scholarly journals Bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
René H. M. Raeven ◽  
Jolanda Brummelman ◽  
Jeroen L. A. Pennings ◽  
Larissa van der Maas ◽  
Wichard Tilstra ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Inflammatory Responses ◽  
Membrane Vesicle ◽  
Cell Vaccine ◽  
Outer Membrane Vesicle ◽  
Whole Cell ◽  
Whole Cell Vaccine ◽  
Equal Efficacy

scholarly journals The Role of Virulence Proteins in Protection Conferred by Bordetella pertussis Outer Membrane Vesicle Vaccines

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 429
Author(s):  
René H. M. Raeven ◽  
Naomi van Vlies ◽  
Merijn L. M. Salverda ◽  
Larissa van der Maas ◽  
Joost P. Uittenbogaard ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Bordetella Pertussis ◽  
Protective Immunity ◽  
Membrane Vesicle ◽  
Outer Membrane Proteins ◽  
Outer Membrane Vesicle ◽  
Protective Capacity ◽  
T Helper 1 ◽  
Virulence Proteins ◽  
Humoral Responses

The limited protective immunity induced by acellular pertussis vaccines demands development of novel vaccines that induce broader and longer-lived immunity. In this study, we investigated the protective capacity of outer membrane vesicle pertussis vaccines (omvPV) with different antigenic composition in mice to gain insight into which antigens contribute to protection. We showed that total depletion of virulence factors (bvg(-) mode) in omvPV led to diminished protection despite the presence of high antibody levels. Antibody profiling revealed overlap in humoral responses induced by vaccines in bvg(-) and bvg(+) mode, but the potentially protective responses in the bvg(+) vaccine were mainly directed against virulence-associated outer membrane proteins (virOMPs) such as BrkA and Vag8. However, deletion of either BrkA or Vag8 in our outer membrane vesicle vaccines did not affect the level of protection. In addition, the vaccine-induced immunity profile, which encompasses broad antibody and mixed T-helper 1, 2 and 17 responses, was not changed. We conclude that the presence of multiple virOMPs in omvPV is crucial for protection against Bordetella pertussis. This protective immunity does not depend on individual proteins, as their absence or low abundance can be compensated for by other virOMPs.

scholarly journals Fha Deficient Bordetella pertussis Isolates in Iran with 50 Years Whole Cell Pertussis Vaccination

2021 ◽  
Author(s):  
Samaneh Saedi ◽  
Azadeh Safarchi ◽  
Faranak Tayebzadeh Moghadam ◽  
Siamak Heidarzadeh ◽  
Vajihe Sadat Nikbin ◽  
...  
Keyword(s):  
Western Blot ◽  
Virulence Factors ◽  
Bordetella Pertussis ◽  
Cell Vaccine ◽  
Reference Laboratory ◽  
Immunization Program ◽  
Whole Cell ◽  
Filamentous Hemagglutinin ◽  
Whole Cell Vaccine ◽  
Acellular Vaccine

Background: Bordetella pertussis, a highly contagious respiratory. Notably, the resurgence of pertussis has recently been associated with the lacking production of vaccine virulence factors. This study aimed to screen pertactin (Prn) and filamentous hemagglutinin (Fha) production in Iran with 50 years' whole cell vaccine (WCV) immunization program. Methods: Overall, 130 B. pertussis isolates collected from Pertussis Reference Laboratory of Iran during 2005-2018. Real-time PCR was performed by targeting IS481, ptxP, IS1001 and IS1002 for species confirmation of B. pertussis. Western-blot was used to evaluate the expression of virulence factors (pertactin and filamentous hemagglutinin). Results: All tested B. pertussis isolates expressed Prn and all except two isolates expressed Fha. We have sequenced genomes of these strains and identified differences compared with genome reference B. pertussis Tohama I. Conclusion: Many countries reporting Prn and Fha-deficiency due to acellular vaccine (ACV) pressure. Our results demonstrate in a country with WCV history, Fha-deficient isolates may rise independently. However, Prn-deficient isolates are more under the ACV pressure in B. pertussis isolates. Continues surveillance will provide a better understanding of the effect of WCV on the evolution of the pathogen deficiency.  

