that protein prenylation was one of the essential functions of this pathway. We found that MEP pathway inhibition with fosmidomycin reduces protein prenylation, confirming that de novo isoprenoid biosynthesis produces the isoprenyl substrates for protein prenylation. One important group of prenylated proteins is small GTPases, such as Rab family members, which mediate cellular vesicular trafficking. We have found that Rab5 proteins dramatically mislocalize upon fosmidomycin treatment, consistent with a loss of protein prenylation. Fosmidomycin treatment caused marked defects in food vacuolar morphology and integrity, consistent with a defect in Rab-mediated vesicular trafficking. These results provide insights to the biological functions of isoprenoids in malaria parasites and may assist the rational selection of secondary agents that will be useful in combination therapy with new isoprenoid biosynthesis inhibitors.

scholarly journals Proteomic analysis of Plasmodium falciparum response to isocryptolepine derivative

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0220871 ◽  
Author(s):  
Kitiya Rujimongkon ◽  
Mathirut Mungthin ◽  
Jumreang Tummatorn ◽  
Sumate Ampawong ◽  
Poom Adisakwattana ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Proteomic Analysis

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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