scholarly journals Caffeine prevents kidney stone formation by translocation of apical surface annexin A1 crystal-binding protein into cytoplasm: In vitro evidence

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Paleerath Peerapen ◽  
Visith Thongboonkerd
Keyword(s):  
Kidney Stone ◽  
Binding Protein ◽  
Stone Formation ◽  
Apical Surface ◽  
Annexin A1 ◽  
Crystal Binding ◽  
Kidney Stone Formation

scholarly journals Effect of theCopaifera langsdorffiiDesf. Leaf Extract on the Ethylene Glycol-Induced Nephrolithiasis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Rejane Barbosa de Oliveira ◽  
Eduardo Barbosa Coelho ◽  
Marina Rezende Rodrigues ◽  
Ana Rita de Mello Costa-Machado ◽  
João Paulo Barreto de Sousa ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Kidney Stone ◽  
Biochemical Parameters ◽  
Leaf Extract ◽  
Stone Formation ◽  
Osteopontin Expression ◽  
Copaifera Langsdorffii ◽  
Kidney Stone Formation ◽  
Different Doses

The potential of theCopaifera langsdorffiileaves extract to prevent stone formation was analyzed by means of an ethylene glycol (EG) animal model of nephrolithiasis and anin vitrocrystallization assay. Different doses of theC. langsdorffiileaves extract were administered to rats treated with EG. Urine biochemical parameters were quantified. CaOx deposits count and analysis of osteopontin expression were conducted on kidneys fixed in formalin. Thein vitroassay was performed by turbidimetry. Phytochemical analyses of the extract were accomplished by HPLC-UV-DAD, and several compounds were isolated.C. langsdorffiileaf extract was able to avoid stone formation. The number of deposits was50.30±31.29at the higher extract dose, compared to the value of179.5±45.96achieved with the EG control. Significantly lower oxalate levels and OPN expression and increased citrate levels were observed after extract administration. In thein vitroassay, the extract diluted the formed crystals. Phytochemical analyses showed that the extract is rich in phenolic compounds that are capable of preventing stone formation. Thus, on the basis of our results, we suggest that theC. langsdorffiileaf extract has potential application in the prevention of kidney stone formation.

Effects of inter-α-inhibitor and several of its derivatives on calcium oxalate crystallization in vitro

2000 ◽  
Vol 98 (4) ◽  
pp. 471-480 ◽  
Author(s):  
Caroline DEAN ◽  
Jerry KANELLOS ◽  
Hung PHAM ◽  
Maria GOMES ◽  
Adrian OATES ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Kidney Stone ◽  
Stone Formation ◽  
Calcium Oxalate Crystallization ◽  
Sds Page ◽  
Kidney Stone Formation ◽  
Scanning Electron

The bikunin peptide chain of the protease inhibitor inter-α-inhibitor (∣α∣) has been reported to be an inhibitor of calcium oxalate (CaOx) crystallization, and hence has been proposed as having a role in CaOx kidney stone formation. However, further experimental evidence is required to assess if fragments of ∣α∣ other than bikunin may play a role in the regulation of crystallization events in stone formation. The aim of the present study was to assess the effects of ∣α∣ and several of its derivatives on CaOx crystallization in a seeded inorganic system and to compare these effects with those of a known inhibitor of crystallization, prothrombin. ∣α∣ was purified from a preparation of human plasma and fragmented by alkaline hydrolysis, and two of its peptide chains, bikunin and heavy chain 1 (H1), were purified further by HPLC. Their purity was confirmed by SDS/PAGE. Using Coulter counter and [14C]oxalate analysis and scanning electron microscopy, ∣α∣, its H1 chain and bikunin from urine and from plasma were shown to be relatively weak inhibitors of CaOx crystallization in vitro at expected physiological concentrations. It was concluded that members of the ∣α∣ family may not be as important in kidney stone formation as has been generally proposed, although further studies are required before a possible role for ∣α∣ and its fragments in stone formation can be unambiguously discounted.

In vitro anti-lithogenic activity of lime powder regimen (LPR) and the effect of LPR on urinary risk factors for kidney stone formation in healthy volunteers

Urolithiasis ◽  
2015 ◽  
Vol 43 (2) ◽  
pp. 125-134 ◽  
Author(s):  
Pajaree Chariyavilaskul ◽  
Poonsin Poungpairoj ◽  
Suchada Chaisawadi ◽  
Chanchai Boonla ◽  
Thasinas Dissayabutra ◽  
...  
Keyword(s):  
Risk Factors ◽  
Healthy Volunteers ◽  
Kidney Stone ◽  
Stone Formation ◽  
Lime Powder ◽  
Kidney Stone Formation

2086 RENAL MACROPHAGES COULD ENGLOBE CALCIUM OXALATE CRYSTALS DURING KIDNEY STONE FORMATION IN VITRO AND IN VIVO MODELS

2012 ◽  
Vol 187 (4S) ◽  
Author(s):  
Atsushi Okada ◽  
Takahiro Yasui ◽  
Kazumi Taguchi ◽  
Yasuhiko Hirose ◽  
Kazuhiro Niimi ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Kidney Stone ◽  
Stone Formation ◽  
Calcium Oxalate Crystals ◽  
In Vivo Models ◽  
Oxalate Crystals ◽  
Kidney Stone Formation

The controversial association of gut and urinary microbiota with kidney stone formation

2021 ◽  
pp. 105257
Author(s):  
Narjess Bostanghadiri ◽  
Pardis Ziaeefar ◽  
Fatemeh Sameni ◽  
Mohammad Mahmoudi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Kidney Stone ◽  
Stone Formation ◽  
Urinary Microbiota ◽  
Kidney Stone Formation
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