scholarly journals Antibody Responses to Bordetella pertussis Fim2 or Fim3 following Immunization with a Whole-Cell, Two-Component, or Five-Component Acellular Pertussis Vaccine and following Pertussis Disease in Children in Sweden in 1997 and 2007

2013 ◽  
Vol 21 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Hans Hallander ◽  
Abdolreza Advani ◽  
Frances Alexander ◽  
Lennart Gustafsson ◽  
Margaretha Ljungman ◽  
...  
Keyword(s):  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Geometric Mean ◽  
Acellular Pertussis Vaccine ◽  
Antibody Responses ◽  
Cell Vaccine ◽  
Whole Cell ◽  
Content Type ◽  
Two Component ◽  
Whole Cell Vaccine

ABSTRACTBordetella pertussisfimbriae (Fim2 and Fim3) are components of a five-component acellular pertussis vaccine (diphtheria–tetanus–acellular pertussis vaccine [DTaP5]), and antibody responses to fimbriae have been associated with protection. We analyzed the IgG responses to individual Fim2 and Fim3 in sera remaining from a Swedish placebo-controlled efficacy trial that compared a whole-cell vaccine (diphtheria-tetanus-whole-cell pertussis vaccine [DTwP]), a two-component acellular pertussis vaccine (DTaP2), and DTaP5. One month following three doses of the Fim-containing vaccines (DTwP or DTaP5), anti-Fim2 geometric mean IgG concentrations were higher than those for anti-Fim3, with a greater anti-Fim2/anti-Fim3 IgG ratio elicited by DTaP5. We also determined the responses in vaccinated children following an episode of pertussis. Those who received DTaP5 showed a large rise in anti-Fim2 IgG, reflecting the predominant Fim2 serotype at the time. In contrast, those who received DTwP showed an equal rise in anti-Fim2 and anti-Fim3 IgG concentrations, indicating that DTwP may provide a more efficient priming effect for a Fim3 response following contact withB. pertussis. Anti-Fim2 and anti-Fim3 IgG concentrations were also determined in samples from two seroprevalence studies conducted in Sweden in 1997, when no pertussis vaccine was used and Fim2 isolates predominated, and in 2007, when either DTaP2 or DTaP3 without fimbriae was used and Fim3 isolates predominated. Very similar distributions of anti-Fim2 and anti-Fim3 IgG concentrations were obtained in 1997 and 2007, except that anti-Fim3 concentrations in 1997 were lower. This observation, together with the numbers of individuals with both anti-Fim2 and anti-Fim3 IgG concentrations, strongly suggests thatB. pertussisexpresses both Fim2 and Fim3 during infection.

scholarly journals Inhibition of Inflammation and Mucus Production By Bordetella Pertussis Whole-Cell Vaccine in a Murine Model of Allergic Rhinitis

2016 ◽  
Vol 137 (2) ◽  
pp. AB27
Author(s):  
Marcelo Vivolo Aun ◽  
Fernanda Arantes-Costa ◽  
Francine Maria Almeida ◽  
Thayse Regina Brüggermann ◽  
Beatriz Mangueira Saraiva-Romanholo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Bordetella Pertussis ◽  
Murine Model ◽  
Cell Vaccine ◽  
Mucus Production ◽  
Whole Cell ◽  
Whole Cell Vaccine

scholarly journals Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine

2019 ◽  
Vol 8 (1) ◽  
pp. 1416-1427 ◽  
Author(s):  
Azadeh Safarchi ◽  
Sophie Octavia ◽  
Vajihe Sadat Nikbin ◽  
Masoumeh Nakhost Lotfi ◽  
Seyed Mohsen Zahraei ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Cell Vaccine ◽  
Whole Cell ◽  
Genomic Epidemiology ◽  
Whole Cell Vaccine

scholarly journals Prevalence and characterization of pertactin deficient Bordetella pertussis strains in Brazil, a whole-cell vaccine country

Vaccine X ◽  
2021 ◽  
pp. 100103
Author(s):  
Daniela Leite ◽  
Carlos Henrique Camargo ◽  
Suely Sanae Kashino ◽  
Ricardo Polatto ◽  
Luciano Moura Martins ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Cell Vaccine ◽  
Whole Cell ◽  
Whole Cell Vaccine
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